• 四川大學(xué)（成都，610041） 1 華西臨床醫(yī)學(xué)院，2 華西醫(yī)院消化內(nèi)科;

【摘要】　目的　 研究肥大細(xì)胞膜穩(wěn)定劑色甘酸二鈉（disordium cromoglycate，DC）對葡聚糖硫酸鈉（dextran sulfate sodium，DSS）誘導(dǎo)的大鼠急性結(jié)腸炎的影響。　方法　 出生80~100 d的雄性SD清潔級大鼠30只，體重180~250 g。30只大鼠隨機(jī)分為3組:正常對照組（A組）、潰瘍性結(jié)腸炎組（B組）和DC組（C組），每組各10只。B組和C組自由飲用40 g/L DSS溶液（4%） 7 d誘發(fā)急性結(jié)腸炎，同時C組每天按100 mg/kg腹腔注射DC 1次，A組和B組每天腹腔注射等量的生理鹽水。7 d后，處死各組大鼠。對各組大鼠行疾病活動指數(shù)評分，結(jié)腸組織行大體評分、組織學(xué)評分，檢測門靜脈血一氧化氮濃度，結(jié)腸組織髓過氧化物酶活性。　結(jié)果　 疾病活動指數(shù)評分、大體評分、組織學(xué)評分、一氧化氮濃度及髓過氧化物酶活性均表現(xiàn)為B組 gt;C組 gt;A組（P lt;0.05）。　結(jié)論　 肥大細(xì)胞膜穩(wěn)定劑DC對DSS誘導(dǎo)的大鼠急性結(jié)腸炎有一定的保護(hù)作用。
【Abstract】　Objective　 To observe the influence of the mast cell memebrane stabilizer, disordium cromoglycate (DC), on dextran sulfate sodium (DSS)-induced colitis in rats.　Methods　 Thirty male Sprague-Dawley (SD) rats aged 80 to 100 days with their weight ranged from 180 to 250 g were randomly divided into 3 groups: normal control group (group A), dextran sulfate sodium group (group B) and disordium cromoglycate group (group C), with 10 rats in each. Rats in group B and C drank 40 g/L DSS solution (4%) for 7 days to induce acute colitis. At the same time, intraperitoneal administration of DC (100 mg/kg) to rats in group C was carried out once a day, while the other two groups of rats were given the same amount of normal saline solution. Disease activity index (DAI), gross and histological evaluation were analyzed. NO concentration of blood from portal vein was measured. Myeloperoxidase (MPO) activity of colonic tissue was detected.　Results　 The experimental data of group C, including DAI, gross evaluation, histological assessment, NO concentration and MPO activity, were all significantly higher than those of group A (P lt;0.05), but lower than those of group B (P lt;0.05).　Conclusion　 Disordium cromoglycate can protect the colon of rats with DSS-induced acute colitis.

引用本文： 文光旭,馬洪升,程錢. 肥大細(xì)胞膜穩(wěn)定劑對大鼠急性結(jié)腸炎的影響. 華西醫(yī)學(xué), 2011, 26(8): 1142-1145. doi: 復(fù)制

1. 　歐陽欽. 潰瘍性結(jié)腸炎的研究進(jìn)展[J]. 現(xiàn)代消化及介入診療, 2008, 13(2): 100-103.
2. 　Magro F, Fraga S, Azevedo I, et al. Intestinal 5-hydroxytryptamine and mast cell infiltration in rat experimental colitis[J]. Dig Dis Sci, 2006, 51(3): 495-501.
3. 　Stoyanova Ⅱ, Gulubova MV. Mast cells and inflammatory mediators in chronic ulcerative colitis[J]. Acta Histochem, 2002, 104(2): 185-192.
4. 　Cooper HS, Murthy SN, Shah RS, et al. Clinicopathologic study of dextran sulfate sodium experimental murine colitis[J]. Lab Invest, 1993, 69(2): 238-249.
5. 　Okayasu I, Hatakeyama S, Yamada M, et al. A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice[J]. Gastroenterology, 1990, 98(3): 694-702.
6. 　Butzner JD, Parmar R, Bell CJ, et al. Butyrate enema therapy stimulates mucosal repair in experimental colitis in the rat[J]. Gut, 1996, 38(4): 568-573.
7. 　Dieleman L, Palmen MJ, Akol H, et al. Chronic experimental colitis induced by dextran sulphate sodium (DSS) is characterized by Th1 and Th2 cytokines[J]. Clin Exp Immunol, 1998, 114(3): 385-391.
8. 　Manktelow A, Meyer AA. Lack of correlation between decreases chemotaxis and susceptibility to infection in burned rats[J]. Trauma, 1986, 26(2): 143-148.
