目的 探討半夏提取物( EP) 對(duì)脂多糖誘導(dǎo)的大鼠氣道黏液高分泌模型的干預(yù)作用。方法 30 只Wistar 大鼠隨機(jī)分為5 組( n= 6) , 分別為空白組、模型組、低劑量EP 組、中劑量EP 組及高劑量EP 組。模型組和EP 組經(jīng)氣道注入脂多糖( LPS) 復(fù)制大鼠氣道黏液高分泌模型。低、中、高劑量EP 組連續(xù)4 d 分別給予管飼EP 10、30 及60 g/kg; 采用免疫組化法檢測(cè)肺內(nèi)氣道的黏蛋白5AC ( MUC5AC) 蛋白表達(dá), RT-PCR 法檢測(cè)肺組織MUC5AC mRNA、水通道蛋白5( AQP-5) mRNA; ELISA 法檢測(cè)BALF 中的TNF-α濃度。結(jié)果 大鼠氣道上皮MUC5AC 蛋白和肺組織MUC5AC mRNA 的表達(dá)在模型組中均明顯高于空白組( P lt; 0. 05) ; 在低劑量及中劑量EP 組較模型組稍降低( P gt;0. 05) , 仍顯著高于空白組( P lt; 0. 05) ; 在高劑量EP 組顯著低于模型組( P lt;0. 05) 。大鼠AQP-5 mRNA 表達(dá)在模型組顯著低于空白組( P lt;0. 05) ; 在低劑量及中劑量EP 組較模型組稍升高( P gt; 0. 05) , 仍較空白組顯著降低( P lt;0. 05) ; 在高劑量EP 組顯著高于模型組( P lt; 0. 01) 。BALF 中TNF-α濃度與肺組織MUC5AC mRNA 的表達(dá)呈正相關(guān)( r = 0. 948, P lt;0. 05) , 肺組織AQP-5 mRNA 與MUC5AC mRNA 及 TNF-α的表達(dá)呈負(fù)相關(guān)( r = - 0. 955, P lt;0. 05; r = - 0. 909, P lt;0. 05) 。結(jié)論 高劑量的EP 能明顯抑制大鼠氣道上黏液高分泌狀態(tài)。
引用本文： 鄧青南,周建龍,郭振輝,張萍. 半夏提取物對(duì)氣道黏液高分泌的影響. 中國(guó)呼吸與危重監(jiān)護(hù)雜志, 2009, 09(5): 477-481. doi: 復(fù)制
| 1. | 王可, 馮玉麟, 文富強(qiáng), 等. 哮喘患者氣道上皮鈣激活的氯離子通道1 表達(dá)增加與黏液高分泌. 中國(guó)呼吸與危重監(jiān)護(hù)雜志, 2005, 4: 96-99. |
| 2. | 王可, 馮玉麟, 文富強(qiáng), 等. 慢性阻塞性肺疾病患者氣道上皮水通道5 的表達(dá)與黏液高分泌. 中國(guó)呼吸與危重監(jiān)護(hù)雜志, 2006, ·480· 中國(guó)呼吸與危重監(jiān)護(hù)雜志2009 年9 月第8 卷第5 期Chin J Respir Crit Care Med, September 2009 , Vol. |
| 3. | Leikauf GD, Borchers MT, Prows DR, et al . Mucin apoprotein expression in COPD. Chest, 2002, 121: 166S-182S. |
| 4. | 鐘正賢, 陳學(xué)芬, 周桂芬, 等. 水半夏提取物的抗炎抗過敏作用研究. 中藥藥理與臨床, 2003, 19: 25-27. |
| 5. | Chen JH, Cui GY, Liu JY, et al. Pinelloside, an antimicrobial cerebroside from Pinellia ternata. Internationalimmunopharmacology, 2001 , 10 : 1353-1365. |
| 6. | ? 2002-2009 Editorial Department of Chinese Journal of Respiratory and Critical Care Medicine. All rights reserved. Http://www.cjrccm.com 5: 357-361. |
| 7. | 楊秀偉, 韓美華, 鐘國(guó)躍, 等. 半夏中抑制腫瘤壞死因子-α產(chǎn)生的新腦苷. 中草藥, 2008, 39: 485-489. |
| 8. | Naga T, Arai Y, Emori M, et al. Anti-allergic activity of a Kampo medicine “Sho-seiryu-to ” on airway inflammation in a mouse model. International-immunopharmacology, 2001, 10: 1353-1365. |
| 9. | 張科衛(wèi), 吳皓. 半夏中總游離有機(jī)酸的作用研究. 南京中醫(yī)藥大學(xué)學(xué)報(bào), 2001, 17: 159-1611. |
| 10. | , No. |
| 11. | Yanagihara K, Seki M, Cheng PW. Lipopolysaccharide induces mucus cell metaplasia in mouse lung . AmJ Reapir Cell Moll Biol, 2001, 24: 66-73. |
| 12. | Nagai T, Kiyohara H, Munakata K, et al. Pinellic acid from the tuber of Pinellia ternata Breitenbach as an effective oral adjuvant for nasal influenza vaccine. International-immunopharmacology, 2002 , 4: 1183-1193. |
| 13. | Kim JH, Lee SY, Bak SM, et al. Effects of matrix metalloproteinase inhibitor in LPS-induced goblet cell metaplasia. Am JPhysiol Lung Cell Mol Physiol, 2004, 28: L127-L133. |
