• 1 第四軍醫(yī)大學(xué)唐都醫(yī)院呼吸科（陜西西安 710038）;;
  • 2 解放軍253醫(yī)院急診科（內(nèi)蒙古呼和浩特 010051）;;
  • 3 第四軍醫(yī)大學(xué)西京醫(yī)院呼吸科（陜西西安 710032）;

目的  探討血管緊張素轉(zhuǎn)換酶( ACE) 和ACE2 在煙霧吸入傷后大鼠肺、腎的表達(dá)變化和可能的作用機(jī)制。方法  30 只健康雄性SD 大鼠隨機(jī)分為正常對(duì)照組, 傷后1、4、10 和24 h 組, 每組各6 只。致傷組予以濃煙吸入30 min, 按組別不同在相應(yīng)時(shí)間點(diǎn)取其肺、腎組織, 分別進(jìn)行肺組織濕/ 干重測定、HE染色和ACE、ACE2 免疫組織化學(xué)檢測。結(jié)果  致傷組大鼠均表現(xiàn)出急性肺損傷癥狀, 肺濕/ 干重均顯著高于正常對(duì)照組( P  lt;0. 05 或P  lt;0. 01) 。ACE、ACE2 在正常肺、腎細(xì)胞膜上有表達(dá)。肺內(nèi)ACE 表達(dá)在致傷后立即升高; 而肺內(nèi)ACE2 在致傷后4 h 開始明顯升高。各組間腎組織ACE 及ACE2 表達(dá)無明顯差別。結(jié)論  肺組織ACE、ACE2 表達(dá)的不同變化在煙霧吸入傷的發(fā)生機(jī)制中可能有重要作用。

引用本文： 高維,金發(fā)光,李圓園,保鵬濤. 煙霧吸入傷后大鼠肺及腎血管緊張素轉(zhuǎn)換酶表達(dá)變化的研究. 中國呼吸與危重監(jiān)護(hù)雜志, 2009, 09(1): 63-67. doi: 復(fù)制