9. 　桑力軒, 劉漢立, 姜敏. 潰瘍性結(jié)腸炎發(fā)病機(jī)制研究進(jìn)展[J]. 世界華人消化雜志, 2007, 15(20): 2249-2254.
10. 　Wirtz S, Neurath MF. Gene transfer approaches for the treatment of inflammatory bowel disease[J]. Gene Ther, 2003, 10(10): 854-860.
11. 　Kashiwase Y, Inamura H, Morioka J, et al. Quantitative analysis of mast cells in benign and malignant colonic lesions: immunohistochemical study on formalin-fixed, paraffin-embedded tissues[J].Allergol immunopathol, 2008, 36(5): 271-276.
12. 　Raithel M, Winterkamp S, Pacurar A, et al. Release of mast cell tryptase from human colorectal mucosa in inflammatory bowel disease[J]. Scand J Gastroenterol, 2001, 36(2): 174-179.
13. 　Bischoff SC, Lorentz A, Schwengberg S, et al. Mast cells are an important cellular source of tumor necrosis factor alpha in human intestinal tissue[J]. Gut, 1999, 44(5): 643-652.
14. 　Kruschewski M, Buhr HJ. The vasculitis in IBD is associated with the degree of inflammation[J]. Dig Dis Sci, 2010, 55(3):733-738.
15. 　Seidelin JB, Nielsen OH. Epithelial apoptosis:cause or consequence of ulcerative colitis? [J]. Scand J Gastroenterol, 2009, 44(12): 1429-1434.
16. 　Braun A, Treede I, Gotthardt D, et al. Alterations of phospholipids concentration and species composition of the intestinal mucus barrier in ulcerative colitis:a clue to pathogenesis[J]. Inflamm Bowel Dis, 2009, 15(11): 1705-1720.
17. 　Kawada M, Arihiro A, Mizoguchi E. Insights from advances in research of chemically induced experimental models of human inflammatory bowel disease[J]. World J Gastroenterol, 2007, 13(42): 5581-5593.
18. 　Chung HL, YUE GG, To KF, et al. Effect of scutellariae radix extract on experimental dextran-sulfate sodium-induced colitis in rats[J].World J Gastroenterol, 2007, 13(42): 5605-5611.
19. 　Damiani CR, Benetton CA, Stoffel C, et al. Oxidative stress and metabolism in animal model of colitis induced by dextran sulfate sodium[J].J Gastroenterol Hepatol, 2007, 22(11): 1846-1851.
20. 　Arafa HM, Hemeida RA, El-bahrawy AI, et al. Prophylactic role of curcumin in dextran sulfate sodium(DSS)-induced ulcerative colitis murine model[J]. Food Che Toxicol, 2009, 47(6): 1131-1137.
21. 　Boughton-smith NK, Evans SM, Whittle BR, et al. Nitric oxide synthase activity in ulcerative colitis and Crohn’s disease[J]. Lancet, 1993, 342(8867): 338-340.
22. 　Rachmilewitz D, Stamler JS, Karmeli F, et al. Peroxynitrite-induced rat colitis--a new model of colonic inflammation[J]. Gastroenterology, 1993, 105(6): 1681-1688.
23. 　Rumi G, Tsubouchi R, Nishio H, et al. Dual role of endogenous nitric oxide in development of dextran sodium sulfate-induced colitis in rats[J]. J Physiol Pharmacol, 2004, 55(4): 823-836.
24. 　周國勝, 吳正祥. 一氧化氮與炎癥性腸病[J]. 安徽醫(yī)藥, 2008, 12(11): 1010-1012.
25. 　Villegas I, Alarcón DC, Orjales A, et al. A new flavonoid derivative, dosmalfate, attenuates the development of dextran sulfate sodium-induced colitis in mice[J]. Int Immunopharmacol, 2003, 3(13-14): 1731-1741.
  1. 1. 　歐陽欽. 潰瘍性結(jié)腸炎的研究進(jìn)展[J]. 現(xiàn)代消化及介入診療, 2008, 13(2): 100-103.
  2. 2. 　Magro F, Fraga S, Azevedo I, et al. Intestinal 5-hydroxytryptamine and mast cell infiltration in rat experimental colitis[J]. Dig Dis Sci, 2006, 51(3): 495-501.
  3. 3. 　Stoyanova Ⅱ, Gulubova MV. Mast cells and inflammatory mediators in chronic ulcerative colitis[J]. Acta Histochem, 2002, 104(2): 185-192.
  4. 4. 　Cooper HS, Murthy SN, Shah RS, et al. Clinicopathologic study of dextran sulfate sodium experimental murine colitis[J]. Lab Invest, 1993, 69(2): 238-249.
  5. 5. 　Okayasu I, Hatakeyama S, Yamada M, et al. A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice[J]. Gastroenterology, 1990, 98(3): 694-702.
