目的 研究COPD 大鼠膈肌組織內(nèi)蛋白降解途徑之一的泛素-蛋白酶體系統(tǒng)組成成分的變化及意義。方法 采用單純熏香煙法復(fù)制COPD 大鼠動(dòng)物模型。蛋白免疫印記( Western blot)方法測(cè)定大鼠膈肌內(nèi)E2-14k、蛋白酶體C8 亞基的蛋白表達(dá); 逆轉(zhuǎn)錄-聚合酶鏈?zhǔn)椒磻?yīng)( RT-PCR) 技術(shù)檢測(cè)膈肌內(nèi)泛素和蛋白酶體C2 亞基的mRNA 表達(dá)。結(jié)果 Western blot 分析結(jié)果顯示, COPD 大鼠膈肌組織中E2-14k 的蛋白表達(dá)較正常對(duì)照組顯著增強(qiáng)( 0. 81 ±0. 28 比0. 50 ±0. 25, P lt;0. 05) , 蛋白酶體C8 亞基的蛋白表達(dá)變化不明顯( P gt;0. 05) ; RT-PCR 分析結(jié)果顯示, 膈肌組織中泛素的mRNA 表達(dá)( IOD值) 較對(duì)照組顯著增加( 0. 89 ±0. 20 比0. 50 ±0. 15, P lt;0. 05) , 蛋白酶體C2 亞基的mRNA 表達(dá)
與正常對(duì)照組比較無(wú)明顯差異( P gt;0. 05) 。結(jié)論 COPD 大鼠膈肌組織內(nèi)泛素-蛋白酶體降解途徑被激活, 可能是引起蛋白降解的重要因素之一。
引用本文： 韓鋒鋒,徐衛(wèi)國(guó),羅勇,楊天蕓,陳迎春,張悅. COPD大鼠膈肌組織泛素-蛋白酶體途徑的研究. 中國(guó)呼吸與危重監(jiān)護(hù)雜志, 2010, 9(2): 141-144. doi: 復(fù)制
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| 3. | 李紅梅, 崔德健, 佟欣, 等. 熏香煙加氣管注內(nèi)毒素和單純熏香煙法建立大鼠COPD 模型. 中國(guó)病理生理雜志, 2002, 18: 808-812. |
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| 6. | Laaban JP. Nutrition and chronic respiratory failure. Ann Inter Med,2000, 151: 542-548. |
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| 12. | Levine S, Nguyen T, Kaiser LR, et al. Human diaphragm remodeling associated with chronic obstructive pulmonary disease: clinical implications. Am J Respir Crit Care Med, 2003, 168: 706 -713. |
| 13. | 孫麗華, 歐陽(yáng)曉平, 谷偉, 等. 胰島素樣生長(zhǎng)因子對(duì)COPD 大鼠膈肌凋亡的保護(hù)作用及對(duì)肺功能的影響. 中國(guó)呼吸與危重監(jiān)護(hù)雜志, 2009 , 8: 441-445 . |
| 14. | 楊天蕓, 徐衛(wèi)國(guó), 羅勇, 等. 慢性阻塞性肺病大鼠骨骼肌蛋白降解的變化. 上海交通大學(xué)學(xué)報(bào)( 醫(yī)學(xué)版) , 2008, 28 : 525-527. |
| 15. | 韓鋒鋒, 羅勇, 徐衛(wèi)國(guó). 慢性阻塞性肺疾病大鼠骨骼肌組織泛素系統(tǒng)基因及蛋白表達(dá)變化的研究. 上海醫(yī)學(xué), 2008; 31: 571 -574. |
| 16. | Taillandier D, Combaret L, Pouch MN, et al. The role of ubiquitinproteasome-dependent proteolysis in the remodelling of skeletal muscle. Proc Nutr Soc, 2004, 63 : 357-361. |
| 17. | McKinnell IW, Rudnicki MA. Molecular mechanisms of muscle atrophy. Cell, 2004, 119: 907-910. |
| 18. | Kornitzer D, Ciechanover A. Modes of regulation of ubiquitinmediated protein degradation. J Cell Physiol, 2000 , 182: 1-11. |
- 1. 王增禮. 更新對(duì)慢性阻塞性肺疾病認(rèn)識(shí)的觀念. 中國(guó)呼吸與危重監(jiān)護(hù)雜志, 2007 , 6: 401-402 .
