• 山西醫(yī)科大學(xué)第一醫(yī)院呼吸科( 山西太原 030001)通訊作者: 許建英, E-mail: xujyty@ tom. com;

目的  通過研究不同吸煙量對大鼠血清瘦素、脂聯(lián)素、IL-6 及C 反應(yīng)蛋白( CRP) 水平的影響, 探討瘦素和脂聯(lián)素等炎癥因子在吸煙所致COPD 全身炎癥反應(yīng)中的作用。方法  雄性Wistar 大鼠30 只, 隨機(jī)分為大量吸煙組, 小量吸煙組和對照組, 每組各10 只。大量吸煙組每次吸煙10 支, 每天吸煙2 次, 每周吸煙6 d, 共吸煙20 周; 小量吸煙組每次吸煙5 支, 吸煙時(shí)間同前。采用雙抗體夾心酶聯(lián)免疫吸附法( ABC-ELISA) 分別檢測血清瘦素、脂聯(lián)素、IL-6 及CRP 水平。結(jié)果  大量吸煙組和小量吸煙組血清瘦素和脂聯(lián)素水平均較對照組降低( P 均 lt;0. 05) , 大量吸煙組與小量吸煙組差異無統(tǒng)計(jì)學(xué)意義( P  gt;0. 05) 。大量吸煙組和小量吸煙組血清IL-6 和CRP 水平均較對照組升高( P 均 lt;0. 05) , 大量吸煙組較小量吸煙組水平高( P 均 lt;0. 05) 。結(jié)論  吸煙可使大鼠血清瘦素與脂聯(lián)素水平下降, IL-6 與CRP水平升高, 提示瘦素、脂聯(lián)素等炎癥因子可能參與吸煙所致COPD 的全身炎癥反應(yīng)。

引用本文： 白學(xué)禮,許建英. 吸煙對大鼠血清瘦素、脂聯(lián)素、IL-6 和C 反應(yīng)蛋白的影響. 中國呼吸與危重監(jiān)護(hù)雜志, 2010, 9(3): 298-300. doi: 復(fù)制

版權(quán)信息： ?四川大學(xué)華西醫(yī)院華西期刊社《中國呼吸與危重監(jiān)護(hù)雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

