• 四川大學(xué)華西醫(yī)院麻醉科（成都，610041）;

目的 　觀察電壓依賴(lài)性鈣通道是否作用于大鼠脊髓背角膠狀質(zhì)層（SG）神經(jīng)元大振幅微小興奮性突觸后電流的形成。 方法 　選用成年雄性Sprague-Dawley（SD）大鼠，2%～3%異氟烷麻醉后，分離其腰骶部的脊髓，然后切片。采用全細(xì)胞電壓鉗技術(shù)，玻璃微電極的電阻為4~6 MΩ，鉗制電壓為?70 mV，記錄膠狀質(zhì)層神經(jīng)元微小興奮性突觸后電流（mEPSC）電流。將電流信號(hào)用Axopatch 200來(lái)放大并儲(chǔ)存于電腦。對(duì)照組和用藥結(jié)束后，持續(xù)采樣mEPSC電流30 s。mEPSC電流的頻率和振幅用Clampfit 8.1進(jìn)行分析。 結(jié)果 　鉗制電壓為?70 mV時(shí)，所有SG神經(jīng)元均有自發(fā)性的EPSC。辣椒素增加mEPSC發(fā)生的頻率和波幅。鈷離子抑制辣椒素誘導(dǎo)的大振幅mEPSC。鈷離子抑制辣椒素誘導(dǎo)的mEPSC的平均振幅，而不抑制其發(fā)生頻率。 結(jié)論 　電壓依賴(lài)性鈣離子通道參與了辣椒素引起的痛覺(jué)形成。

引用本文： 黃福森,楊小娟,王儒蓉,吳超然. 電壓依賴(lài)性鈣通道參與大振幅微小興奮性突觸后電流形成的實(shí)驗(yàn)研究. 華西醫(yī)學(xué), 2012, 27(6): 905-909. doi: 復(fù)制

