• 四川大學華西醫(yī)院泌尿外科（成都，610041）;

【摘 要】 目的 綜述壓力性尿失禁（stress urinary incontinence，SUI） 細胞治療的研究現(xiàn)狀及臨床應(yīng)用進展。 方
法 廣泛查閱近年來有關(guān)SUI 細胞治療的文獻并進行綜述。 結(jié)果 軟骨細胞和成肌細胞因不具有或僅有較差的再生
修復(fù)能力，限制了二者的臨床應(yīng)用；干細胞因具有較強的自我更新和再生修復(fù)能力被認為是治療SUI 的理想種子細胞，已
在動物實驗及初步臨床研究中取得了令人振奮的成果，為SUI 細胞治療可行性奠定了理論基礎(chǔ)。 結(jié)論 細胞治療尤其
是干細胞治療為SUI 提供了一種新的治療手段，其具體作用機制還有待進一步研究。

引用本文： 員海超,柳良仁,魏強,韓平. 壓力性尿失禁的細胞治療. 中國修復(fù)重建外科雜志, 2012, 26(2): 182-185. doi: 復(fù)制

1. Peggy N, Brubaker L. Urinary incontinence in women. Lancet, 2006, 367(9504): 57-67.
2. Hampel C, Wienhold D, Benken N, et al. Prevalence and natural history of female incontinence. Eur Urol, 1997, 32 Suppl 2: 3-12.
3. Bump RC, Norton PA. Epidemiology and natural history of pelvic fioor dysfunction. Obstet Gynecol Clin North Am, 1998, 25(4): 723-746.
4. Strasser H, Berjukow S, Marksteiner R, et al. Stem cell therapy for urinary stress incontinence. Exp Gerontol, 2004, 39(9): 1259-1265．.
5. 韓平, 解慧琪, 羅靜聰, 等. 細胞治療臨床應(yīng)用新進展. 中國修復(fù)重建外科雜志, 2008, 22(2): 221-227.
6. Bhatia NN, Ho MH. Stem cell therapy for urinary incontinence and pelvic floor disorders: a novel approach. Curr Opin Obstet Gynecol, 2004, 16(5): 397-398．.
7. Corcos J, Loutochin O, Campeau L, et al. Bone marrow mesenchymal stromal cell therapy for External urethral sphincter restoration in a rat model of stress urinary incontinence. Neurourol Urodyn, 2011, 30(3): 447-455.
8. Kim SO, Na HS, Kwon D, et al. Bone-marrow-derived Mese nc hymal stem cell transplantation enhances closing pressure and leak point pressure in a female urinary incontinence rat model. Urol Int, 2011, 86(1): 110-116.
9. Kinebuchi Y, Aizawa N, Imamura T, et al. Autologous bone-marrow-derived mesenchymal stem cell transplantation into injured rat urethral sphincter. Int J Urol, 2010, 17(4): 359-368.
10. Imamura T, Ishizuka O, Kinebuchi Y, et al. Implantation of autologous bone-marrow-derived cells Reconstructs functional urethral sphincters in rabbits. Tissue Eng Part a, 2011, 17(7-8): 1069-1081.
11. Strasser H, Marksteiner R, Margreiter E, et al. Transurethral ultrasound guided injection of clonally cultured autologous myoblasts and fibroblasts: experimental results. J Urol, 2003, 169: 39.
12. Lee JY, Cannon TW, Pruchnic R, et al. The effects of periurethral muscle-derived stem cell injection on leak point pressure in a rat model of stress urinary incontinence. Int Urogynecol J Pelvic Floor Dysfunct, 2003, 14(1): 31-37.
13. Gimble JM. Adipose tissue-derived therapeutics. Expert Opin Biol Ther, 2003, 3(5): 705-713.
14. Wu G, Zheng X, Jiang Z, et al. Induced differentiation of adipose-derived stromal cells into myoblasts. J Huazhong Univ Sci Technol, 2010, 30(3): 285-290.
15. Forraz N, Mcguckin CP. The umbilical cord: a rich and ethical stem cell source to advance regenerative medicine. Cell Prolif, 2011, 44(Suppl 1): 60-69.
16. Chermansky CJ, Tarin T, Kwon DD, et al. Intraurethral muscle-derived cell injections increase leak point pressure in a rat model of intrinsic sphincter deficiency. Urology, 2004, 63(4): 780-785.
17. Lim JJ, Jang JB, Kim JY, et al. Human umbilical cord blood mononuclear cell transplantation in rats with intrinsic sphin cter deficiency. J Korean Med Sci, 2010, 25(5): 663-670.
18. Carr LK, Steele D, Steele S, et al. 1-year follow-up of autologous muscle-derived stem cell injection pilot study to treat stress urinary incontinence. Int Urogynecol J Pelvic Floor Dysfunct, 2008, 19(6): 881-883.