| 14. | Song YL, Verkman AS. Aquaporin-5 dependent fluid secretion in airway submucosal glangs. J Biolo Chemis, 2001, 276: 41288- 41292. |
| 15. | Towne JE, Krane CM, Bachurski CJ, et al. Tumor necrosis Factoralpha inhibits aquaporin 5 expression in mouse lung Epithelial cells. J Biol Chem, 2001, 276: 18657-18664. |
| 16. | Che Z, Zhu R, Bai L, et al. Downregulation of aquaporin 5 Induced by vector-based short hairpin RNA and its effect on MUC5AC gene expression in human airway submucosal gland cells. Respir Physiol Neurobiol, 2006, 152: 197-203. |
- 1. 王可, 馮玉麟, 文富強(qiáng), 等. 哮喘患者氣道上皮鈣激活的氯離子通道1 表達(dá)增加與黏液高分泌. 中國(guó)呼吸與危重監(jiān)護(hù)雜志, 2005, 4: 96-99.
- 2. 王可, 馮玉麟, 文富強(qiáng), 等. 慢性阻塞性肺疾病患者氣道上皮水通道5 的表達(dá)與黏液高分泌. 中國(guó)呼吸與危重監(jiān)護(hù)雜志, 2006, ·480· 中國(guó)呼吸與危重監(jiān)護(hù)雜志2009 年9 月第8 卷第5 期Chin J Respir Crit Care Med, September 2009 , Vol.
- 3. Leikauf GD, Borchers MT, Prows DR, et al . Mucin apoprotein expression in COPD. Chest, 2002, 121: 166S-182S.
- 4. 鐘正賢, 陳學(xué)芬, 周桂芬, 等. 水半夏提取物的抗炎抗過敏作用研究. 中藥藥理與臨床, 2003, 19: 25-27.
- 5. Chen JH, Cui GY, Liu JY, et al. Pinelloside, an antimicrobial cerebroside from Pinellia ternata. Internationalimmunopharmacology, 2001 , 10 : 1353-1365.
- 6. ? 2002-2009 Editorial Department of Chinese Journal of Respiratory and Critical Care Medicine. All rights reserved. Http://www.cjrccm.com 5: 357-361.
- 7. 楊秀偉, 韓美華, 鐘國(guó)躍, 等. 半夏中抑制腫瘤壞死因子-α產(chǎn)生的新腦苷. 中草藥, 2008, 39: 485-489.
- 8. Naga T, Arai Y, Emori M, et al. Anti-allergic activity of a Kampo medicine “Sho-seiryu-to ” on airway inflammation in a mouse model. International-immunopharmacology, 2001, 10: 1353-1365.
- 9. 張科衛(wèi), 吳皓. 半夏中總游離有機(jī)酸的作用研究. 南京中醫(yī)藥大學(xué)學(xué)報(bào), 2001, 17: 159-1611.
- 10. , No.
- 11. Yanagihara K, Seki M, Cheng PW. Lipopolysaccharide induces mucus cell metaplasia in mouse lung . AmJ Reapir Cell Moll Biol, 2001, 24: 66-73.
- 12. Nagai T, Kiyohara H, Munakata K, et al. Pinellic acid from the tuber of Pinellia ternata Breitenbach as an effective oral adjuvant for nasal influenza vaccine. International-immunopharmacology, 2002 , 4: 1183-1193.
- 13. Kim JH, Lee SY, Bak SM, et al. Effects of matrix metalloproteinase inhibitor in LPS-induced goblet cell metaplasia. Am JPhysiol Lung Cell Mol Physiol, 2004, 28: L127-L133.
- 14. Song YL, Verkman AS. Aquaporin-5 dependent fluid secretion in airway submucosal glangs. J Biolo Chemis, 2001, 276: 41288- 41292.
- 15. Towne JE, Krane CM, Bachurski CJ, et al. Tumor necrosis Factoralpha inhibits aquaporin 5 expression in mouse lung Epithelial cells. J Biol Chem, 2001, 276: 18657-18664.
- 16. Che Z, Zhu R, Bai L, et al. Downregulation of aquaporin 5 Induced by vector-based short hairpin RNA and its effect on MUC5AC gene expression in human airway submucosal gland cells. Respir Physiol Neurobiol, 2006, 152: 197-203.