【摘要】 目的 觀察機(jī)械通氣對(duì)黏蛋白（mucin，MUC）-5AC表達(dá)的影響及復(fù)方川貝精片的干預(yù)作用。 方法 新西蘭兔25只，6個(gè)月齡，雄性；隨機(jī)分為對(duì)照組、機(jī)械通氣12 h組及復(fù)方川貝精片低、中、高劑量組。收集支氣管灌洗液，分別采用實(shí)時(shí)熒光定量聚合酶鏈?zhǔn)椒磻?yīng)法和酶聯(lián)免疫吸附試驗(yàn)檢測(cè)支氣管灌洗液中p38 MAPK mRNA，MUC-5AC蛋白和mRNA的表達(dá)。 結(jié)果 機(jī)械通氣能增強(qiáng)MUC-5AC的分泌（P lt;0.05）；加用復(fù)方川貝精片能降低機(jī)械通氣后MUC-5AC蛋白和mRNA的表達(dá)（P lt;0.05）；復(fù)方川貝精片中、高劑量組與低劑量組比較，能降低機(jī)械通氣后MUC-5AC蛋白和mRNA的表達(dá)（P lt;0.05）。 結(jié)論 機(jī)械通氣能促進(jìn)支氣管黏膜上皮細(xì)胞分泌MUC-5AC，復(fù)方川貝精片能抑制機(jī)械通氣所致MUC-5AC表達(dá)升高，其機(jī)制可能與其抑制p38 MAPK表達(dá)有關(guān)。
【Abstract】 Objective To observe the effect of mechanical ventilation by breathing machine on the expression of mucin (MUC-5AC) and the interfering effect of compound tablet of fritillary bulb. Methods New Zealand Rabbits were randomly divided into control group, twelve-hour mechanical ventilation group, and low, medium and high-dose compound tablet of fritillary bulb group. Contents of p38 MAPK mRNA, MUC-5AC mRNA and protein in bronchial irrigating solution were detected by realtime RT-PCR and ELISA methods. Results Mechanical ventilation could increase the expression of MUC-5AC in bronchial irrigating solution (P lt;0.05). Compound tablet of fritillary bulb could decrease the expression of MUC-5AC mRNA and protein after mechanical ventilation (P lt;0.05). Compared with low-dose compound tablet of fritillary bulb group, the expression of MUC-5AC mRNA and protein was lower for the high and medium-dose groups (P lt;0.05). Conclusions Mechanical ventilation can promote the expression of MUC-5AC in bronchial endothelial cells, which can be suppressed by compound tablet of fritillary bulb. This may be due to the suppression effect of p38 MAPK expression.
引用本文： 董瓊,鄧安彥,王波,陳紹平,蒲青,陳艷. 機(jī)械通氣對(duì)黏蛋白-5AC表達(dá)的影響及復(fù)方川貝精片的干預(yù)作用. 華西醫(yī)學(xué), 2011, 26(6): 871-873. doi: 復(fù)制
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| 2. | O’Reilly M, Hooper SB, Allison BJ, et al. Persistent bronchiolar remodeling following brief ventilation of the very immature ovine lung[J]. Am J Physiol Lung Cell Mol Physiol, 2009, 297(5): 992-1001. |
| 3. | Durbin CG Jr, Perkins MP. Should tracheostomy be performed as early as 72 hours in patients requiring prolonged mechanical ventilation [J]. Respir Care, 2010, 55(1): 76-87. |
| 4. | EI Ferzli GT, Philips JB 3rd, Bulger A, et al. A pumpless lung assist device reduces mechanical ventilation-induced lung injury in juvenile piglets[J]. Pediatr Res, 2009, 66(6): 671-676. |
| 5. | 毛翎, 白春學(xué), 張敏, 等. 表皮生長(zhǎng)因子受體和黏蛋白在慢性阻塞性肺疾病氣道中的表達(dá)及意義[J]. 中華結(jié)核和呼吸雜志, 2004, 27(9): 585-588. |
| 6. | 陳智鴻, 祝蓉, 白莉, 等. 小發(fā)卡狀RNA介導(dǎo)的人呼吸道黏膜下腺細(xì)胞對(duì)水通道蛋白5的抑制及其對(duì)MUC-5AC表達(dá)的影響[J]. 中華結(jié)核和呼吸雜志, 2006, 29(2): 128-129. |
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| 8. | Vlaar AP, Wolthuis EK, Hofstra JJ, et al. Mechanical ventilation aggravates transfusion-related acute lung injury induced by MHC-I class antibodies[J]. Intensive Care Med, 2010, 36(5): 879-887. |
| 9. | Komatsu K, Jono H, Lim JH, et al. Glucocorticoids inhibit nontypeable Haemophilus influenzae-induced MUC-5AC mucin expression via MAPK phosphatase-1-dependent inhibition of p38 MAPK[J]. Biochem Biophys Res Commun, 2008, 377(3): 763-768. |