1. Tipnis SR, Hooper NM, Hyde R, et al. A human homolog of angiotensin-converting enzyme. Cloning and functional expression as a captopril-insensitive carboxypeptidase. J Biol Chem, 2000, 275 :33238-33243.
2. Donoghue M, Hsieh F, Baronas E, et al. A novel angiotensin-converting enzyme-related carboxypeptidase ( ACE2 ) converts angiotensin I to angiotensin 1-9. Circ Res, 2000, 87: 523-525.
3. Kuba K, Imai Y, Penninger JM. Angiotensin-converting enzyme 2 in lung diseases. Curr Opin Pharmacol, 2006, 6: 271-276.
4. 謝爾凡, 楊宗城, 王孰, 等. 大鼠煙霧吸入性損傷模型的制作.中國實(shí)驗(yàn)動(dòng)物學(xué)雜志, 1994, 4: 219-222.
5. Imai Y, Kuba K, Penninger JM. The discovery of angiotensin-converting enzyme 2 and its role in acute lung injury in mice, Exp Physiol,2008, 93: 543-548.
6. 張維, 王導(dǎo)新. 肺泡上皮細(xì)胞與急性肺損傷水腫液清除的研究進(jìn)展. 中國呼吸與危重監(jiān)護(hù)雜志, 2008, 1: 74-77.
7. Marshall RP, Webb S, Bellingan GJ, et al. Angiotensin converting enzyme insertion/deletion polymorphism is associated with susceptibility and outcome in acute respiratory distress syndrome. Am J Respir Crit Care Med, 2002, 166: 646-650.
8. Brizio-Molteni L, Piano G, Warpeha RL, et al. Angiotensin-1 -converting enzyme activity as index of pulmonary damage in thermal injury with or without smoke inhalation. Ann Clin Lab Sci, 1992, 22 : 1-10.
9. Imai Y, Kuba K, Rao S, et al. Angiotensin-converting enzyme 2 protects from severe acute lung failure. Nature, 2005, 436: 112-116.
10. Skeggs LT, Dorer FE, Lecine M, et al. The biochemistry of the reninangiotensin system. Adv Exp Med Biol, 1980, 130: 1-27.
11. Corvol P, Williams TA, Soubrier F. Peptidyl dipeptidase A: angiotensin I-converting enzyme. Methods Enzymol, 1995, 248: 283-305.
12. Crackower MA, Sarao R, Oudit GY, et al. Angiotensin-converting enzyme 2 is an essential regulator of heart function. Nature, 2002 ,417: 822-828.
13. Yamamoto K, Ohishi M, Katsuya T, et al. Deletion of angiotensinconverting enzyme 2 accelerates pressure overload-induced cardiac dysfunction by increasing local angiotensin II. Hypertension, 2006 ,47: 718-726.
14. Imai Y, Kuba K, Penninger JM. The renin-angiotensin system in acute respiratory distress syndrome. Drug Discovery Today: Disease Mechanisms, 2006, 2: 225-229.
15. Rivière G, Michaud A, Breton C, et al. Angiotensin-converting enzyme 2 ( ACE2) and ACE activities display tissue-specific sensitivity to undernutrition-programmed hypertension in the adult rat. Hypertension,2005, 46: 1169-1174.
16. 史家欣, 趙中蘇. 腎素-血管緊張素系統(tǒng)和應(yīng)激在延誤吸入性急性肺損傷中的作用. 西北國防醫(yī)學(xué)雜志, 2006, 27: 214-216.
17. Koka V, Huang XR, Chung AC, et al. Angiotensin II up-regulates angiotensin I-converting enzyme ( ACE) , but down-regulates ACE2 via the AT1-ERK/p38 MAP kinase pathway. Am J Pathol, 2008,172: 1174-1183.
  1. 1. Tipnis SR, Hooper NM, Hyde R, et al. A human homolog of angiotensin-converting enzyme. Cloning and functional expression as a captopril-insensitive carboxypeptidase. J Biol Chem, 2000, 275 :33238-33243.
  2. 2. Donoghue M, Hsieh F, Baronas E, et al. A novel angiotensin-converting enzyme-related carboxypeptidase ( ACE2 ) converts angiotensin I to angiotensin 1-9. Circ Res, 2000, 87: 523-525.
  3. 3. Kuba K, Imai Y, Penninger JM. Angiotensin-converting enzyme 2 in lung diseases. Curr Opin Pharmacol, 2006, 6: 271-276.
  4. 4. 謝爾凡, 楊宗城, 王孰, 等. 大鼠煙霧吸入性損傷模型的制作.中國實(shí)驗(yàn)動(dòng)物學(xué)雜志, 1994, 4: 219-222.
  5. 5. Imai Y, Kuba K, Penninger JM. The discovery of angiotensin-converting enzyme 2 and its role in acute lung injury in mice, Exp Physiol,2008, 93: 543-548.
  6. 6. 張維, 王導(dǎo)新. 肺泡上皮細(xì)胞與急性肺損傷水腫液清除的研究進(jìn)展. 中國呼吸與危重監(jiān)護(hù)雜志, 2008, 1: 74-77.
  7. 7. Marshall RP, Webb S, Bellingan GJ, et al. Angiotensin converting enzyme insertion/deletion polymorphism is associated with susceptibility and outcome in acute respiratory distress syndrome. Am J Respir Crit Care Med, 2002, 166: 646-650.
  8. 8. Brizio-Molteni L, Piano G, Warpeha RL, et al. Angiotensin-1 -converting enzyme activity as index of pulmonary damage in thermal injury with or without smoke inhalation. Ann Clin Lab Sci, 1992, 22 : 1-10.
  9. 9. Imai Y, Kuba K, Rao S, et al. Angiotensin-converting enzyme 2 protects from severe acute lung failure. Nature, 2005, 436: 112-116.
  10. 10. Skeggs LT, Dorer FE, Lecine M, et al. The biochemistry of the reninangiotensin system. Adv Exp Med Biol, 1980, 130: 1-27.
  11. 11. Corvol P, Williams TA, Soubrier F. Peptidyl dipeptidase A: angiotensin I-converting enzyme. Methods Enzymol, 1995, 248: 283-305.
  12. 12. Crackower MA, Sarao R, Oudit GY, et al. Angiotensin-converting enzyme 2 is an essential regulator of heart function. Nature, 2002 ,417: 822-828.
  13. 13. Yamamoto K, Ohishi M, Katsuya T, et al. Deletion of angiotensinconverting enzyme 2 accelerates pressure overload-induced cardiac dysfunction by increasing local angiotensin II. Hypertension, 2006 ,47: 718-726.
  14. 14. Imai Y, Kuba K, Penninger JM. The renin-angiotensin system in acute respiratory distress syndrome. Drug Discovery Today: Disease Mechanisms, 2006, 2: 225-229.
  15. 15. Rivière G, Michaud A, Breton C, et al. Angiotensin-converting enzyme 2 ( ACE2) and ACE activities display tissue-specific sensitivity to undernutrition-programmed hypertension in the adult rat. Hypertension,2005, 46: 1169-1174.
  16. 16. 史家欣, 趙中蘇. 腎素-血管緊張素系統(tǒng)和應(yīng)激在延誤吸入性急性肺損傷中的作用. 西北國防醫(yī)學(xué)雜志, 2006, 27: 214-216.
  17. 17. Koka V, Huang XR, Chung AC, et al. Angiotensin II up-regulates angiotensin I-converting enzyme ( ACE) , but down-regulates ACE2 via the AT1-ERK/p38 MAP kinase pathway. Am J Pathol, 2008,172: 1174-1183.

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