【摘要】 目的 研究ΔNP63/TAP63在上皮性卵巢腫瘤組織中的表達及其與臨床病理特征的關系。 方法 運用熒光定量聚合酶鏈反應方法檢測2002年-2004年54例卵巢上皮性腫瘤中ΔNP63/TAP63的基因水平。 結(jié)果 33例卵巢上皮細胞癌組織中ΔNP63的表達高于21例良性上皮性腫瘤中組織。卵巢上皮細胞癌中的表達強度與腫瘤組織病理學分期相關（P lt;0.05），良性腫瘤的表達低于惡性腫瘤（P lt;0.05）。ΔNP63的表達高于TAP63（P lt;0.05）；各組間TAP63的表達差異無統(tǒng)計學意義（P gt;0.05）。 結(jié)論 ΔNP63在上皮性卵巢癌中高表達，可能成為上皮性卵巢癌診斷及預后的分子標志物。
【Abstract】 Objective To explore the expression of ΔNP63 / TAP63 in human epithelial ovarian tumor tissues and its relationship with the clinicopathological features. Methods Fluorescent quantitative PCR method was used to detect 54 cases of ΔNP63 / TAP63 gene level in 54 patients with epithelial ovarian tumors diagnosed between 2002 and 2004. Results ΔNP63 expression in the 33 cases of ovarian epithelial cell carcinoma was higher than that in the 21 cases of benign epithelial tumor tissue. The expression in ovarian epithelial cell carcinoma was concerned with the pathological staging of tumor (P lt;0.05); the expression in benign tumors was lower than that in the malignant tumors (P lt;0.05). In all cases, the expression of ΔNP63 was higher than that of TAP63 (P lt;0.05); the difference in the expression of TAP63 among the groups was not significant (P gt;0.05). Conclusion ΔNP63 in epithelial ovarian cancer is highly expressed, which may become the molecular makers with diagnosis and prognosis of epithelial ovarian cancer diagnosis in the future.
引用本文： 王滟,楊業(yè)洲,謝蘭,楊年,郭波. ΔNP63/TAP63在卵巢上皮性腫瘤組織中的表達及其與臨床病理關系. 華西醫(yī)學, 2011, 26(9): 1379-1381. doi: 復制
| 1. | 曹澤毅. 中華婦產(chǎn)科學[M]. 北京: 人民衛(wèi)生出版社, 2004: 2163. |
| 2. | Kocken M, Baalbergen A, Snijders PJ, et al. High-risk human papillomavirus seems not involved in DES-related and of limited importance in nonDES related clear-cell carcinoma of the cervix. [J]. Gynecol Oncol, 2011, 122(2): 297-302. |
| 3. | 王曉惠, 彭芝蘭, 雷軍強, 等. 卡鉑誘導卵巢癌細胞株OVCA_3凋亡中p63及p53基因mRNA的變化[J]. 蘭州大學學報(醫(yī)學版), 2010, 36(4): 8-10. |
| 4. | Osada M, Ohba M, Kawabara C, et al. Cloning and functional analysis of human P51, which structurally and functional resembles P53[J]. Nat Med, 1998, 7(4): 839-843. |
| 5. | Levrero M, Laurenzi VD, Costanzo A, et al. Structure,function and regulation of P63 and P73[J]. Cell Death Differ [J], 1999, 6(12): 1154-1161. |
| 6. | Irwin MS, Kaelin WG. Role of the newer P53 family proteins in malignancy[J]. Apoptosis, 2001, 6(10): 17-19. |
| 7. | Parsa R, Yang A, McKeon F, et al. Association of P63 with proliferative potential in n ormal and neoplastic human keratinocytes[J]. J Invest Dermatol, 1999, 113(10): 1099-1105. |
| 8. | Glickman JN, Yang A, Shahsafaei A, et al. Expression of P53-related protein P63 in the gastrointestinal tract and in esophageal metaplastic disorders [J]. Hum Pathol, 2001, 32(11): 1157-1165. |
| 9. | Wu GJ, Nomoto SJ, Hoque MO, et al. ΔNP63α and TAP63α regulate tanscription of genes with distinct biological function in cancer and development [J]. Cancer Res, 2003, 63(15): 2351-2357. |
| 10. | 司少艷, 張建中. P63基因研究進展[J]. 世界華人消化雜志, 2003, 11(5): 606-609. |
| 11. | Elsa R, Flores, Kenneth Y, et al. P63 and P73 are required for P53 dependent apoptosis in response to DNA damage[J]. Nature, 2002, 416(4): 560-564. |
| 12. | Wu GJ, Nomoto SJ, Hoque MO, et al. ΔNP63αand TAP63αregulate tanscription of genes with distinct biological function in cancer and development [J]. Cancer Res, 2003, 63(15): 2351-2357. |
- 1. 曹澤毅. 中華婦產(chǎn)科學[M]. 北京: 人民衛(wèi)生出版社, 2004: 2163.
- 2. Kocken M, Baalbergen A, Snijders PJ, et al. High-risk human papillomavirus seems not involved in DES-related and of limited importance in nonDES related clear-cell carcinoma of the cervix. [J]. Gynecol Oncol, 2011, 122(2): 297-302.
- 3. 王曉惠, 彭芝蘭, 雷軍強, 等. 卡鉑誘導卵巢癌細胞株OVCA_3凋亡中p63及p53基因mRNA的變化[J]. 蘭州大學學報(醫(yī)學版), 2010, 36(4): 8-10.
- 4. Osada M, Ohba M, Kawabara C, et al. Cloning and functional analysis of human P51, which structurally and functional resembles P53[J]. Nat Med, 1998, 7(4): 839-843.
- 5. Levrero M, Laurenzi VD, Costanzo A, et al. Structure,function and regulation of P63 and P73[J]. Cell Death Differ [J], 1999, 6(12): 1154-1161.
- 6. Irwin MS, Kaelin WG. Role of the newer P53 family proteins in malignancy[J]. Apoptosis, 2001, 6(10): 17-19.
- 7. Parsa R, Yang A, McKeon F, et al. Association of P63 with proliferative potential in n ormal and neoplastic human keratinocytes[J]. J Invest Dermatol, 1999, 113(10): 1099-1105.
- 8. Glickman JN, Yang A, Shahsafaei A, et al. Expression of P53-related protein P63 in the gastrointestinal tract and in esophageal metaplastic disorders [J]. Hum Pathol, 2001, 32(11): 1157-1165.
- 9. Wu GJ, Nomoto SJ, Hoque MO, et al. ΔNP63α and TAP63α regulate tanscription of genes with distinct biological function in cancer and development [J]. Cancer Res, 2003, 63(15): 2351-2357.
- 10. 司少艷, 張建中. P63基因研究進展[J]. 世界華人消化雜志, 2003, 11(5): 606-609.
- 11. Elsa R, Flores, Kenneth Y, et al. P63 and P73 are required for P53 dependent apoptosis in response to DNA damage[J]. Nature, 2002, 416(4): 560-564.
- 12. Wu GJ, Nomoto SJ, Hoque MO, et al. ΔNP63αand TAP63αregulate tanscription of genes with distinct biological function in cancer and development [J]. Cancer Res, 2003, 63(15): 2351-2357.