目的 研究聯(lián)合檢測微衛(wèi)星不穩(wěn)定性( MSI) 和p16 基因啟動子甲基化在早期肺癌中的診斷價(jià)值。方法 對肺癌、癌前病變和正常肺組織采用PCR 單鏈長度分析法檢測MSI, 采用甲基化特異PCR 法檢測p16 甲基化。結(jié)果 癌前病變組MSI 的發(fā)生率顯著高于肺癌組( P lt;0. 05) 和正常肺組織組( P lt;0. 01) , 肺癌組MSI 的發(fā)生率顯著高于正常肺組織組( P lt;0. 01) ; 肺癌組p16 甲基化的發(fā)生率顯著高于癌前病變組( P lt;0. 01) 和正常肺組織組( P lt;0. 01) , 癌前病變組p16 甲基化的發(fā)生率顯著高于正常肺組織組( P lt;0. 01) ; 聯(lián)合檢測MSI 和p16 甲基化的敏感性顯著高于單一檢測MSI( P lt;0. 01) 和p16 甲基化( P lt;0. 05) ; 聯(lián)合檢測法與單一檢測MSI 和單一檢測p16 甲基化的特異性比較,差異無顯著性意義( P gt;0. 05) 。結(jié)論 聯(lián)合檢測MSI 和p16 甲基化可以顯著提高早期肺癌診斷的敏感性同時(shí)不降低其特異性, 值得臨床推廣。
引用本文： 廖秀清,趙麟豐,余時(shí)滄,郭武,王朝永. 聯(lián)合檢測微衛(wèi)星不穩(wěn)定性和p16甲基化在早期肺癌中的診斷價(jià)值. 中國呼吸與危重監(jiān)護(hù)雜志, 2010, 9(1): 81-82. doi: 復(fù)制
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- 1. 張鋆歆,申洪.肺增生病變FHIT基因微衛(wèi)星不穩(wěn)定和雜合性丟失的研究.第一軍醫(yī)大學(xué)學(xué)報(bào),2003,23:313-317.
- 2. González-Quevedo R,García-Aranda C,Morán A,et al. Differential impact of p16 inactivation by promoter methylation in non-small cell lung and colorectal cancer:clinical implications. Int J Oncol,2004,24:349-355.
- 3. Kwong FM，Tang JC，Srivastava G，et a1. Inactivation mechanisms and growth suppressive effects of p16 INK4a in Asian esophageal squamous carcinoma cell lines[J]. Cancer Lett，2004，208: 207-213.
- 4. Dacic S,Lomago D,Hunt JL,et al. Microsatellite instability is uncommon in lymphoepithelioma-like carcinoma of the lung.Am J Clin Pathol,2007,127:282-286.
- 5. Melloni G,Doglioni C,Bandiera A,et al. Prognostic factors and analysis of microsatellite instability in resected pulmonary metastases from colorectal carcinoma. Ann Thorac Surg,Ann Thorac Surg,2006,81:2008-2013.
- 6. Hung J,Kishimoto Y,Sugio K,et al.Allele-specific chromosome 3p deletions occur at an early stage in the pathogenesis of lung carcinoma.JAMA,1995,273:558-563.
- 7. Malhotra P,Behera D,Srinivasan R,et a1.Detection of microsatellite alterations in bronchial washings in squamous cell lung cancer: the first study from India.Jpn J Clin Oncol,2004,34:439-444.
- 8. Bodner-Adler B,Bodner K,Czerwenka K,et al. Expression of p16 protein in patients with uterine smooth muscle tumors: an immunohistochemical analysis. Gynecol Oncol,2005,96:62-66.
- 9. Georgiou E,Valeri R,Tzimagiorgis G,et al.Aberrant p16 promoter methylation among Greek lung cancer patients and smokers: correlation with smoking. Eur J Cancer Prev,2007,16:396-402.
- 10. Palmisano WA,Divine KK,Saccomanno G,et al.Predicting lung cancer by detecting aberrant promoter methylation in sputum.Cancer Res,2000,60:5954-5958.