• 成都中醫(yī)藥大學(xué)第二臨床醫(yī)學(xué)院 第二附屬醫(yī)院生殖中心（成都，610041）;

【摘要】 目的  探討不同年齡段和不同內(nèi)膜準(zhǔn)備方法對凍融胚胎移植（FET）妊娠率的影響。 方法  回顧性分析2007年3月-2009年8月107例患者131個(gè)FET周期，比較不同年齡段、不同內(nèi)膜準(zhǔn)備方法的妊娠情況。 結(jié)果  ① lt;29歲、30～34歲、≥35歲患者的周期臨床妊娠率分別為45.28%、37.88%、33.33%，比較無統(tǒng)計(jì)學(xué)意義（P gt;0.05）。②自然周期和激素替代周期內(nèi)膜準(zhǔn)備，兩種方法患者平均年齡、平均移植胚胎數(shù)、胚胎評分及胚胎細(xì)胞數(shù)比較無統(tǒng)計(jì)學(xué)意義（P gt;0.05），但自然周期的胚胎種植率及周期臨床妊娠率均明顯低于激素替代周期（16.78%比29.21%；29.51%比50.00%），比較有統(tǒng)計(jì)學(xué)意義（P lt;0.05）。 結(jié)論  患者年齡不影響FET周期臨床妊娠率，激素替代周期內(nèi)膜準(zhǔn)備的FET胚胎種植率及周期臨床妊娠率明顯高于自然周期。
【Abstract】 Objective  To analyze the effect of age and method of preparing endometrium on the pregnancy rate of frozen-thawed embryo transfer (FET) among infertile women. Methods  Retrospective analysis was made on 131 FET cycles in 107 patients from March 2007 to August 2009. The relationship between pregnancy rate of FET and following factors were analyzed: age, the method of preparing uterus endometrium. Results  ①There was no significant difference in pregnancy rate of FET among ＜29, 30 - 34, and ≥35 years old patients (45.28%, 37.88%, and 33.33%, respectively) (P gt;0.05). ②There was no significant difference in patients’ age, the average embryo amount, embryo score, the cell amount in every embryo between the FET with hormone replacement therapy (HRT) and the FET with natural cycle (NC). The embryo implantation rate and pregnancy rate of FET with NC were lower significantly than those of FET with HRT (16.78% vs 29.21%; 29.51% vs 50.00%) (P＜0.05). Conclusion  Age might not influence pregnancy rate of FET. FET with HRT has higher implantation rate and pregnancy rate than FET with NC.

引用本文： 賀貞,朱明輝,楊丹. 年齡和內(nèi)膜準(zhǔn)備法對凍融胚胎移植妊娠率的影響. 華西醫(yī)學(xué), 2010, 25(7): 1310-1312. doi: 復(fù)制

版權(quán)信息： ?四川大學(xué)華西醫(yī)院華西期刊社《華西醫(yī)學(xué)》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

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2. Ubaldi CF, Rienzi L, Baroni E, et al. Cumulative pregnancy rates after transfer of fresh and thawed embryos[J]. Eur J Obstetrics Gynecol Reprod Biol, 2004, 115(7): 106-109.
3. Alama P, Melo MAB, Garcia G, et al. Higher ongoing pregnancy rates in blastocyst transfer of frozen-thawed embryos in natural cycles than in hormone replacement therapy cycles[J]. Fertil Steril, 2007, 88(9): s161.
4. 李豫峰, 朱桂金, 章漢旺, 等. 三種凍融胚胎移植內(nèi)膜準(zhǔn)備方法的比較[J]. 生殖與避孕, 2009, 29(2): 113-116.
5. 譚真. 影響凍融胚胎移植結(jié)果的因素分析[J]. 中華現(xiàn)代婦產(chǎn)科學(xué)雜志, 2005, 2(8): 692-695.
6. Borini A, Maccolini A, Bianchi L, et al. Oocyte donation program: pregnancy and implantation rates in women of different ages sharing oocytes from single donor[J]. Fertil Steril, 1996, 65(1): 94-97.
7. 李潔, 莊廣倫. 子宮內(nèi)膜功能異常與反復(fù)流產(chǎn)[J]. 中國實(shí)用婦科與產(chǎn)科雜志, 1999, 15(3): 141-143.
8. Muasher SJ, Kruithoff C, Simonetti S, et al. Controlled preparation of the endometrium with exogenous steroids for the transfer of frozen-thawed pre-embryos in patients with anovulatory or irregular cycles[J]. Hum Reprod, 1991, 6(3): 443-445.
9. Dal Prato L, Borini A, Cattoli M, et al. Endometrial preparation for frozen-thawed embryo transfer with or without pretreatment with gonadotropin-releasing hormone agonist[J]. Fertil Steril, 2002, 77(5): 956-960.
  1. 1. Trounson A, Mohr L. Human pregnancy following cryopreservation, thawing and transfer of an eight-cell stage humanembryo[J]. Nature, 1983, 305(5936): 707-709.
  2. 2. Ubaldi CF, Rienzi L, Baroni E, et al. Cumulative pregnancy rates after transfer of fresh and thawed embryos[J]. Eur J Obstetrics Gynecol Reprod Biol, 2004, 115(7): 106-109.
  3. 3. Alama P, Melo MAB, Garcia G, et al. Higher ongoing pregnancy rates in blastocyst transfer of frozen-thawed embryos in natural cycles than in hormone replacement therapy cycles[J]. Fertil Steril, 2007, 88(9): s161.
  4. 4. 李豫峰, 朱桂金, 章漢旺, 等. 三種凍融胚胎移植內(nèi)膜準(zhǔn)備方法的比較[J]. 生殖與避孕, 2009, 29(2): 113-116.
  5. 5. 譚真. 影響凍融胚胎移植結(jié)果的因素分析[J]. 中華現(xiàn)代婦產(chǎn)科學(xué)雜志, 2005, 2(8): 692-695.
  6. 6. Borini A, Maccolini A, Bianchi L, et al. Oocyte donation program: pregnancy and implantation rates in women of different ages sharing oocytes from single donor[J]. Fertil Steril, 1996, 65(1): 94-97.
  7. 7. 李潔, 莊廣倫. 子宮內(nèi)膜功能異常與反復(fù)流產(chǎn)[J]. 中國實(shí)用婦科與產(chǎn)科雜志, 1999, 15(3): 141-143.
  8. 8. Muasher SJ, Kruithoff C, Simonetti S, et al. Controlled preparation of the endometrium with exogenous steroids for the transfer of frozen-thawed pre-embryos in patients with anovulatory or irregular cycles[J]. Hum Reprod, 1991, 6(3): 443-445.
  9. 9. Dal Prato L, Borini A, Cattoli M, et al. Endometrial preparation for frozen-thawed embryo transfer with or without pretreatment with gonadotropin-releasing hormone agonist[J]. Fertil Steril, 2002, 77(5): 956-960.