目的 探討建立不同間歇低氧( IH) 程度、IH 時(shí)間和再氧合( ROX) 時(shí)間以及持續(xù)低氧( CH) 模式家兔在體頸動(dòng)脈體和頸總動(dòng)脈模型。方法 由計(jì)算機(jī)程序控制單片機(jī), 在串口通訊協(xié)議驅(qū)動(dòng)下, 由下位機(jī)芯片控制不同氧分壓( PO2 ) 灌注液的灌注過(guò)程。45 只成年雄性新西蘭大耳白家兔( 2. 5 ～3. 0 kg) 復(fù)合麻醉后保留自主呼吸, 游離右側(cè)頸總動(dòng)脈和竇神經(jīng)( CSN) , 顯露CSN化學(xué)感受性神經(jīng)細(xì)束并安放電極記錄CSN傳入活性。結(jié)扎右側(cè)頸總動(dòng)脈向心端血流, 并向右側(cè)頸總動(dòng)脈遠(yuǎn)心端內(nèi)插入導(dǎo)管, 經(jīng)蠕動(dòng)輸液泵交替灌注以發(fā)泡法預(yù)平衡的灌注液( 2 mL/min) 。灌注低氧灌注液, 而后再灌注正常氧灌注液, 交替往復(fù)形成IH/ROX 循環(huán), 從而在頸總動(dòng)脈內(nèi)模擬出睡眠呼吸暫停狀態(tài)的不同IH 模式IH/ROX 暴露情況; 或持續(xù)灌注低氧液以模擬CH 暴露情況。監(jiān)測(cè)頸動(dòng)脈體CSN傳入活性, 完成暴露后游離收集頸動(dòng)脈體和插管遠(yuǎn)心端的頸總動(dòng)脈及其分叉作為標(biāo)本。結(jié)果 正常氧灌注時(shí)CSN化學(xué)傳入細(xì)束的基線頻率為( 0.17 ±0. 03) 次脈沖/ s( impulse / s) , 平均波幅為( 46. 2 ±4. 4) μV; 低氧灌注時(shí)傳入頻率為( 0. 64 ±0. 09) impulse/ s, 平均波幅為( 87. 4 ±6. 6) μV; 正常氧灌注液的PO2 約為( 139 ±1. 5) mmHg, 而IH 灌注液的PO2 約為( 35. 2 ±1. 3) mmHg。結(jié)論 該模型可以用于研究不同IH 和CH 模式時(shí)頸動(dòng)脈體和頸總動(dòng)脈多種神經(jīng)和生物化學(xué)變化, 具有較大的應(yīng)用價(jià)值。
引用本文： 馮靖,崔林陽(yáng),陳寶元,郭美南,曹潔,孫蓓. 不同間歇低氧與持續(xù)低氧模式家兔在體頸動(dòng)脈體和頸總動(dòng)脈模型的建立. 中國(guó)呼吸與危重監(jiān)護(hù)雜志, 2009, 09(4): 380-383. doi: 復(fù)制
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| 1. | Shamsuzzaman AS, Gersh BJ, Somers VK. Obstructive sleep apnea:implications for cardiac and vascular disease. JAMA, 2003, 290 :1906-1914. |
| 2. | Fletcher EC. Sympathetic over activity in the etiology of hypertension of obstructive sleep apnea. Sleep, 2003, 26: 15-19 . |
| 3. | Narkiewicz K, van de Borne PJ, Pesek CA, et a1. Selective potentiation of peripheral chemoreflex sensitivity in obstructive sleep apnea. Circulation, 1999, 99: 1183 -1189 . |
| 4. | Prabhakar NR, Peng YJ, Jacono FJ, et a1. Cardiovascular alterations bv chronic intermittent hypoxia: importance of carotid body chemoreflexes. Clin Exp Pharmacol Physiol, 2005 , 32, 447 -449. |
| 5. | Williams A, Scharf SM. Obstructive sleep apnea, cardiovascular disease, and inflammation-is NF-κB the key? Sleep Breath, 2007 ,11: 69-76. |
| 6. | 馮靖, 陳寶元, 郭美南, 等. 內(nèi)皮細(xì)胞在不同低氧模式下白細(xì)胞介素6 和腫瘤壞死因子α的變化. 中華醫(yī)學(xué)雜志, 2007, 87:774-777. |
