• 貴陽醫(yī)學(xué)院（貴陽，550004）1 口腔修復(fù)教研室，2 附屬醫(yī)院口腔修復(fù)科;

【摘要】　目的　 探討微弧氧化（microarc oxidation，MAO）結(jié)合應(yīng)用于純鈦種植體表面處理的可行性。　方法　 根據(jù)對純鈦鈦片處理的不同將實驗分為對照組（A組，不作處理）、MAO組（B組，純鈦片上進(jìn)行MAO處理）及MAO加Ⅰ型膠原組（C組，純鈦片上MAO處理后吸附Ⅰ型膠原）。將成骨細(xì)胞培養(yǎng)于各組鈦片上，通過掃描電鏡、MTT法檢測不同時間點各組鈦片表面的細(xì)胞生長及增殖情況，并檢測堿性磷酸酶（alkaline phosphatase，ALP）活性。　結(jié)果　 掃描電鏡顯示成骨細(xì)胞在C組鈦片上細(xì)胞黏附情況優(yōu)于A、B組；MTT法及ALP活性檢測示培養(yǎng)3、6 d，成骨細(xì)胞在C組鈦片上的增殖及ALP活性與A、B組比較差異均有統(tǒng)計學(xué)意義（P lt;0.05）。　結(jié)論　 MAO結(jié)合Ⅰ型膠原處理的鈦片可更有效提高成骨細(xì)胞表面附著、增殖，且具有較高的ALP活性。
【Abstract】　Objective　 To study the feasibility of applying microarc oxidation (MAO) with collagen Ⅰ in surface modification of pure titanium.　Methods　 According to different processing methods, the pure titanium was divided into three groups: the control group (without surface modification, group A), MAO group (with microarc oxidation applied in pure titanium surface modification, group B), MAO+Ⅰ group (with microarc oxidation and collagen Ⅰ applied in pure titanium treatment, group C). Osteoblasts were cultured on the surface of titanium in each group, and the cell proliferation in each group was detected at different time points by scanning electron microscopy and MTT method. Moreover, the activity of alkaline phosphatase (ALP) was also detected.　Results　 Scanning electron microscopy showed that adhesion of osteoblasts for group C was better than group A and group B. MTT method and ALP activity detection indicated that there was a significant difference between group C and group A, B in cell proliferation and ALP activity on the third and sixth day of cultivation (P lt;0.05).　Conclusion　 MAO with collagen Ⅰ applied in surface modification of pure titanium may increase osteoblast attachment, and promote its proliferation and ALP activity.

引用本文： 冉丹丹,王永,張軍梅. 純鈦表面微弧氧化結(jié)合Ⅰ型膠原對成骨細(xì)胞黏附增殖的影響. 華西醫(yī)學(xué), 2011, 26(7): 1038-1042. doi: 復(fù)制