19. Lee CN, Jang JB, Kim JY, et al. Human cord blood stem cell therapy for treatment of stress urinary incontinence. J Korean Med Sci, 2010, 25(6): 813-816.
20. Atala A, Cima LG, Kim W, et al. Injectable alginate seeded with chondrocytes as a potential treatment for vesicoureteral reflux. J Urol, 1993, 150(2 Pt 2): 745-747．.
21. Atala A, Kim W, Paige KT, et al. Endoscopic treatment of vesicoureteral reflux with a chondrocyte-alginate suspension. J Urol, 1994, 152(2 Pt 2): 641-643.
22. Bent AE, Tutrone RT, McLennan MT, et al. Treatment of intrinsic sphincter deficiency using autologous ear chond rocytes as a bulking agent. Neurourol Urodyn, 2001, 20(2): 157-165.
23. Vandenburgh H, Del TM, Shansky J, et al. Tissue-engineered skeletal muscle organoids for reversible gene therapy. Hum Gene Ther, 1996, 7(17): 2195-2200.
24. Chancellor MB, Yokoyama T, Tirney S, et al. Preliminary results of myoblast injection into the urethra and bladder wall: a possible method for the treatment of stress urinary incontin ence and impaired detrusor contractility. Neurourol Urodyn, 2000, 19(3): 279-287.
25. Wakitani S, Saito T, Caplan AI. Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine. Muscle Nerve, 1995, 18(12): 1417-1426.
26. Strasser H, Berjukow S, Marksteiner R, et al. Stem cell therapy for urinary stress incontinence. Exp Gerontol, 2004, 39(9): 1259-1265.
27. Fu Q, Song XF, G, Liao GL, et al. Myoblasts differentiated from adipose-derived stem cells to treat stress urinary incontinence. Urology, 2010, 75(2): 718-723.
28. Lee OK, Kuo TK, Chen WM, et al. Isolation of multipotent mesenchymal stem cells from umbilical cord blood. Blood, 2004, 103(5): 1669-1675.
29. Sarugaser R, Lickorish D, Baksh D, et al. Human umbilical cord perivascular(hucpv)cells: a source of mesenchymal progenitors. Stem Cells, 2005, 23(2): 220-229.
30. Kaviani A, Perry TE, Dzakovic A, et al. The amniotic fluid as a source of cells for fetal tissue engineering. J Pediatr Surg, 2001, 36(11): 1662-1665.
31. Bilic G, Zeisberger SM, Mallik AS, et al. Comparative characterization of cultured human term amnion epithelial and mesenchymal stromal cells for application in cell therapy. Cell Transplant, 2008, 17(8): 955-968.
32. Portmann-Lanz PC, Andreina S, Huber A, et al. Placental mesenchymal stem cells as potential autologous graft for pre-and perinatal neuroregeneration. Am J Obstet Gynecol, 2006, 194(3): 664-673.
  1. 1. Peggy N, Brubaker L. Urinary incontinence in women. Lancet, 2006, 367(9504): 57-67.
  2. 2. Hampel C, Wienhold D, Benken N, et al. Prevalence and natural history of female incontinence. Eur Urol, 1997, 32 Suppl 2: 3-12.
  3. 3. Bump RC, Norton PA. Epidemiology and natural history of pelvic fioor dysfunction. Obstet Gynecol Clin North Am, 1998, 25(4): 723-746.
  4. 4. Strasser H, Berjukow S, Marksteiner R, et al. Stem cell therapy for urinary stress incontinence. Exp Gerontol, 2004, 39(9): 1259-1265．.
  5. 5. 韓平, 解慧琪, 羅靜聰, 等. 細胞治療臨床應(yīng)用新進展. 中國修復(fù)重建外科雜志, 2008, 22(2): 221-227.
  6. 6. Bhatia NN, Ho MH. Stem cell therapy for urinary incontinence and pelvic floor disorders: a novel approach. Curr Opin Obstet Gynecol, 2004, 16(5): 397-398．.
  7. 7. Corcos J, Loutochin O, Campeau L, et al. Bone marrow mesenchymal stromal cell therapy for External urethral sphincter restoration in a rat model of stress urinary incontinence. Neurourol Urodyn, 2011, 30(3): 447-455.
  8. 8. Kim SO, Na HS, Kwon D, et al. Bone-marrow-derived Mese nc hymal stem cell transplantation enhances closing pressure and leak point pressure in a female urinary incontinence rat model. Urol Int, 2011, 86(1): 110-116.
  9. 9. Kinebuchi Y, Aizawa N, Imamura T, et al. Autologous bone-marrow-derived mesenchymal stem cell transplantation into injured rat urethral sphincter. Int J Urol, 2010, 17(4): 359-368.
  10. 10. Imamura T, Ishizuka O, Kinebuchi Y, et al. Implantation of autologous bone-marrow-derived cells Reconstructs functional urethral sphincters in rabbits. Tissue Eng Part a, 2011, 17(7-8): 1069-1081.
