• 汕頭大學(xué)醫(yī)學(xué)院第二附屬醫(yī)院外三科（廣東汕頭，515041）;

【摘要】 目的  觀察已構(gòu)建的含胸苷激酶（TK）自殺基因的重組腺病毒（ADV-TK）對(duì)肝癌細(xì)胞的體外殺傷作用和對(duì)肝癌裸鼠移植瘤的治療效果。 方法  將ADV-TK體外感染人肝癌細(xì)胞株SMMC-7721，噻唑藍(lán)（MTT）法檢測(cè)受感染的SMMC-7721細(xì)胞被不同濃度更昔洛韋（GCV）作用后的細(xì)胞存活率情況。構(gòu)建肝癌SMMC-7721裸鼠移植瘤模型，觀察腫瘤注射重組腺病毒ADV-TK結(jié)合GCV治療移植瘤的變化。 結(jié)果  相同滴度的重組腺病毒與不同濃度的GCV作用于肝癌細(xì)胞株SMMC-7721后，MTT法檢測(cè)到細(xì)胞的存活率隨著GCV濃度的增加而不斷降低。動(dòng)物實(shí)驗(yàn)中ADV-TK治療組腫瘤體積明顯小于對(duì)照組（ADV-null及NS）（P lt;0.01）。 結(jié)論  重組腺病毒ADV-TK對(duì)肝癌SMMC-7721細(xì)胞的體外增殖和裸鼠體內(nèi)的移植瘤生長(zhǎng)均有明顯的抑制作用。
【Abstract】 Objective  To explore the inhibitory effect of recombinant adenovirus containing TK gene (ADV-TK) on transfected human liver cancer cells SMMC-7721 in vitro and murine transplanted hepatocarcinoma in vivo. Methods  SMMC-7721 cells transfected with ADV-TK were exposed to medium with GCV. The cell viability was measured by MTT assays. In the established model of SMMC-7721 human liver cancer, nude mice underwent intratumoral injection with 1 109 pfu ADV-TK, the control vector (ADV-null) or normal saline (NS) and again 7 days later, twice for all. GCV was given at a dose of l00 mg/(kg?d) on the following day of injection for 10 days. The tumor inhibitory effect was observed by measuring the tumor sizes. Results  After transfected by ADV-TK in vitro, and combined with GCV, the cell growth of SMMC-7721 cell were significantly suppressed. The result of in vivo assay showed that tumor volumes in treatment group were apparently smaller than that in the control group (P lt;0.01). Conclusion  Recombinant adenovirus combined with GCV shows a significant inhibitory effect on SMMC-7721 cells in vitro and murine transplanted hepatocarcinoma in vivo.

引用本文： 陳耿臻,韓慧,許銘炎,鄧小玲. 重組腺病毒ADV-TK對(duì)肝癌抑制作用的實(shí)驗(yàn)研究. 華西醫(yī)學(xué), 2010, 25(11): 1941-1943. doi: 復(fù)制

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  3. 3. 　Portsmouth D, Hlavaty J, Renner M. Suicide genes for cancer therapy [J]. Mol Aspects Med, 2007, 28 (1): 4-41. .
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  5. 5. 　Stefani AL, Barzon L, Castagliuolo I, et al. Systemic efficacy of combined suicide/cytokine gene therapy in a murinemodel ofhepatocellular carcinoma[J]. J Hepatol, 2005, 42(5): 728-735. .
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