| 7. | 馮靖, 陳寶元, 郭美南, 等. 內(nèi)皮細(xì)胞在不同間歇缺氧方式時(shí)核因子κB 和細(xì)胞間粘附分子1 的變化. 中華結(jié)核和呼吸雜志,2007, 30: 202-206. |
| 8. | 馮靖, 陳寶元, 郭美南, 等. 不同間歇低氧頻率對(duì)血管內(nèi)皮細(xì)胞的炎性損傷. 中華老年醫(yī)學(xué)雜志, 2007, 26: 124-126. |
| 9. | Cummings KJ, Wilson RJ. Time-dependent modulation of carotid body afferent activity during and after intermittent hypoxia. Am J Physiol Regul Integr Comp Physiol, 2005, 288: R1571-R1580. |
| 10. | Peng YJ, Yuan G, Ramakrishnan D, et a1. Heterozygous HIF-1 alpha deficiency impairs carotid body-mediated systemic responses and reactive oxygen species generation in mice exposed to intermittent hypoxia. J Physiol, 2006, 577: 705-716. |
- 1. Shamsuzzaman AS, Gersh BJ, Somers VK. Obstructive sleep apnea:implications for cardiac and vascular disease. JAMA, 2003, 290 :1906-1914.
- 2. Fletcher EC. Sympathetic over activity in the etiology of hypertension of obstructive sleep apnea. Sleep, 2003, 26: 15-19 .
- 3. Narkiewicz K, van de Borne PJ, Pesek CA, et a1. Selective potentiation of peripheral chemoreflex sensitivity in obstructive sleep apnea. Circulation, 1999, 99: 1183 -1189 .
- 4. Prabhakar NR, Peng YJ, Jacono FJ, et a1. Cardiovascular alterations bv chronic intermittent hypoxia: importance of carotid body chemoreflexes. Clin Exp Pharmacol Physiol, 2005 , 32, 447 -449.
- 5. Williams A, Scharf SM. Obstructive sleep apnea, cardiovascular disease, and inflammation-is NF-κB the key? Sleep Breath, 2007 ,11: 69-76.
- 6. 馮靖, 陳寶元, 郭美南, 等. 內(nèi)皮細(xì)胞在不同低氧模式下白細(xì)胞介素6 和腫瘤壞死因子α的變化. 中華醫(yī)學(xué)雜志, 2007, 87:774-777.
- 7. 馮靖, 陳寶元, 郭美南, 等. 內(nèi)皮細(xì)胞在不同間歇缺氧方式時(shí)核因子κB 和細(xì)胞間粘附分子1 的變化. 中華結(jié)核和呼吸雜志,2007, 30: 202-206.
- 8. 馮靖, 陳寶元, 郭美南, 等. 不同間歇低氧頻率對(duì)血管內(nèi)皮細(xì)胞的炎性損傷. 中華老年醫(yī)學(xué)雜志, 2007, 26: 124-126.
- 9. Cummings KJ, Wilson RJ. Time-dependent modulation of carotid body afferent activity during and after intermittent hypoxia. Am J Physiol Regul Integr Comp Physiol, 2005, 288: R1571-R1580.
- 10. Peng YJ, Yuan G, Ramakrishnan D, et a1. Heterozygous HIF-1 alpha deficiency impairs carotid body-mediated systemic responses and reactive oxygen species generation in mice exposed to intermittent hypoxia. J Physiol, 2006, 577: 705-716.