- 2. Wray CJ, Sun X, Gang GJ, et al. Dantrolene downregulates the gene expression and activity of the ubiquitin-proteasome proteolytic pathway in septic skeletal muscle. J Surg Res, 2002 , 104: 82-87 .
- 3. 李紅梅, 崔德健, 佟欣, 等. 熏香煙加氣管注內(nèi)毒素和單純熏香煙法建立大鼠COPD 模型. 中國(guó)病理生理雜志, 2002, 18: 808-812.
- 4. Ausubel MF, Brent R, Konston ER, et al. 馬學(xué)軍, 舒躍龍, 譯校. 精編分子生物學(xué)實(shí)驗(yàn)指南. 第4 版. 北京: 科學(xué)出版社,2005,378-416.
- 5. Ezzell L, Jensen GI. Malnutrition in chronic obstructive pulmonary disease. Am J Clin Nutr, 2000, 72: 1415-1416.
- 6. Laaban JP. Nutrition and chronic respiratory failure. Ann Inter Med,2000, 151: 542-548.
- 7. Jagoe RT, Engelen MP. Muscle wasting and changes in muscle protein metabolism in chronic obstructive pulmonary disease. Eur Respir J, 2003, 46 : 52s-63 s.
- 8. Rutten EP, Franssen FM, Engelen MP, et al. Greater whole-body myofibrillar protein breakdown incachectic patients with chronic obstructive pulmonary disease. AmJ Clin Nutr,2006,83( 4) :829-834.
- 9. Laghi F, Tobin MJ. Disorders of the respiratory muscles. Am J Respir Crit Care Med, 2003, 168: 10 -48.
- 10. Ottenheijm CAC, Heunks LMA, Hafmans T, et al. Titin and diaphragmdysfunction in chronic obstructive pulmonary disease. Am J Respir Crit Care Med, 2006 , 173: 527-534 .
- 11. Barreiro E, de la Puente B, Minguella J, et al. Oxidative stress and respiratory muscle dysfunction in severe chronic obstructive pulmonary disease. Am J Respir Crit Care Med,2005, 171:1116-1124.
- 12. Levine S, Nguyen T, Kaiser LR, et al. Human diaphragm remodeling associated with chronic obstructive pulmonary disease: clinical implications. Am J Respir Crit Care Med, 2003, 168: 706 -713.
- 13. 孫麗華, 歐陽(yáng)曉平, 谷偉, 等. 胰島素樣生長(zhǎng)因子對(duì)COPD 大鼠膈肌凋亡的保護(hù)作用及對(duì)肺功能的影響. 中國(guó)呼吸與危重監(jiān)護(hù)雜志, 2009 , 8: 441-445 .
- 14. 楊天蕓, 徐衛(wèi)國(guó), 羅勇, 等. 慢性阻塞性肺病大鼠骨骼肌蛋白降解的變化. 上海交通大學(xué)學(xué)報(bào)( 醫(yī)學(xué)版) , 2008, 28 : 525-527.
- 15. 韓鋒鋒, 羅勇, 徐衛(wèi)國(guó). 慢性阻塞性肺疾病大鼠骨骼肌組織泛素系統(tǒng)基因及蛋白表達(dá)變化的研究. 上海醫(yī)學(xué), 2008; 31: 571 -574.
- 16. Taillandier D, Combaret L, Pouch MN, et al. The role of ubiquitinproteasome-dependent proteolysis in the remodelling of skeletal muscle. Proc Nutr Soc, 2004, 63 : 357-361.
- 17. McKinnell IW, Rudnicki MA. Molecular mechanisms of muscle atrophy. Cell, 2004, 119: 907-910.
- 18. Kornitzer D, Ciechanover A. Modes of regulation of ubiquitinmediated protein degradation. J Cell Physiol, 2000 , 182: 1-11.