| 10. | Lim JH, Kim HJ, Komatsu K, et al. Differential regulation of Streptococcus pneumoniae induced human MUC-5AC mucin expression through distinct MAPK pathways[J]. Am J Transl Res, 2009, 1(3): 300-311. |
| 11. | Iyoda M, Shibata T, Kawaguchi M, et al. IL-17A and IL-17F stimulate chemokines via MAPK pathways (ERK1/2 and p38 but not JNK) in mouse cultured mesangial cells: synergy with TNF-alpha and IL-1beta[J]. Am J Physiol Renal Physiol, 2010, 298(3): F779-787. |
| 12. | Matsui T, Svensson CI, Hirata Y, et al. Release of prostaglandin E2 and nitric oxide from spinal microglia is dependent on activation of p38 mitogen-activated protein kinase[J]. Anesth Analg, 2010, 111(2): 554-60. |
| 13. | Jin JO, Song MG, Kim YN, et al. The mechanism of fucoidan-induced apoptosis in leukemic cells: involvement of ERK1/2, JNK, glutathione, and nitric oxide[J]. Mol Carcinog, 2010, 49(8): 771-782. |
- 1. Unoki T, Kawasaki Y, Mizutani T, et al. Effects of expiratory rib-cage compression on oxygenation, ventilation, and airway-secretion removal in patients receiving mechanical ventilation[J]. Respir Care, 2005, 50(11): 1430-1437.
- 2. O’Reilly M, Hooper SB, Allison BJ, et al. Persistent bronchiolar remodeling following brief ventilation of the very immature ovine lung[J]. Am J Physiol Lung Cell Mol Physiol, 2009, 297(5): 992-1001.
- 3. Durbin CG Jr, Perkins MP. Should tracheostomy be performed as early as 72 hours in patients requiring prolonged mechanical ventilation [J]. Respir Care, 2010, 55(1): 76-87.
- 4. EI Ferzli GT, Philips JB 3rd, Bulger A, et al. A pumpless lung assist device reduces mechanical ventilation-induced lung injury in juvenile piglets[J]. Pediatr Res, 2009, 66(6): 671-676.
- 5. 毛翎, 白春學(xué), 張敏, 等. 表皮生長(zhǎng)因子受體和黏蛋白在慢性阻塞性肺疾病氣道中的表達(dá)及意義[J]. 中華結(jié)核和呼吸雜志, 2004, 27(9): 585-588.
- 6. 陳智鴻, 祝蓉, 白莉, 等. 小發(fā)卡狀RNA介導(dǎo)的人呼吸道黏膜下腺細(xì)胞對(duì)水通道蛋白5的抑制及其對(duì)MUC-5AC表達(dá)的影響[J]. 中華結(jié)核和呼吸雜志, 2006, 29(2): 128-129.
- 7. Diaz JV, Brower R, Calfee CS, et al. Therapeutic strategies for severe acute lung injury[J]. Crit Care Med, 2010, 38(8): 1644-1650.
- 8. Vlaar AP, Wolthuis EK, Hofstra JJ, et al. Mechanical ventilation aggravates transfusion-related acute lung injury induced by MHC-I class antibodies[J]. Intensive Care Med, 2010, 36(5): 879-887.
- 9. Komatsu K, Jono H, Lim JH, et al. Glucocorticoids inhibit nontypeable Haemophilus influenzae-induced MUC-5AC mucin expression via MAPK phosphatase-1-dependent inhibition of p38 MAPK[J]. Biochem Biophys Res Commun, 2008, 377(3): 763-768.
- 10. Lim JH, Kim HJ, Komatsu K, et al. Differential regulation of Streptococcus pneumoniae induced human MUC-5AC mucin expression through distinct MAPK pathways[J]. Am J Transl Res, 2009, 1(3): 300-311.
- 11. Iyoda M, Shibata T, Kawaguchi M, et al. IL-17A and IL-17F stimulate chemokines via MAPK pathways (ERK1/2 and p38 but not JNK) in mouse cultured mesangial cells: synergy with TNF-alpha and IL-1beta[J]. Am J Physiol Renal Physiol, 2010, 298(3): F779-787.
- 12. Matsui T, Svensson CI, Hirata Y, et al. Release of prostaglandin E2 and nitric oxide from spinal microglia is dependent on activation of p38 mitogen-activated protein kinase[J]. Anesth Analg, 2010, 111(2): 554-60.
- 13. Jin JO, Song MG, Kim YN, et al. The mechanism of fucoidan-induced apoptosis in leukemic cells: involvement of ERK1/2, JNK, glutathione, and nitric oxide[J]. Mol Carcinog, 2010, 49(8): 771-782.