  11. 11. Strasser H, Marksteiner R, Margreiter E, et al. Transurethral ultrasound guided injection of clonally cultured autologous myoblasts and fibroblasts: experimental results. J Urol, 2003, 169: 39.
  12. 12. Lee JY, Cannon TW, Pruchnic R, et al. The effects of periurethral muscle-derived stem cell injection on leak point pressure in a rat model of stress urinary incontinence. Int Urogynecol J Pelvic Floor Dysfunct, 2003, 14(1): 31-37.
  13. 13. Gimble JM. Adipose tissue-derived therapeutics. Expert Opin Biol Ther, 2003, 3(5): 705-713.
  14. 14. Wu G, Zheng X, Jiang Z, et al. Induced differentiation of adipose-derived stromal cells into myoblasts. J Huazhong Univ Sci Technol, 2010, 30(3): 285-290.
  15. 15. Forraz N, Mcguckin CP. The umbilical cord: a rich and ethical stem cell source to advance regenerative medicine. Cell Prolif, 2011, 44(Suppl 1): 60-69.
  16. 16. Chermansky CJ, Tarin T, Kwon DD, et al. Intraurethral muscle-derived cell injections increase leak point pressure in a rat model of intrinsic sphincter deficiency. Urology, 2004, 63(4): 780-785.
  17. 17. Lim JJ, Jang JB, Kim JY, et al. Human umbilical cord blood mononuclear cell transplantation in rats with intrinsic sphin cter deficiency. J Korean Med Sci, 2010, 25(5): 663-670.
  18. 18. Carr LK, Steele D, Steele S, et al. 1-year follow-up of autologous muscle-derived stem cell injection pilot study to treat stress urinary incontinence. Int Urogynecol J Pelvic Floor Dysfunct, 2008, 19(6): 881-883.
  19. 19. Lee CN, Jang JB, Kim JY, et al. Human cord blood stem cell therapy for treatment of stress urinary incontinence. J Korean Med Sci, 2010, 25(6): 813-816.
  20. 20. Atala A, Cima LG, Kim W, et al. Injectable alginate seeded with chondrocytes as a potential treatment for vesicoureteral reflux. J Urol, 1993, 150(2 Pt 2): 745-747．.
  21. 21. Atala A, Kim W, Paige KT, et al. Endoscopic treatment of vesicoureteral reflux with a chondrocyte-alginate suspension. J Urol, 1994, 152(2 Pt 2): 641-643.
  22. 22. Bent AE, Tutrone RT, McLennan MT, et al. Treatment of intrinsic sphincter deficiency using autologous ear chond rocytes as a bulking agent. Neurourol Urodyn, 2001, 20(2): 157-165.
  23. 23. Vandenburgh H, Del TM, Shansky J, et al. Tissue-engineered skeletal muscle organoids for reversible gene therapy. Hum Gene Ther, 1996, 7(17): 2195-2200.
  24. 24. Chancellor MB, Yokoyama T, Tirney S, et al. Preliminary results of myoblast injection into the urethra and bladder wall: a possible method for the treatment of stress urinary incontin ence and impaired detrusor contractility. Neurourol Urodyn, 2000, 19(3): 279-287.
  25. 25. Wakitani S, Saito T, Caplan AI. Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine. Muscle Nerve, 1995, 18(12): 1417-1426.
  26. 26. Strasser H, Berjukow S, Marksteiner R, et al. Stem cell therapy for urinary stress incontinence. Exp Gerontol, 2004, 39(9): 1259-1265.
  27. 27. Fu Q, Song XF, G, Liao GL, et al. Myoblasts differentiated from adipose-derived stem cells to treat stress urinary incontinence. Urology, 2010, 75(2): 718-723.
  28. 28. Lee OK, Kuo TK, Chen WM, et al. Isolation of multipotent mesenchymal stem cells from umbilical cord blood. Blood, 2004, 103(5): 1669-1675.
  29. 29. Sarugaser R, Lickorish D, Baksh D, et al. Human umbilical cord perivascular(hucpv)cells: a source of mesenchymal progenitors. Stem Cells, 2005, 23(2): 220-229.
  30. 30. Kaviani A, Perry TE, Dzakovic A, et al. The amniotic fluid as a source of cells for fetal tissue engineering. J Pediatr Surg, 2001, 36(11): 1662-1665.
  31. 31. Bilic G, Zeisberger SM, Mallik AS, et al. Comparative characterization of cultured human term amnion epithelial and mesenchymal stromal cells for application in cell therapy. Cell Transplant, 2008, 17(8): 955-968.
  32. 32. Portmann-Lanz PC, Andreina S, Huber A, et al. Placental mesenchymal stem cells as potential autologous graft for pre-and perinatal neuroregeneration. Am J Obstet Gynecol, 2006, 194(3): 664-673.