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2. Sin DD, Anthonisen NR, Soriano JB, et al. Mortality in COPD: Role of comorbidities. Eur Respir J, 2006, 28: 1245-1257.
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4. Kirdar S, Serter M, Ceylan E, et al. Adiponectin as a biomarker of systemic inflammatory response in smoker patients with stable and exacerbation phases of chronic obstructive pulmonary disease. Scand.J Clin Lab Invest, 2009, 69: 219 -224.
5. 許三林, 吳人亮, 陳春蓮, 等. 上皮鈣黏附素在吸煙小鼠呼吸道上皮損傷修復(fù)中表達(dá)的研究. 中華結(jié)核和呼吸雜志,1999, 22:417-419.
6. Chen H, Hansen MJ, Jones JE, et al. Long-term cigarette smoke exposure increases uncoupling protein expression but reduces energy intake. Brain Res, 2008, 1228: 81-88.
7. Hotta Y, Yatsuya H, Toyoshima H, et al. Low leptin but high insulin resistance of smokers in Japanese men. Diabetes Res Clin Pract,2008, 81: 358-364 .
8. Sull JW, Kim HJ, Yun JE, et al. Serumadiponectin is associated with smoking status in healthy Korean men. Endocr J, 2009, 56: 73-78.
9. Kirchgessner TG, Uysal KT, Wiesbrock SM, et al. Tumor necrosis factor-alpha contributes to obesity-related hyperleptinemia by regulating leptin release from adipocytes. J Clin Invest, 1997, 100 :2777-2782.
10. Trujillo ME, Sullivan S, Harten I, et al. Interleukin-6 regulates human adipose tissue lipid metabolism and leptin production in vitro. J Clin Endocrinol Metab, 2004, 89 : 5577 -5582 .
11. Fantuzzi G. Adipose tissue, adipokines, and inflammation. J Allergy Clin Immunol, 2005, 115: 911 -919.
12. Gettys TW, Harkness PJ, Waston PM. The β3-aderenergic receptor inhibits insulin-stinulated leptin secretion from isolated rat adipocytes. Endocrinology, 1996, 137: 4054-4057.
13. Bruun JM, Lihn AS, Verdich C, et al. Regulation of adiponectin by adipose tissue-derived cytokines: in vivo and in vitro investigations in humans. Am J Physiol Endocrinol Metab, 2003, 285 : E527-533.
14. Iwashima Y, Katauya T, Ishikawa K, et al. Association of hypoadiponectinemia with smoking habit in men. Hypertension,2005, 45: 1094-1100 .
15. Pepys MB, Hirschfield GM. C-reactive protein: a critical update. J Clin Invest, 2003 , 111: 1805-1812.
  1. 1. van Weel C, Schellevis FG. Comorbidity and guidelines: conflicting interests. Lancet, 2006 , 367: 550-551.
  2. 2. Sin DD, Anthonisen NR, Soriano JB, et al. Mortality in COPD: Role of comorbidities. Eur Respir J, 2006, 28: 1245-1257.
  3. 3. Schols AM, Creutzerg EC, Buurman WA, et al. Plasma leptin is related to proinflammatory status and dietary intake in patients with chronic obstructive pulmonary disease. Am J Respir Crit Care Med,1999, 160: 1220-1226.
  4. 4. Kirdar S, Serter M, Ceylan E, et al. Adiponectin as a biomarker of systemic inflammatory response in smoker patients with stable and exacerbation phases of chronic obstructive pulmonary disease. Scand.J Clin Lab Invest, 2009, 69: 219 -224.
  5. 5. 許三林, 吳人亮, 陳春蓮, 等. 上皮鈣黏附素在吸煙小鼠呼吸道上皮損傷修復(fù)中表達(dá)的研究. 中華結(jié)核和呼吸雜志,1999, 22:417-419.
  6. 6. Chen H, Hansen MJ, Jones JE, et al. Long-term cigarette smoke exposure increases uncoupling protein expression but reduces energy intake. Brain Res, 2008, 1228: 81-88.
  7. 7. Hotta Y, Yatsuya H, Toyoshima H, et al. Low leptin but high insulin resistance of smokers in Japanese men. Diabetes Res Clin Pract,2008, 81: 358-364 .
  8. 8. Sull JW, Kim HJ, Yun JE, et al. Serumadiponectin is associated with smoking status in healthy Korean men. Endocr J, 2009, 56: 73-78.
  9. 9. Kirchgessner TG, Uysal KT, Wiesbrock SM, et al. Tumor necrosis factor-alpha contributes to obesity-related hyperleptinemia by regulating leptin release from adipocytes. J Clin Invest, 1997, 100 :2777-2782.
  10. 10. Trujillo ME, Sullivan S, Harten I, et al. Interleukin-6 regulates human adipose tissue lipid metabolism and leptin production in vitro. J Clin Endocrinol Metab, 2004, 89 : 5577 -5582 .
  11. 11. Fantuzzi G. Adipose tissue, adipokines, and inflammation. J Allergy Clin Immunol, 2005, 115: 911 -919.
  12. 12. Gettys TW, Harkness PJ, Waston PM. The β3-aderenergic receptor inhibits insulin-stinulated leptin secretion from isolated rat adipocytes. Endocrinology, 1996, 137: 4054-4057.
  13. 13. Bruun JM, Lihn AS, Verdich C, et al. Regulation of adiponectin by adipose tissue-derived cytokines: in vivo and in vitro investigations in humans. Am J Physiol Endocrinol Metab, 2003, 285 : E527-533.
  14. 14. Iwashima Y, Katauya T, Ishikawa K, et al. Association of hypoadiponectinemia with smoking habit in men. Hypertension,2005, 45: 1094-1100 .
  15. 15. Pepys MB, Hirschfield GM. C-reactive protein: a critical update. J Clin Invest, 2003 , 111: 1805-1812.