• 新疆醫(yī)科大學(xué)第五附屬醫(yī)院（烏魯木齊，830011）1 職防科，2 老年病科;

【摘要】　目的　 探討乳腺浸潤性導(dǎo)管癌中表皮鈣黏蛋白（E-cadherin，E-cad）的表達(dá)及其意義。　方法　 選取2005年1月－2009年12月的組織病理切塊，用免疫組織化學(xué)EnVision二步法檢測63例乳腺浸潤性導(dǎo)管癌（invasive ductal carcinoma，IDC）組織中E-cad的表達(dá)情況，設(shè)為IDC組；另檢測15例乳腺纖維腺瘤及15例乳腺小葉增生癥乳腺組織中E-cad的表達(dá)情況，設(shè)為對照組；比較兩組的E-cad表達(dá)。　結(jié)果　 E-cad在IDC組及對照組中表達(dá)陽性率分別為58.7%、80.0%；兩組間差異有統(tǒng)計(jì)學(xué)意義（P lt;0.05）。在乳腺IDC患者中，年齡 lt;38歲和≥38歲組的E-cad陽性表達(dá)率分別是54.2%、61.5%，兩組間差異無統(tǒng)計(jì)學(xué)意義（P gt;0.05）；腫塊直徑 lt;3 cm和≥3 cm組的E-cad陽性表達(dá)率分別是54.8%、66.7%，兩組間差異無統(tǒng)計(jì)學(xué)意義（P gt;0.05）；組織學(xué)分級為Ⅰ+Ⅱ級和Ⅲ級組的E-cad陽性表達(dá)率分別是76.3%、32.0%，兩組間差異有統(tǒng)計(jì)學(xué)意義（P lt;0.05）；無、有腋窩淋巴結(jié)轉(zhuǎn)移組的E-cad陽性表達(dá)率分別是78.3%、47.5%，兩組間差異有統(tǒng)計(jì)學(xué)意義（P lt;0.05）。　結(jié)論　 E-cad的表達(dá)與患者年齡及腫塊大小無關(guān)，而與組織學(xué)分級、淋巴結(jié)轉(zhuǎn)移相關(guān)。在乳腺浸潤性導(dǎo)管癌中，無淋巴結(jié)轉(zhuǎn)移者E-cad表達(dá)高于有淋巴結(jié)轉(zhuǎn)移者，提示E-cad是乳腺浸潤性導(dǎo)管癌發(fā)生淋巴結(jié)轉(zhuǎn)移的重要指標(biāo)。
【Abstract】　Objective　 To explore the expression of the protein E-cadherin (E-cad) in invasive ductal carcinoma (IDC) of the breast and its significance.　Methods　 We chose 63 cases of pathological wax with IDC between 2005 and 2009, and immunohistochemical EnVision method was used to detect the expression of E-cad protein in these cases which were designated to be the IDC group. At the same time, the E-cad expression in 15 cases of breast adenoma and another 15 cases of breast lobular hyperplasia were also detected, and these cases were designed to the the control group. The expression of E-cad in these two groups were compared.　Results　 The positive rates of E-cad protein expression in the IDC group and the control group were respectively 58.7% and 80.0% with a significant difference between the two groups (P lt;0.05). In the IDC group, the positive rates of E-cad protein expression in patients aged lt;38 and ≥38 years old were respectively 54.2% and 61.5% without a significant difference (P gt;0.05). The positive rates of E-cad protein expression for tumors with a diameter  lt;3 cm and ≥3 cm were respectively 54.8% and 66.7% without a significant difference (P gt;0.05). The positive rates of E-cad protein expression for class Ⅰ+Ⅱ tumors and class Ⅲ tumors were respectively 76.3% and 32.0% with a significant difference (P lt;0.05). The positive rates of E-cad protein expression for patients without and with axillary lymph node metastasis were respectively 78.3% and 47.5% with a significant difference (P lt;0.05).　Conclusions　 The expression of E-cad is correlated with histological classification and lymph node metastasis and was not related to tumor size and age of the patients. The expression of E-cad is higher in IDC patients without lymph node metastasis than that in IDC patients with lymph node metastasis, which indicates that E-cad is an important index for lymph node metastasis of IDC.

引用本文： 劉桂桃,李霞. 乳腺浸潤性導(dǎo)管癌組織中表皮鈣黏蛋白的表達(dá)及其意義. 華西醫(yī)學(xué), 2011, 26(7): 983-985. doi: 復(fù)制

