• 四川大學華西醫(yī)院腫瘤中心頭頸-乳腺腫瘤科（成都，610041）;

目的 　研究人喉表皮癌細胞系Hep-2中乳腺癌易感基因1（BRCAl）、P53結(jié)合蛋白1（53BP1）和DNA損傷檢測點介質(zhì)1（MDC1）的表達及臨床意義。 方法 　采用逆轉(zhuǎn)錄聚合酶鏈式反應(yīng)檢測BRCA1、53BP1、MDC1在喉癌細胞系Hep-2中mRNA的表達，同時用免疫印跡法檢測蛋白的表達。 結(jié)果 　在所檢測的人喉癌細胞系Hep-2中BACR1、53BP1、MDC1在基因與蛋白兩個水平均有表達。 結(jié)論 　BRCA1、53BP1、MDC1可能在喉癌的發(fā)生發(fā)展中有一定作用。

引用本文： 鄧慧娟,張佳惠,楊莉,李平. 乳腺癌易感基因1、P53結(jié)合蛋白1DNA損傷檢測點介質(zhì)1在人喉表皮癌細胞中的表達及臨床意義. 華西醫(yī)學, 2012, 27(11): 1641-1644. doi: 復(fù)制

1. 　Hall JM, Lee MK, Newman B, et al. Linkage of early-onset familial breast cancer to chromosome 17q21[J]. Science, 1990, 250(4988): 1684-1689.
2. 　Wilson KA, Stern DF. NFBD1/MDC1, 53BP1 and BRCA1 have both redundant and unique roles in the ATM pathway[J]. Cell Cycle, 2008, 7(22): 3584-3594.
3. 　Judith EC, Jennifer EQ, Caroline OM, et al. BRCA1 is both a prognostic and predictive biomarker of response to chemotherapy in sporadic epithelial ovarian cancer[J]. Gynecologic Oncology, 2011, 3(123): 492-498.
4. 　Squatrito M, Vanoli F, Schultz N, et al. 53BP1 is a haploinsufficient tumor suppressor and protects cells from radiation response in glioma[J].Cancer Res, 2012, 72(20): 5250-5260.
5. 　Patel AN, Goyal S, Wu H, et al. Mediator of DNA damage checkpoint protein 1 (MDC1) expression as a prognostic marker for nodal recurrence in early-stage breast cancer patients treated with breast-conserving surgery and radiation therapy[J]. Breast Cancer Res Treat, 2010, 126(3): 601-607.
6. 　Roossink F, Wieringa HW, Noordhuis MG, et al. The role of ATM and 53BP1 as predictive markers in cervical cancer[J]. Int J Cancer, 2012, 131(9): 2056-2066.
7. 　Su C, Zhou S, Zhang L, et al. ERCC1, RRM1 and BRCA1 mRNA expression levels and clinical outcome of advanced non-small cell lung cancer[J]. Med Oncol, 2011, 28(4): 1411-1417.
8. 　Castro E, Eeles R. The role of BRCA1 and BRCA2 in prostate cancer[J]. Asian J Androl, 2012, 14(3): 409-414.
9. 　Mullan PB, Quinn JE, Gilmore PM, et al. BRCA1 and GADD45 mediated G2/M cell cycle arrest in response to antimicrotubule agents[J]. Oncogene, 2001, 20(43): 6123-6131.
10. 　Yarden RI, Pardo-Reoyo S, Sgagias M, et al. BRCA1 regulates the G2/M checkpoint by activating Chk1 kinase upon DNA damage[J]. Nat Genet, 2002, 30(3): 285-289.
11. 　Stewart GS, Wang B, Bignell CR, et al. MDC1 is a mediator of the mammalian DNA damage checkpoint[J]. Nature, 2003, 421(6926): 961-966.
