• 四川大學華西醫(yī)院（成都，610041） 1 麻醉科，2 麻醉學與危重病教研室;

【摘要】　目的　 探討辣椒素對不同年齡SD大鼠內臟感覺神經(jīng)元上辣椒素受體（TRPV1）介導的離子通道的影響。　方法　 急性分離7~9 d和21~23 d大鼠迷走神經(jīng)結狀神經(jīng)節(jié)神經(jīng)元，利用全細胞膜片鉗技術在分離的神經(jīng)元上記錄辣椒素激活TRPV1受體后通道電流的變化。　結果　 ①7~9 d和21~23 d大鼠內臟感覺神經(jīng)元的膜電容分別為（18.57±8.60）和（19.85±9.47） pF，（P gt;0.05）；②辣椒素能夠激活7~9 d和21~23 d大鼠內臟感覺神經(jīng)元上TRPV1并產(chǎn)生相似的內向電流，兩組產(chǎn)生的峰電流密度分別為（48.59±18.87）、（55.91±20.52） pA/pF（P gt;0.05）；③反復應用辣椒素使TRPV1受體發(fā)生失敏現(xiàn)象。　結論　 大鼠內臟感覺神經(jīng)元的TRPV1受體通道在出生后已經(jīng)發(fā)育成熟，且對辣椒素激活的通道電流有相似的變化。
【Abstract】　Objective　 To investigate the effects of capsaicin on transient receptor potential vanilloid 1 (TRPV1) receptor-mediated ion channel currents of visceral sensory neurons in different-aged Sprague-Dawley rats.　Methods　 We isolated the vagal nodose ganglion neurons of rats at an age of 7-9 days or 21-23 days acutely. With the whole cell patch clamp technique, we recorded the current changes of TRPV1 channels activated by capsaicin.　Results　 ① Membrane capacitances of the visceral sensory neurons were (18.57±8.60) and (19.85±9.47) pF in rats of 7-9 and 21-23 days, respectively (P gt;0.05). ② Capsaicin activated the TRPV1 channels and generated inward currents in all the rats; and the peak current densities of the rats of 7-9 days and 21-23 days were respectively (48.59±18.87) and (55.91±20.52) pA/pF (P gt;0.05). ③ Repeated applications of capsaicin produced a phenomenon of desensitization in TRPV1 channels.　Conclusion　 TRPV1 receptor channels of visceral sensory neurons in rats have matured after birth, and the current changes of TRPV1 channels activated by capsaicin are similar.

引用本文： 李紅旗,廖大清,王儒蓉. 辣椒素對不同年齡大鼠內臟感覺神經(jīng)元上辣椒素受體通道的影響. 華西醫(yī)學, 2011, 26(2): 161-164. doi: 復制