1. 　陳杰, 李甘地. 病理學(xué)[M]. 北京: 人民衛(wèi)生出版社, 2005: 383.
2. 　谷化平, 尚培中, 劉艷茹. CD44v6和Ecadherin表達(dá)與乳腺癌生物學(xué)行為的關(guān)系[J]. 中國癌癥雜志, 2002, 12(3): 217-219.
3. 　Berx G, Staes K, van Hengel J, et al. Cloning and characterization of the human invasion suppressor gene E-cadherin (CDH1)[J]. Genomics, 1995, 26(2): 281-289.
4. 　Bussmahers MJG, Bokhoven AV, Mees SGM, et al. Molecular cloning and characterization of the human E cadherinc DNA[J]. Molbiol Rep, 1993, 17(2): 123-128.
5. 　Eidelman S, Damsky CH, Wheelock MJ, et al. Expression of the cell adhesion glycoprotein cell CAM120/80 in normal human tissues and tumors[J]. Am J Pathol, 1989, 135(1): 101-110.
6. 　Hermiston ML, Gordon JI. In vivo analysis of cadherin function in the mouse intestinal epithelium: essential roles in adhesion, maintenance of differentiation, and regulation of programmed cell death[J]. J Cell Biol 1995, 129(2): 489-506.
7. 　劉奕彬, 凌彬, 馬旭東, 等. E-cad和CD44v6在口腔鱗癌中的表達(dá)及意義[J]. 國際口腔醫(yī)學(xué)雜志, 2007, 34(5): 325-327.
8. 　易明福, 魏曉梅. E-cad、CD44v6基因表達(dá)與非小細(xì)胞肺癌淋巴轉(zhuǎn)移的關(guān)系[J]. 臨床肺科雜志, 2010, 15(2): 157-158.
9. 　Kurtz KA, Hoffman HT, Zimmerman MB, et al. Decreased E-cadherin but not beta-catenin expression is associated with vascular invasion and decreased survival in head and neck squamous carcinomas[J]. Otolaryngol Head Neck Surg, 2006, 134(1): 142-146.
10. 　Chen TT, Yuan DT, Wei B, et al. E-cadherin-mediated cell-cell contact is critical for induced pluripotent stem cell generation[J]. Stem Cells, 2010, 28(8): 1315-1325.
11. 　劉文清, 高雙全, 黃偉, 等. KAI1/CD與E-cadherin在結(jié)直腸癌的表達(dá)與預(yù)后的關(guān)系[J]. 華西醫(yī)學(xué), 2009, 24(8): 1954-1956.
12. 　Kwak JM, Min BW, Lee JH, et al. The prognostic significance of E-cadherin and liver intestine-cadherin expression in colorectal cancer[J]. Dis Colon Rectum, 2007, 50(11): 1873-1880.
13. 　Choi HN, Kim KR, Lee JH, et al. Serum response factor enhances liver metastasis of colorectal carcinoma via alteration of the E-cadherin/beta-catenin complex[J]. Oncol Rep, 2009, 21(1): 57-63.
14. 　李宇清, 梅開勇, 陸光明. 乳腺癌中E-cadherin、CerbB-2、p53、Ki-67的表達(dá)及其臨床意義[J]. 現(xiàn)代醫(yī)院, 2009, 9(8): 2-4.
  1. 1. 　陳杰, 李甘地. 病理學(xué)[M]. 北京: 人民衛(wèi)生出版社, 2005: 383.
  2. 2. 　谷化平, 尚培中, 劉艷茹. CD44v6和Ecadherin表達(dá)與乳腺癌生物學(xué)行為的關(guān)系[J]. 中國癌癥雜志, 2002, 12(3): 217-219.
  3. 3. 　Berx G, Staes K, van Hengel J, et al. Cloning and characterization of the human invasion suppressor gene E-cadherin (CDH1)[J]. Genomics, 1995, 26(2): 281-289.
  4. 4. 　Bussmahers MJG, Bokhoven AV, Mees SGM, et al. Molecular cloning and characterization of the human E cadherinc DNA[J]. Molbiol Rep, 1993, 17(2): 123-128.
  5. 5. 　Eidelman S, Damsky CH, Wheelock MJ, et al. Expression of the cell adhesion glycoprotein cell CAM120/80 in normal human tissues and tumors[J]. Am J Pathol, 1989, 135(1): 101-110.
  6. 6. 　Hermiston ML, Gordon JI. In vivo analysis of cadherin function in the mouse intestinal epithelium: essential roles in adhesion, maintenance of differentiation, and regulation of programmed cell death[J]. J Cell Biol 1995, 129(2): 489-506.
  7. 7. 　劉奕彬, 凌彬, 馬旭東, 等. E-cad和CD44v6在口腔鱗癌中的表達(dá)及意義[J]. 國際口腔醫(yī)學(xué)雜志, 2007, 34(5): 325-327.
  8. 8. 　易明福, 魏曉梅. E-cad、CD44v6基因表達(dá)與非小細(xì)胞肺癌淋巴轉(zhuǎn)移的關(guān)系[J]. 臨床肺科雜志, 2010, 15(2): 157-158.
  9. 9. 　Kurtz KA, Hoffman HT, Zimmerman MB, et al. Decreased E-cadherin but not beta-catenin expression is associated with vascular invasion and decreased survival in head and neck squamous carcinomas[J]. Otolaryngol Head Neck Surg, 2006, 134(1): 142-146.
  10. 10. 　Chen TT, Yuan DT, Wei B, et al. E-cadherin-mediated cell-cell contact is critical for induced pluripotent stem cell generation[J]. Stem Cells, 2010, 28(8): 1315-1325.
  11. 11. 　劉文清, 高雙全, 黃偉, 等. KAI1/CD與E-cadherin在結(jié)直腸癌的表達(dá)與預(yù)后的關(guān)系[J]. 華西醫(yī)學(xué), 2009, 24(8): 1954-1956.
  12. 12. 　Kwak JM, Min BW, Lee JH, et al. The prognostic significance of E-cadherin and liver intestine-cadherin expression in colorectal cancer[J]. Dis Colon Rectum, 2007, 50(11): 1873-1880.
  13. 13. 　Choi HN, Kim KR, Lee JH, et al. Serum response factor enhances liver metastasis of colorectal carcinoma via alteration of the E-cadherin/beta-catenin complex[J]. Oncol Rep, 2009, 21(1): 57-63.
  14. 14. 　李宇清, 梅開勇, 陸光明. 乳腺癌中E-cadherin、CerbB-2、p53、Ki-67的表達(dá)及其臨床意義[J]. 現(xiàn)代醫(yī)院, 2009, 9(8): 2-4.