• 鄭州大學(xué)第二附屬醫(yī)院呼吸內(nèi)科（河南鄭州 450014）;

目的 觀察哮喘小鼠肺組織CCL1 /CCR8 mRNA 的表達(dá)及糖皮質(zhì)激素對(duì)其表達(dá)的影響, 探討CCL1 /CCR8 在哮喘發(fā)病機(jī)制中的作用。方法 30 只小鼠隨機(jī)分為對(duì)照組、哮喘組和地塞米松組, 每組10 只。哮喘組和地塞米松組用卵白蛋白致敏激發(fā)建立哮喘模型, 地塞米松組給予腹腔注射地塞米松( 2 mg/kg) , 對(duì)照組均以生理鹽水替代。測(cè)定BALF 中細(xì)胞總數(shù)和分類計(jì)數(shù), 采用酶聯(lián)免疫法檢測(cè)BALF 中IL-4 水平, RT-PCR 檢測(cè)肺組織CCR8 mRNA 和CCL1 mRNA 的表達(dá)。結(jié)果 對(duì)照組、哮喘組和地塞米松組BALF 中嗜酸粒細(xì)胞百分比[ ( 0. 6 ±0. 1) % 、( 32. 4 ±3. 4) % 和( 8.9 ±0. 9) % , P  lt;0. 01] 、淋巴細(xì)胞百分比[ ( 2.9 ±0. 5) % 、( 14. 0 ±3. 0) % 和( 6. 3 ±0. 6) % , P  lt;0. 01] 、IL-4濃度[ ( 5.16 ±1. 23) pg /mL、( 47. 67 ±11. 67) pg/mL 和( 19. 76 ±4. 72) pg/mL, P  lt;0. 01] 差異均有統(tǒng)計(jì)學(xué)意義, 其中哮喘組較對(duì)照組顯著增高( P  lt;0. 01) , 地塞米松組較哮喘組顯著降低但仍顯著高于對(duì)照組( P  lt;0. 01) 。對(duì)照組、哮喘組和地塞米松組CCL1 mRNA 表達(dá)( 0.11 ±0. 06、0. 76 ±0. 16 和0. 53 ±0. 13, P  lt;0. 01) 、CCR8 mRNA 表達(dá)( 0. 18 ±0. 09、1. 26 ±0. 13 和0. 79 ±0. 12, P  lt; 0. 01) 差異均有統(tǒng)計(jì)學(xué)意義, 其中哮喘組較對(duì)照組顯著增高( P  lt;0. 01) , 地塞米松組較哮喘組顯著降低但仍顯著高于對(duì)照組( P  lt;0. 01) 。結(jié)論CCL1/CCR8 在哮喘小鼠肺組織中的基因表達(dá)增強(qiáng)。糖皮質(zhì)激素可能通過減少CCL1 /CCR8 的基因表達(dá), 減輕哮喘氣道炎癥。

引用本文： 王澤宇,劉劍波. CCL1/CCR8在哮喘小鼠肺組織中的表達(dá)及地塞米松對(duì)其作用的研究. 中國呼吸與危重監(jiān)護(hù)雜志, 2010, 9(1): 32-35. doi: 復(fù)制

