目的 探討異氟醚通過抑制細(xì)胞間黏附分子（ICAM-1）表達(dá)參與減輕肝臟缺血-再灌注（IR）損傷的可能調(diào)節(jié)機制。 方法 32只雌性SD大鼠分為4組。A組大鼠行腹腔注射1%戊巴比妥鈉40 mg/kg麻醉，進(jìn)行手術(shù)但不阻斷入肝血流；B組1%戊巴比妥鈉麻醉后行部分肝臟IR；C組大鼠僅接受1.0 MAC異氟醚吸入麻醉，不阻斷血流；D組采用1.0 MAC異氟醚麻醉，建立肝臟IR模型。肝臟缺血60 min，再灌注3 h后取肝組織和血液標(biāo)本，檢測血清丙氨酸轉(zhuǎn)氨酶（ALT）和天冬門氨酸轉(zhuǎn)氨酶（AST）、肝組織ICAM-1和肝組織還原型谷胱甘肽（GSH）、脂質(zhì)過氧化物丙二醛（MDA）和超氧化物歧化酶（SOD）含量。 結(jié)果 與戊巴比妥鈉麻醉比較，采用異氟醚處理后明顯降低血清ALT和AST的水平，再灌注肝組織內(nèi)GSH、SOD含量明顯高于而MDA含量降低，同時抑制肝組織ICAM-1的表達(dá)。 結(jié)論 異氟醚麻醉能夠有效減輕肝臟IR損傷，抑制氧自由基的生成和釋放，具體機制可能與抑制ICAM-1表達(dá)致使細(xì)胞內(nèi)GSH含量增加密切相關(guān)。
引用本文： 徐廣民,蘭志勛. 異氟醚對大鼠肝臟缺血-再灌注損傷期氧自由基及細(xì)胞間黏附分子-1表達(dá)的影響. 華西醫(yī)學(xué), 2012, 27(11): 1660-1663. doi: 復(fù)制
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- 1. 徐廣民, 陶國才, 易斌, 等. 異氟醚對大鼠缺血-再灌注損傷肝臟細(xì)胞黏附分子-1表達(dá)及中性粒細(xì)胞浸潤的影響[J]. 臨床麻醉學(xué)雜志, 2010, 26(11): 1004-1006.
- 2. 黃施偉, 李士通, 王瑩恬, 等. 異氟醚預(yù)處理對大鼠肝血竇內(nèi)皮缺血再灌注損傷的影響[J]. 中華麻醉學(xué)雜志, 2007, 27(11): 347-351.
- 3. 王旭東, 盧雅立, 王飛, 等. 七氟烷和異氟醚對肝臟缺血再灌注損傷的影響[J]. 中山大學(xué)學(xué)報(醫(yī)學(xué)科學(xué)版), 2008, 29(1): 28-33.
- 4. Pattillo CB, Pardue S, Shen X, et al. ICAM-1 cytoplasmic tail regulates endothelial glutathione synthesis through a NOX4/PI3-kinase-dependent pathway[J]. Free Radic Biol Med, 2010, 49(6): 1119-1128.
- 5. Pruitt HM, Langston W, Kevil CG, et al. ICAM-1 cross-linking stimulates endothelial glutathione synthesis[J]. Antioxid Redox Signal, 2007, 9(1): 159-164.
- 6. Li L, Yan L, Wang Z, et al, Change of apoptotic status in the human colorectal adenoma-carcinoma sequences and its correlation with carcinogenesis and prognosis[J]. Chin Med J (Engl), 2000, 113(10): 886-888.
- 7. Tao KM, Yang LQ, Liu YT, et al. Volatile anesthetics might be more beneficial than propofol for postoperative liver function in cirrhotic patients receiving hepatectomy[J]. Med Hypotheses, 2010, 75(6): 555-557.
- 8. Lv X, Wang ZM, Huang SD, et al. Emulsified isoflurane preconditioning reduces lung injury induced by hepatic ischemia/reperfusion in rats[J]. Int J Med Sci, 2011, 8(5): 353-361.
- 9. Ko JS, Kim G, Shin YH, et al. The effects of desflurane and isoflurane on hepatic and renal functions after right hepatectomy in living donors[J]. Transplant Proc, 2012, 44(2): 442-444.
- 10. Kim M, Park SW, Kim M, et al. Isoflurane activates intestinal sphingosine kinase to protect against renal ischemia-reperfusion-induced liver and intestine injury[J]. Anesthesiology, 2011, 114(2): 363-373.
- 11. 王雨, 湯禮軍, 戴睿武, 等. p38MAPK信號轉(zhuǎn)導(dǎo)途徑對早期缺血再灌注期間離體肝臟ICAM1 mRNA表達(dá)的影響[J]. 華西醫(yī)學(xué), 2009, 24(8): 2087-2091.
- 12. 葛廷, 王小琦, 廖世奇, 等. 肝缺血再灌注損傷機制及與相關(guān)細(xì)胞因子關(guān)系的研究進(jìn)展[J]. 甘肅醫(yī)藥, 2010, 29(5): 499-503.
- 13. Xiong Y, Uys JD, Tew KD, et al. S-glutathionylation: from molecular mechanisms to health outcomes[J]. Antioxid Redox Signal, 2011, 15(1): 233-270.