  6. 6. 　Butzner JD, Parmar R, Bell CJ, et al. Butyrate enema therapy stimulates mucosal repair in experimental colitis in the rat[J]. Gut, 1996, 38(4): 568-573.
  7. 7. 　Dieleman L, Palmen MJ, Akol H, et al. Chronic experimental colitis induced by dextran sulphate sodium (DSS) is characterized by Th1 and Th2 cytokines[J]. Clin Exp Immunol, 1998, 114(3): 385-391.
  8. 8. 　Manktelow A, Meyer AA. Lack of correlation between decreases chemotaxis and susceptibility to infection in burned rats[J]. Trauma, 1986, 26(2): 143-148.
  9. 9. 　桑力軒, 劉漢立, 姜敏. 潰瘍性結(jié)腸炎發(fā)病機(jī)制研究進(jìn)展[J]. 世界華人消化雜志, 2007, 15(20): 2249-2254.
  10. 10. 　Wirtz S, Neurath MF. Gene transfer approaches for the treatment of inflammatory bowel disease[J]. Gene Ther, 2003, 10(10): 854-860.
  11. 11. 　Kashiwase Y, Inamura H, Morioka J, et al. Quantitative analysis of mast cells in benign and malignant colonic lesions: immunohistochemical study on formalin-fixed, paraffin-embedded tissues[J].Allergol immunopathol, 2008, 36(5): 271-276.
  12. 12. 　Raithel M, Winterkamp S, Pacurar A, et al. Release of mast cell tryptase from human colorectal mucosa in inflammatory bowel disease[J]. Scand J Gastroenterol, 2001, 36(2): 174-179.
  13. 13. 　Bischoff SC, Lorentz A, Schwengberg S, et al. Mast cells are an important cellular source of tumor necrosis factor alpha in human intestinal tissue[J]. Gut, 1999, 44(5): 643-652.
  14. 14. 　Kruschewski M, Buhr HJ. The vasculitis in IBD is associated with the degree of inflammation[J]. Dig Dis Sci, 2010, 55(3):733-738.
  15. 15. 　Seidelin JB, Nielsen OH. Epithelial apoptosis:cause or consequence of ulcerative colitis? [J]. Scand J Gastroenterol, 2009, 44(12): 1429-1434.
  16. 16. 　Braun A, Treede I, Gotthardt D, et al. Alterations of phospholipids concentration and species composition of the intestinal mucus barrier in ulcerative colitis:a clue to pathogenesis[J]. Inflamm Bowel Dis, 2009, 15(11): 1705-1720.
  17. 17. 　Kawada M, Arihiro A, Mizoguchi E. Insights from advances in research of chemically induced experimental models of human inflammatory bowel disease[J]. World J Gastroenterol, 2007, 13(42): 5581-5593.
  18. 18. 　Chung HL, YUE GG, To KF, et al. Effect of scutellariae radix extract on experimental dextran-sulfate sodium-induced colitis in rats[J].World J Gastroenterol, 2007, 13(42): 5605-5611.
  19. 19. 　Damiani CR, Benetton CA, Stoffel C, et al. Oxidative stress and metabolism in animal model of colitis induced by dextran sulfate sodium[J].J Gastroenterol Hepatol, 2007, 22(11): 1846-1851.
  20. 20. 　Arafa HM, Hemeida RA, El-bahrawy AI, et al. Prophylactic role of curcumin in dextran sulfate sodium(DSS)-induced ulcerative colitis murine model[J]. Food Che Toxicol, 2009, 47(6): 1131-1137.
  21. 21. 　Boughton-smith NK, Evans SM, Whittle BR, et al. Nitric oxide synthase activity in ulcerative colitis and Crohn’s disease[J]. Lancet, 1993, 342(8867): 338-340.
  22. 22. 　Rachmilewitz D, Stamler JS, Karmeli F, et al. Peroxynitrite-induced rat colitis--a new model of colonic inflammation[J]. Gastroenterology, 1993, 105(6): 1681-1688.
  23. 23. 　Rumi G, Tsubouchi R, Nishio H, et al. Dual role of endogenous nitric oxide in development of dextran sodium sulfate-induced colitis in rats[J]. J Physiol Pharmacol, 2004, 55(4): 823-836.
  24. 24. 　周國勝, 吳正祥. 一氧化氮與炎癥性腸病[J]. 安徽醫(yī)藥, 2008, 12(11): 1010-1012.
  25. 25. 　Villegas I, Alarcón DC, Orjales A, et al. A new flavonoid derivative, dosmalfate, attenuates the development of dextran sulfate sodium-induced colitis in mice[J]. Int Immunopharmacol, 2003, 3(13-14): 1731-1741.