• 1 新疆維吾爾自治區(qū)人民醫(yī)院（烏魯木齊，830001）; 2 新疆大學(xué)化學(xué)化工學(xué)院分析教研室;

目的　 對尿液特征組分與糖尿病早期腎損害的關(guān)系進行了初步探索。 方法 　對2011年12月－2012年5月間28例2型糖尿病組、33例2型糖尿病腎病組及26例健康對照組尿液中尿蛋白含量和幾種常見非蛋白氮物質(zhì)，包括肌酸、尿囊素、肌酐、尿酸和假尿嘧啶核苷的濃度進行測定，采用多種歸一化方法對數(shù)據(jù)進行對比分析，并通過t檢驗減少高效液相色譜測定的變量信息，保留P＜0.05的檢出峰進行主成分分析，獲得分類結(jié)果。 結(jié)果 　采用體積歸一化方法，發(fā)現(xiàn)健康對照組尿液中肌酸、尿囊素和尿酸的含量與2型糖尿病組和糖尿病腎病組相比，差異均有統(tǒng)計學(xué)意義（P＜0.05），2型糖尿病組尿液中尿蛋白的濃度與糖尿病腎病組相比，差異有統(tǒng)計學(xué)意義（P＜0.05）。 結(jié)論 　通過肌酸、尿囊素、尿酸和尿蛋白的聯(lián)合測定為腎臟損傷程度的監(jiān)測及療效觀察提供依據(jù)，為2型糖尿病患者腎功能損壞的早期預(yù)防與診斷進行初步判斷提供了新的方法。

引用本文： 徐世茹,陳銳,吳朝陽,馬玉花,封順. 尿液特征組分與糖尿病早期腎損害的關(guān)系初探. 華西醫(yī)學(xué), 2012, 27(10): 1505-1509. doi: 復(fù)制