12. 　Peng A, Chen PL. NFBD1/Mdc1 mediates ATR-dependent DNA damage response[J]. Cancer Res, 2005, 65(4): 1158-1163.
13. 　Bartek J, Falck J, Lukas J. CHK2 kinase a busy messenger[J]. Nat Rev Mol Cell Biol, 2001, 2(12): 877-886.
14. 　Ward IM, Minn K, Jorda KG, et al. Accumulation of checkpoint protein 53BP1 at DNA breaks involves its binding to phosphorylated histone H2AX[J]. Biol Chem, 2003, 278(22): 19579-19582.
15. 　Scully R, Puget N. BRCA1 and BRCA2 in hereditary breast cancer[J]. Biochimie, 2002, 84(1): 95-102.
16. 　Leongamornlert D, Mahmud N, Tymrakiewicz M, et al. Germline BRCA1 mutations increase prostate cancer risk[J]. Br J Cancer, 2012, 106(10): 1697-1701.
17. 　Rosell R, Skrzypski M, Jassem E, et al. BRCA1: a novel prognostic factor in resected non-small-cell lung cancer[J]. PLoS One, 2007, 2(11): e1129.
18. 　魯小敏, 茅國新, 吉浩明. BRCA1在非小細胞肺癌中的表達及其與鉑類化療敏感性的關(guān)系[J]. 實用醫(yī)學雜志, 2010, 26(19): 3526-3528.
19. 　張慧文, 汪淼, 王彥蘇, 等. BRCA1基因?qū)Σ煌伟┘毎派渲委熋舾行缘挠绊慬J]. 蘇州大學學報(醫(yī)學版), 2011, 31(5): 698-701, 712.
20. 　Mosconi M, Giesen U, Langner F, et al. 53BP1 and MDC1 foci formation in HT-1080 cells for low- and high-LET microbeam irradiations[J]. Radiat Environ Biophys, 2011, 50(3): 345-352.
21. 　Yuan C, Bu Y, Wang C, et al. NFBD1/MDC1 is a protein of oncogenic potential in human cervical cancer[J]. Mol Cell Biochem, 2012, 359(1-2): 333-346.
22. 　Bu Y, Suenaga Y, Okoshi R, et al. NFBD1/MDC1 participates in the regulation of G2/M transition in mammalian cells[J]. Biochem Biophys Res Commun, 2010, 397(2): 157-162.
23. 　Lou Z, Chini CC, Minter-Dykhouse K, et al. Mediator of DNA damage checkpoint protein 1 regulates BRCA1 localization and phosphorylation in DNA damage checkpoint control[J]. Biol Chem, 2003, 278(16): 13599-13602.
  1. 1. 　Hall JM, Lee MK, Newman B, et al. Linkage of early-onset familial breast cancer to chromosome 17q21[J]. Science, 1990, 250(4988): 1684-1689.
  2. 2. 　Wilson KA, Stern DF. NFBD1/MDC1, 53BP1 and BRCA1 have both redundant and unique roles in the ATM pathway[J]. Cell Cycle, 2008, 7(22): 3584-3594.
  3. 3. 　Judith EC, Jennifer EQ, Caroline OM, et al. BRCA1 is both a prognostic and predictive biomarker of response to chemotherapy in sporadic epithelial ovarian cancer[J]. Gynecologic Oncology, 2011, 3(123): 492-498.
  4. 4. 　Squatrito M, Vanoli F, Schultz N, et al. 53BP1 is a haploinsufficient tumor suppressor and protects cells from radiation response in glioma[J].Cancer Res, 2012, 72(20): 5250-5260.
  5. 5. 　Patel AN, Goyal S, Wu H, et al. Mediator of DNA damage checkpoint protein 1 (MDC1) expression as a prognostic marker for nodal recurrence in early-stage breast cancer patients treated with breast-conserving surgery and radiation therapy[J]. Breast Cancer Res Treat, 2010, 126(3): 601-607.
  6. 6. 　Roossink F, Wieringa HW, Noordhuis MG, et al. The role of ATM and 53BP1 as predictive markers in cervical cancer[J]. Int J Cancer, 2012, 131(9): 2056-2066.