1. 　Caterina MJ, Schumacher MA, Tominaga M, et al. The capsaicin receptor: a heat-activated ion channel in the pain pathway[J]. Nature, 1997, 389(6653): 816-824.
2. 　Sugiura Y, Lee CL, Perl ER. Central projections of identified, unmyelinated (C) afferent fibers innervating mammalian skin[J]. Science, 1986, 234(4774):358-361.
3. 　Savidge JR, Ranasinghe SP, Rang HP. Comparison of intracellular calcium signals evoked by heat and capsaicin in cultured rat dorsalroot ganglion neurons and in a cell line expressing the rat vanilloid receptor, VR1[J]. Neuroscience, 2001, 102(1): 177-184.
4. 　St Pierre M, Reeh PW, Zimmermann K. Differential effects of TRPV channel block on polymodal activation of rat cutaneous nociceptors in vitro[J]. Exp Brain Res, 2009, 196(1): 31-44.
5. 　Chard PS, Bleakman D, Savidge JR, et al. Capsaicin-induced neurotoxicity in cultured dorsal root ganglion neurons: involvement of calcium-activated proteases[J]. Neuroscience, 1995, 65(4): 1099-1108.
6. 　Cholewinski A, Burgess GM, Bevan S: The role of calcium in capsaicin-induced desensitization in rat cultured dorsal root ganglion neurons[J]. Neuroscience, 1993, 55(4): 1015-1023.
7. 　Baba H, Kohno T, Okamoto M, et al. Muscarinic facilitation of GABA release in substantia gelatinosa of the rat spinal dorsal horn[J]. J Physiol , 1998, 508 (Pt 1): 83-93.
8. 　Yoshimura M, Nishi S: Blind patch-clamp recordings from substantia gelatinosa neurons in adult rat spinal cord slices: pharmacological properties of synaptic currents[J]. Neuroscience, 1993, 53(2): 519-526.
9. 　Holzer P. Capsaicin: cellular targets, mechanisms of action, and selectivity for thin sensory neurons[J]. Pharmacol Rev , 1991, 43(2): 143-201.
10. 　Zeilhofer HU, Kress M, Swandulla D. Fractional Ca2+ currents through capsaicin- and proton-activated ion channels in rat dorsal root ganglion neurones[J]. J Physiol , 1997, 503(1): 67-78.
11. 　Larkman A, Stratford K, Jack J. Quantal analysis of excitatory synaptic action and depression in hippocampal slices[J]. Nature , 1991, 350(6316): 344-347.
12. 　Yang K, Kumamoto E, Furue H, et al. Capsaicin facilitates excitatory but not inhibitory synaptic transmission in substantia gelatinosa of the rat spinal cord[J]. Neurosci Lett, 1998, 255(3): 135-138.
13. 　Ahluwalia J, Rang H, Nagy I. The putative role of vanilloid receptor-like protein-1 in mediating high threshold noxious heat-sensitivity in rat cultured primary sensory neurons[J]. Eur J Neurosci, 2002 , 16(8): 1483-1489.
14. 　Larkman AU, Jack JJ, Stratford KJ. Quantal analysis of excitatory synapses in rat hippocampal CA1 in vitro during low-frequencydepression[J]. J Physiol, 1997, 505 (Pt 2): 457-471.
15. 　Feldmeyer D, Radnikow G. Developmental alterations in the functional properties of excitatory neocortical synapses[J]. J Physiol, 2009, 587(Pt 9): 1889-1896.
16. 　Jiang CY, Fujita T, Yue HY, et al. Effect of resiniferatoxin on glutamatergic spontaneous excitatory synaptic transmission in substantia gelatinosa neurons of the adult rat spinal cord[J]. Neuroscience, 2009 , 164(4): 1833-1844.
  1. 1. 　Caterina MJ, Schumacher MA, Tominaga M, et al. The capsaicin receptor: a heat-activated ion channel in the pain pathway[J]. Nature, 1997, 389(6653): 816-824.
  2. 2. 　Sugiura Y, Lee CL, Perl ER. Central projections of identified, unmyelinated (C) afferent fibers innervating mammalian skin[J]. Science, 1986, 234(4774):358-361.
  3. 3. 　Savidge JR, Ranasinghe SP, Rang HP. Comparison of intracellular calcium signals evoked by heat and capsaicin in cultured rat dorsalroot ganglion neurons and in a cell line expressing the rat vanilloid receptor, VR1[J]. Neuroscience, 2001, 102(1): 177-184.
  4. 4. 　St Pierre M, Reeh PW, Zimmermann K. Differential effects of TRPV channel block on polymodal activation of rat cutaneous nociceptors in vitro[J]. Exp Brain Res, 2009, 196(1): 31-44.
  5. 5. 　Chard PS, Bleakman D, Savidge JR, et al. Capsaicin-induced neurotoxicity in cultured dorsal root ganglion neurons: involvement of calcium-activated proteases[J]. Neuroscience, 1995, 65(4): 1099-1108.
  6. 6. 　Cholewinski A, Burgess GM, Bevan S: The role of calcium in capsaicin-induced desensitization in rat cultured dorsal root ganglion neurons[J]. Neuroscience, 1993, 55(4): 1015-1023.
  7. 7. 　Baba H, Kohno T, Okamoto M, et al. Muscarinic facilitation of GABA release in substantia gelatinosa of the rat spinal dorsal horn[J]. J Physiol , 1998, 508 (Pt 1): 83-93.
  8. 8. 　Yoshimura M, Nishi S: Blind patch-clamp recordings from substantia gelatinosa neurons in adult rat spinal cord slices: pharmacological properties of synaptic currents[J]. Neuroscience, 1993, 53(2): 519-526.
  9. 9. 　Holzer P. Capsaicin: cellular targets, mechanisms of action, and selectivity for thin sensory neurons[J]. Pharmacol Rev , 1991, 43(2): 143-201.
  10. 10. 　Zeilhofer HU, Kress M, Swandulla D. Fractional Ca2+ currents through capsaicin- and proton-activated ion channels in rat dorsal root ganglion neurones[J]. J Physiol , 1997, 503(1): 67-78.
  11. 11. 　Larkman A, Stratford K, Jack J. Quantal analysis of excitatory synaptic action and depression in hippocampal slices[J]. Nature , 1991, 350(6316): 344-347.
  12. 12. 　Yang K, Kumamoto E, Furue H, et al. Capsaicin facilitates excitatory but not inhibitory synaptic transmission in substantia gelatinosa of the rat spinal cord[J]. Neurosci Lett, 1998, 255(3): 135-138.
  13. 13. 　Ahluwalia J, Rang H, Nagy I. The putative role of vanilloid receptor-like protein-1 in mediating high threshold noxious heat-sensitivity in rat cultured primary sensory neurons[J]. Eur J Neurosci, 2002 , 16(8): 1483-1489.
  14. 14. 　Larkman AU, Jack JJ, Stratford KJ. Quantal analysis of excitatory synapses in rat hippocampal CA1 in vitro during low-frequencydepression[J]. J Physiol, 1997, 505 (Pt 2): 457-471.
  15. 15. 　Feldmeyer D, Radnikow G. Developmental alterations in the functional properties of excitatory neocortical synapses[J]. J Physiol, 2009, 587(Pt 9): 1889-1896.
  16. 16. 　Jiang CY, Fujita T, Yue HY, et al. Effect of resiniferatoxin on glutamatergic spontaneous excitatory synaptic transmission in substantia gelatinosa neurons of the adult rat spinal cord[J]. Neuroscience, 2009 , 164(4): 1833-1844.