1. 　Helliwell RJA, McLatchie LM, Clarke M, et al. Capsaicin sensitivity is associated with the expression of the vanilloid (capsaicin) receptor (VR1) mRNA in adult rat sensory ganglia[J]. Neurosci Lett, 1998, 250(3): 177-180.
2. 　Wang RR, Xu FD. Postnatal development of right atrial injection of capsaicin-induced apneic response in rats[J]. J Appl Physiol, 2006, 101(1): 60-67.
3. 　孫明潔, 基鵬, 王儒蓉. 右心房注射前列腺素E2對新生大鼠呼吸的影響[J]. 華西醫(yī)學, 2009, 24(2): 368-370.
4. 　Lee LY, Lundberg JM. Capsazepine abolishes pulmonary chemore-flexes induced by capsaicin in anesthetized rats[J]. J Appl Physiol, 1994, 76(5): 1848-1855.
5. 　Xu F, Gu QH, Lee LY, et al. Acute hypoxia prolongs the apnea induced by right atrial injection of capsaicin[J]. J Appl Physiol, 2003, 94(4): 1446-1454.
6. 　Barbara M, Grzegorz K, Manfred G. Anandamide and methanandamide induce both vanilloid VR1 and cannabinoid CB1 receptor-mediated changes in heart rate and blood pressure in anaesthetized rats[J]. Arch Pharmacol, 2001, 364(6): 562-569.
7. 　Wang RR, Xu FD, Zhang JG, et al. Carotid sinus nerve is involved in cardiorespiratory responses to intracarotid injection of capsaicin in the rats[J]. J Appl Physiol, 2006, 100(1): 60-66.
8. 　Vellani V, Mapplebeck S, Moriondo A, et al. Protein kinase C activaton potentiates gating of the vanilloid receptor VR1 by capsaicin, proton, heat and anandamide[J]. J Physiol, 2001, 534(3): 8132-8251.
9. 　Pan XQ, Gonzalez JA, Chang S, et al. Experimental colitis triggers the release of substance P and calcitonin gene-related peptide in the urinary bladder via TRPV1 signaling pathways[J]. Exp Neurol, 2010, 225(2): 262-273.
10. 　Helliwell RJA, McLatchie LM, Clarke M, et al. Capsaicin sensitivity is associated with the expression of the vanilloid (capsaicin) receptor (VR1) mRNA in adult rat sensory ganglia. Neurosci Lett, 1998, 250(3): 177-180.
11. 　Wu ZZ, Chen SR, Pan HL. Transient receptor potential vanilloid type 1 activation down-regulates voltage-gated calcium channels through calcium-dependent calcineurin in sensory neurons[J]. J Biol Chem. 2005, 280(18): 18142-18151.
12. 　Rosenbaum T, Gordon-Shaag A, Munari M, et al. Ca2+/calmodulin modulates TRPV1 activation by capsaicin[J]. J Gen Physiol, 2004, 123(1): 53-62.
  1. 1. 　Helliwell RJA, McLatchie LM, Clarke M, et al. Capsaicin sensitivity is associated with the expression of the vanilloid (capsaicin) receptor (VR1) mRNA in adult rat sensory ganglia[J]. Neurosci Lett, 1998, 250(3): 177-180.
  2. 2. 　Wang RR, Xu FD. Postnatal development of right atrial injection of capsaicin-induced apneic response in rats[J]. J Appl Physiol, 2006, 101(1): 60-67.
  3. 3. 　孫明潔, 基鵬, 王儒蓉. 右心房注射前列腺素E2對新生大鼠呼吸的影響[J]. 華西醫(yī)學, 2009, 24(2): 368-370.
  4. 4. 　Lee LY, Lundberg JM. Capsazepine abolishes pulmonary chemore-flexes induced by capsaicin in anesthetized rats[J]. J Appl Physiol, 1994, 76(5): 1848-1855.
  5. 5. 　Xu F, Gu QH, Lee LY, et al. Acute hypoxia prolongs the apnea induced by right atrial injection of capsaicin[J]. J Appl Physiol, 2003, 94(4): 1446-1454.
  6. 6. 　Barbara M, Grzegorz K, Manfred G. Anandamide and methanandamide induce both vanilloid VR1 and cannabinoid CB1 receptor-mediated changes in heart rate and blood pressure in anaesthetized rats[J]. Arch Pharmacol, 2001, 364(6): 562-569.
  7. 7. 　Wang RR, Xu FD, Zhang JG, et al. Carotid sinus nerve is involved in cardiorespiratory responses to intracarotid injection of capsaicin in the rats[J]. J Appl Physiol, 2006, 100(1): 60-66.
  8. 8. 　Vellani V, Mapplebeck S, Moriondo A, et al. Protein kinase C activaton potentiates gating of the vanilloid receptor VR1 by capsaicin, proton, heat and anandamide[J]. J Physiol, 2001, 534(3): 8132-8251.
  9. 9. 　Pan XQ, Gonzalez JA, Chang S, et al. Experimental colitis triggers the release of substance P and calcitonin gene-related peptide in the urinary bladder via TRPV1 signaling pathways[J]. Exp Neurol, 2010, 225(2): 262-273.
  10. 10. 　Helliwell RJA, McLatchie LM, Clarke M, et al. Capsaicin sensitivity is associated with the expression of the vanilloid (capsaicin) receptor (VR1) mRNA in adult rat sensory ganglia. Neurosci Lett, 1998, 250(3): 177-180.
  11. 11. 　Wu ZZ, Chen SR, Pan HL. Transient receptor potential vanilloid type 1 activation down-regulates voltage-gated calcium channels through calcium-dependent calcineurin in sensory neurons[J]. J Biol Chem. 2005, 280(18): 18142-18151.
  12. 12. 　Rosenbaum T, Gordon-Shaag A, Munari M, et al. Ca2+/calmodulin modulates TRPV1 activation by capsaicin[J]. J Gen Physiol, 2004, 123(1): 53-62.