  7. 7. 　Su C, Zhou S, Zhang L, et al. ERCC1, RRM1 and BRCA1 mRNA expression levels and clinical outcome of advanced non-small cell lung cancer[J]. Med Oncol, 2011, 28(4): 1411-1417.
  8. 8. 　Castro E, Eeles R. The role of BRCA1 and BRCA2 in prostate cancer[J]. Asian J Androl, 2012, 14(3): 409-414.
  9. 9. 　Mullan PB, Quinn JE, Gilmore PM, et al. BRCA1 and GADD45 mediated G2/M cell cycle arrest in response to antimicrotubule agents[J]. Oncogene, 2001, 20(43): 6123-6131.
  10. 10. 　Yarden RI, Pardo-Reoyo S, Sgagias M, et al. BRCA1 regulates the G2/M checkpoint by activating Chk1 kinase upon DNA damage[J]. Nat Genet, 2002, 30(3): 285-289.
  11. 11. 　Stewart GS, Wang B, Bignell CR, et al. MDC1 is a mediator of the mammalian DNA damage checkpoint[J]. Nature, 2003, 421(6926): 961-966.
  12. 12. 　Peng A, Chen PL. NFBD1/Mdc1 mediates ATR-dependent DNA damage response[J]. Cancer Res, 2005, 65(4): 1158-1163.
  13. 13. 　Bartek J, Falck J, Lukas J. CHK2 kinase a busy messenger[J]. Nat Rev Mol Cell Biol, 2001, 2(12): 877-886.
  14. 14. 　Ward IM, Minn K, Jorda KG, et al. Accumulation of checkpoint protein 53BP1 at DNA breaks involves its binding to phosphorylated histone H2AX[J]. Biol Chem, 2003, 278(22): 19579-19582.
  15. 15. 　Scully R, Puget N. BRCA1 and BRCA2 in hereditary breast cancer[J]. Biochimie, 2002, 84(1): 95-102.
  16. 16. 　Leongamornlert D, Mahmud N, Tymrakiewicz M, et al. Germline BRCA1 mutations increase prostate cancer risk[J]. Br J Cancer, 2012, 106(10): 1697-1701.
  17. 17. 　Rosell R, Skrzypski M, Jassem E, et al. BRCA1: a novel prognostic factor in resected non-small-cell lung cancer[J]. PLoS One, 2007, 2(11): e1129.
  18. 18. 　魯小敏, 茅國新, 吉浩明. BRCA1在非小細胞肺癌中的表達及其與鉑類化療敏感性的關(guān)系[J]. 實用醫(yī)學雜志, 2010, 26(19): 3526-3528.
  19. 19. 　張慧文, 汪淼, 王彥蘇, 等. BRCA1基因?qū)Σ煌伟┘毎派渲委熋舾行缘挠绊慬J]. 蘇州大學學報(醫(yī)學版), 2011, 31(5): 698-701, 712.
  20. 20. 　Mosconi M, Giesen U, Langner F, et al. 53BP1 and MDC1 foci formation in HT-1080 cells for low- and high-LET microbeam irradiations[J]. Radiat Environ Biophys, 2011, 50(3): 345-352.
  21. 21. 　Yuan C, Bu Y, Wang C, et al. NFBD1/MDC1 is a protein of oncogenic potential in human cervical cancer[J]. Mol Cell Biochem, 2012, 359(1-2): 333-346.
  22. 22. 　Bu Y, Suenaga Y, Okoshi R, et al. NFBD1/MDC1 participates in the regulation of G2/M transition in mammalian cells[J]. Biochem Biophys Res Commun, 2010, 397(2): 157-162.
  23. 23. 　Lou Z, Chini CC, Minter-Dykhouse K, et al. Mediator of DNA damage checkpoint protein 1 regulates BRCA1 localization and phosphorylation in DNA damage checkpoint control[J]. Biol Chem, 2003, 278(16): 13599-13602.