1. Heijink IH,Van Oosterhout AJ.Targeting T cells for asthma.Curr Opin Pharmacol,2005 ,5:227-231.
2. Zingoni A,Soto H,Hedrick JA,et al. The chemokine receptor CCR8 is preferentially expressed in Th2 but not Th1 cells.J Immunol,1998,161:547-551.
3. Gombert M, Dieu-Nosjean MC, Winterberg F,et al. CCL1-CCR8 interactions: an axis mediating the recruitment of T cells and Langerhans-type dendritic cells to sites of atopic skin inflammation. J Immunol, 2005,174:5082-5091.
4. Cantor J, Haskins K. Recruitment and activation of macrophages by pathogenic CD4 T cells in type 1 diabetes: evidence for involvement of CCR8 and CCL1.J Immunol,2007,79:5760-5767.
5. Larché M, Robinson DS, Kay AB. The role of T lymphocytes in the pathogenesis of asthma. J Allergy Clin Immunol,2003,111:450-463.
6. Laudanna C, Kim JY, Constantin G,et al. Rapid leukocyte integrin activation by chemokines. Immunol Rev,2002,186:37-46.
7. Moser B, Loetscher M,Piali L,et al.Lymphocyte responses to chemokines.Int Rev Immunol, 1998,16:323-344.
8. Gonzalo JA,Qiu Y,Lora JM,et al.Coordinated involvement of mast cells and T cells in allergic mucosal inflammation:critical role of the CC chemokine ligand 1:CCR8 axis.J Immunol, 2007,179:1740-1750.
9. Chensue SW,Lukacs NW,Yang TY,et al.Aberrant in vivo T helper type 2 cell response and impaired eosinophil recruitment in CC chemokine receptor 8 knockout mice. J Exp Med, 2001,193:573-584.
10. Chung CD, Kuo F, Kumer J,et al. CCR8 is not essential for the development of inflammation in a mouse model of allergic airway disease. J Immunol,2003,170:581-587.
11. Hoshino M,Nakagawa T,Sano Y,et al.Effect of inhaled corticosteroid on an immunoreactive thymus and activation-regulated chemokine expression in the bronchial biopsies from asthmatics. Allergy,2005,60:317-322.
12. 李鋒,劉榮玉.氣道炎癥和重構(gòu)在小鼠哮喘模型中的變化及地塞米松的干預(yù)作用。中國呼吸與危重監(jiān)護(hù)雜志.2005,4:110-114.
  1. 1. Heijink IH,Van Oosterhout AJ.Targeting T cells for asthma.Curr Opin Pharmacol,2005 ,5:227-231.
  2. 2. Zingoni A,Soto H,Hedrick JA,et al. The chemokine receptor CCR8 is preferentially expressed in Th2 but not Th1 cells.J Immunol,1998,161:547-551.
  3. 3. Gombert M, Dieu-Nosjean MC, Winterberg F,et al. CCL1-CCR8 interactions: an axis mediating the recruitment of T cells and Langerhans-type dendritic cells to sites of atopic skin inflammation. J Immunol, 2005,174:5082-5091.
  4. 4. Cantor J, Haskins K. Recruitment and activation of macrophages by pathogenic CD4 T cells in type 1 diabetes: evidence for involvement of CCR8 and CCL1.J Immunol,2007,79:5760-5767.
  5. 5. Larché M, Robinson DS, Kay AB. The role of T lymphocytes in the pathogenesis of asthma. J Allergy Clin Immunol,2003,111:450-463.
  6. 6. Laudanna C, Kim JY, Constantin G,et al. Rapid leukocyte integrin activation by chemokines. Immunol Rev,2002,186:37-46.
  7. 7. Moser B, Loetscher M,Piali L,et al.Lymphocyte responses to chemokines.Int Rev Immunol, 1998,16:323-344.
  8. 8. Gonzalo JA,Qiu Y,Lora JM,et al.Coordinated involvement of mast cells and T cells in allergic mucosal inflammation:critical role of the CC chemokine ligand 1:CCR8 axis.J Immunol, 2007,179:1740-1750.
  9. 9. Chensue SW,Lukacs NW,Yang TY,et al.Aberrant in vivo T helper type 2 cell response and impaired eosinophil recruitment in CC chemokine receptor 8 knockout mice. J Exp Med, 2001,193:573-584.
  10. 10. Chung CD, Kuo F, Kumer J,et al. CCR8 is not essential for the development of inflammation in a mouse model of allergic airway disease. J Immunol,2003,170:581-587.
  11. 11. Hoshino M,Nakagawa T,Sano Y,et al.Effect of inhaled corticosteroid on an immunoreactive thymus and activation-regulated chemokine expression in the bronchial biopsies from asthmatics. Allergy,2005,60:317-322.
  12. 12. 李鋒,劉榮玉.氣道炎癥和重構(gòu)在小鼠哮喘模型中的變化及地塞米松的干預(yù)作用。中國呼吸與危重監(jiān)護(hù)雜志.2005,4:110-114.