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14. 　何福明, 劉麗, 趙士芳, 等. 多孔純鈦種植體表面快速沉積鈣磷涂層的研究[J]. 生物醫(yī)學(xué)工程學(xué)雜志, 2007, 24(4): 806-811.
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17. 　Cassinelli C, Morra M, Bruzzone G, et al. Surface chemistry effects of topographic modification of titanium dental implant surfaces: 2. In vitro experiments[J]. Int J Oral Maxillofac Implants, 2003,18(1): 46-52.
18. 　Rössler S, Sewing A, Stölzel M, et al.Electrochemically assisted deposition of thin calcium phosphate coatings at near-physiological pH and temperature[J]. J Biomed Mater Res A, 2003, 64(4): 655-663.
  1. 1. 　宿玉成. 現(xiàn)代口腔種植學(xué)[M]. 北京: 人民衛(wèi)生出版社, 2004: 63-64.
  2. 2. 　Lind M. Growth factor stimulation of bone healing. Effects on osteoblasts, osteomies, and implants fixation[J]. Acta Orthop Scand Suppl, 1998, 283: 2-37.
  3. 3. 　嚴(yán)洪海, 胡玲華, 黃海蓉. 不同粗糙表面的純鈦種植體的耐腐蝕性評價[J]. 中國口腔種植學(xué)雜志, 2008, 13(4): 175-179.
  4. 4. 　Morra M. Biochemical modification of titanium surfaces: peptides and ECM proteins[J]. Eur Cell Mater, 2006,12: 1-15.
  5. 5. 　Morra M, Cassinelli C, Meda L, et al. Surface analysis and effects on interfacial bone microhardness of collagen-coated titanium implants: a rabbit model[J]. Int J Oral Maxillofac Implants, 2005, 20(1): 23-30.
  6. 6. 　司徒鎮(zhèn)強, 吳軍. 細(xì)胞培養(yǎng)[M]. 2版. 西安: 世界圖書出版公司, 2007: 161-162.
  7. 7. 　Jovanovic SA, Hunt DR, Bernard GW, et al. Bone reconstruction following implantation of rhBMP-2 and guided bone regeneration in canine alveolar ridge defects[J]. Clin Oral Implants Res, 2007,18(2): 224-230.
  8. 8. 　Schliephake H, Scharnweber D, Dard M, et al. Functionalization of dental implant surfaces using adhesion molecules [J]. J Biomed Mater Res B Appl Biomater, 2005, 73(1): 88-96.
  9. 9. 　Celic S, Katayama Y, Chilco PJ, et al. Type I collagen influence on gene expression in UMR106-06 osteoblast-like cells is inhibited by genistein[J]. J Endocrinol, 1998, 158(3): 377-388.
  10. 10. 　Shi S, Kirk M, Kahn AJ. The role of type I collagen in the regulation of the osteoblast phenotype[J]. J Bone Miner Res, 1996, 11(8): 1139-1145.
  11. 11. 　楊志明, 余希杰, 黃富國, 等. 外源性Ⅰ型膠原對人胚骨膜成骨細(xì)胞生物學(xué)特性性的影響 [J]. 華西醫(yī)科大學(xué)學(xué)報, 2001, 32(1): 1-4.
  12. 12. 　成煒, 陳吉華, 馬楚凡, 等. 純鈦種植體表面不同化學(xué)組成微弧氧化膜的結(jié)構(gòu)與成分分析[J]. 中國美容醫(yī)學(xué), 2007, 16(1): 107-110.
  13. 13. 　Zhu X, Kim KH, Jeong Y. Anodic oxide films containing Ca and P of titanium biomaterial[J]. Biomaterials, 2001, 22(16): 2199-2206.
  14. 14. 　何福明, 劉麗, 趙士芳, 等. 多孔純鈦種植體表面快速沉積鈣磷涂層的研究[J]. 生物醫(yī)學(xué)工程學(xué)雜志, 2007, 24(4): 806-811.
  15. 15. 　Tamai N, Myoui A, Tomita T, et al. Novel hydroxyapatite ceramics with an interconnective porous structure exhibit superior osteoconduction in vivo[J]. J Biomed Mater Res, 2002, 59(1): 110-117.
  16. 16. 　Sul YT. The significance of the surface properties of oxidized titanium to the bone response: special emphasis on potential biochemical bonding of oxidized titanium implant[J]. Biomaterials, 2003, 24(22): 3893-3907.
  17. 17. 　Cassinelli C, Morra M, Bruzzone G, et al. Surface chemistry effects of topographic modification of titanium dental implant surfaces: 2. In vitro experiments[J]. Int J Oral Maxillofac Implants, 2003,18(1): 46-52.
  18. 18. 　Rössler S, Sewing A, Stölzel M, et al.Electrochemically assisted deposition of thin calcium phosphate coatings at near-physiological pH and temperature[J]. J Biomed Mater Res A, 2003, 64(4): 655-663.