1. 　Moreira ED Jr, Neves RC, Nunes ZO, et al. Glycemic control and its correlates in patients with diabetes in Venezuela： results from a nationwide survey[J]. Diabetes Res Clin Pract, 2010, 87(3): 407-414.
2. 　梁紅峰, 姚錦繡，譚國據(jù)，等. 血清光抑素C測定在糖尿病及高血壓腎病早期診斷的臨床意義[J]. 華西醫(yī)學(xué), 2012, 27(6): 845-847.
3. 　許茜芳, 姬秋和. 糖尿病腎病早期診斷相關(guān)指標的研究進展[J]. 陜西醫(yī)學(xué)雜志, 2010, 39(7): 880-882.
4. 　Caubet C, Lacroix C, Decramer S, et al . Advances in urinary proteome analysis and biomarker discovery in pediatric renal disease[J]. Pediatr Nephrol, 2010, 25(1): 27-35.
5. 　Lowry OH, Rosebrough NJ, Farr AL, et al . Protein measurement with the Folin phenol reagent[J]. J Biol Chem, 1951, 193(1): 265-275.
6. 　Carlsson N, Borde A, Wolfel S, et al . Quantification of protein concentration by the Bradford method in the presence of pharmaceutical polymers[J]. Anal Biochem, 2011, 411(1): 116-121.
7. 　彭長華，王昌富，李承彬，等. HPLC同時檢測血清和尿樣中肌酐、假尿苷、尿酸[J]. 色譜, 1998, 16(2): 146-148.
8. 　Grabowska I, Chudy M, Dybko A, et al . Determination of creatinine in clinical samples based on flow-through microsystem[J]. Anal Chim Acta, 2005, 540(1): 181-185.
9. 　Husková R, Chrastina P, Adam T, et al . Determination of creatinine in urine by tandem mass spectrometry[J]. Clin Chim Acta, 2004, 350(1-2): 99-106.
10. 　Liotta E, Gottardo R, Bonizzato L, et al . Rapid and direct determination of creatinine in urine using capillary zone electrophoresis[J]. Clin Chim Acta, 2009, 409(1-2): 52-55.
11. 　Zuo Y, Yang Y, Zhu Z, et al . Determination of uric acid and creatinine in human urine using hydrophilic interaction chromatography[J]. Talanta, 2011, 83(5): 1707-1710.
12. 　Shingfield KJ, Offer NW. Simultaneous determination of purine metabolites, creatinine and pseudouridine in ruminant urine by reversed-phase highperformance liquid chromatography[J]. J Chromatogr B Biomed Sci Appl, 1999, 723(1-2): 81-94.
13. 　Rasmuson T, Björk GR. Urinary excretion of pseudouridine and prognosis of patients with malignant lymphoma[J]. Acta Oncol, 1995, 34(1): 61-67.
14. 　Zuo YG, Wang C, Zhou J, et al . Simultaneous determination of creatinine and uric acid in human urine by high-performance liquid chromatography[J]. Anal Sci, 2008, 24(12): 1589-1592.
15. 　Alfazema LN, Howells S, Perrett D. Determination of allantoin in biofluids using micellar electrokinetic capillary chromatography[J]. J Chromatogr A, 1998, 817(1-2): 345-352.
16. 　Erasmus RT, Okesina B. Microalbuminuria in a clinic population of type 2 Melanesian diabetics: relationship to glycaemic control[J]. Cent Afr J Med, 1999, 45 (11): 291-294.
17. 　姚勤，張峻，何瑾，等. 高效液相色譜法測定人血漿中同型半胱氨酸濃度[J]. 華西醫(yī)學(xué), 2012, 27(6): 809-811.
  1. 1. 　Moreira ED Jr, Neves RC, Nunes ZO, et al. Glycemic control and its correlates in patients with diabetes in Venezuela： results from a nationwide survey[J]. Diabetes Res Clin Pract, 2010, 87(3): 407-414.
  2. 2. 　梁紅峰, 姚錦繡，譚國據(jù)，等. 血清光抑素C測定在糖尿病及高血壓腎病早期診斷的臨床意義[J]. 華西醫(yī)學(xué), 2012, 27(6): 845-847.
  3. 3. 　許茜芳, 姬秋和. 糖尿病腎病早期診斷相關(guān)指標的研究進展[J]. 陜西醫(yī)學(xué)雜志, 2010, 39(7): 880-882.
  4. 4. 　Caubet C, Lacroix C, Decramer S, et al . Advances in urinary proteome analysis and biomarker discovery in pediatric renal disease[J]. Pediatr Nephrol, 2010, 25(1): 27-35.
  5. 5. 　Lowry OH, Rosebrough NJ, Farr AL, et al . Protein measurement with the Folin phenol reagent[J]. J Biol Chem, 1951, 193(1): 265-275.
  6. 6. 　Carlsson N, Borde A, Wolfel S, et al . Quantification of protein concentration by the Bradford method in the presence of pharmaceutical polymers[J]. Anal Biochem, 2011, 411(1): 116-121.
  7. 7. 　彭長華，王昌富，李承彬，等. HPLC同時檢測血清和尿樣中肌酐、假尿苷、尿酸[J]. 色譜, 1998, 16(2): 146-148.
  8. 8. 　Grabowska I, Chudy M, Dybko A, et al . Determination of creatinine in clinical samples based on flow-through microsystem[J]. Anal Chim Acta, 2005, 540(1): 181-185.
  9. 9. 　Husková R, Chrastina P, Adam T, et al . Determination of creatinine in urine by tandem mass spectrometry[J]. Clin Chim Acta, 2004, 350(1-2): 99-106.
  10. 10. 　Liotta E, Gottardo R, Bonizzato L, et al . Rapid and direct determination of creatinine in urine using capillary zone electrophoresis[J]. Clin Chim Acta, 2009, 409(1-2): 52-55.
  11. 11. 　Zuo Y, Yang Y, Zhu Z, et al . Determination of uric acid and creatinine in human urine using hydrophilic interaction chromatography[J]. Talanta, 2011, 83(5): 1707-1710.
  12. 12. 　Shingfield KJ, Offer NW. Simultaneous determination of purine metabolites, creatinine and pseudouridine in ruminant urine by reversed-phase highperformance liquid chromatography[J]. J Chromatogr B Biomed Sci Appl, 1999, 723(1-2): 81-94.
  13. 13. 　Rasmuson T, Björk GR. Urinary excretion of pseudouridine and prognosis of patients with malignant lymphoma[J]. Acta Oncol, 1995, 34(1): 61-67.
  14. 14. 　Zuo YG, Wang C, Zhou J, et al . Simultaneous determination of creatinine and uric acid in human urine by high-performance liquid chromatography[J]. Anal Sci, 2008, 24(12): 1589-1592.
  15. 15. 　Alfazema LN, Howells S, Perrett D. Determination of allantoin in biofluids using micellar electrokinetic capillary chromatography[J]. J Chromatogr A, 1998, 817(1-2): 345-352.
  16. 16. 　Erasmus RT, Okesina B. Microalbuminuria in a clinic population of type 2 Melanesian diabetics: relationship to glycaemic control[J]. Cent Afr J Med, 1999, 45 (11): 291-294.
  17. 17. 　姚勤，張峻，何瑾，等. 高效液相色譜法測定人血漿中同型半胱氨酸濃度[J]. 華西醫(yī)學(xué), 2012, 27(6): 809-811.