• 1 莆田市第一醫(yī)院病理科（福建莆田，351100）; 2 莆田學(xué)院醫(yī)學(xué)院病理教研室;

【摘要】 目的  觀察CyclinD1在食管鱗狀細(xì)胞癌和癌周正常食管組織的表達(dá)，從量化角度闡明其在食管鱗狀細(xì)胞癌組織中表達(dá)的病理學(xué)意義及其與CyclinE表達(dá)的關(guān)系。 方法  用免疫組化MaxVision法檢測(cè)CyclinD1、CyclinE在食管鱗狀細(xì)胞癌和癌周正常食管組織的表達(dá)，Image-pro plus圖像分析軟件對(duì)其表達(dá)強(qiáng)度進(jìn)行定量分析，并用表達(dá)的陽(yáng)性單位（positive unit，PU）反映其表達(dá)強(qiáng)度。 結(jié)果  CyclinD1蛋白在食管鱗狀細(xì)胞癌癌細(xì)胞核中表達(dá)的PU值高于正常食管組織（P lt;0.001）；CyclinD1蛋白表達(dá)的PU值與食管鱗狀細(xì)胞癌分化程度有關(guān)（P lt;0.001）；浸潤(rùn)漿膜層組的CyclinD1蛋白表達(dá)的PU值高于無(wú)漿膜層浸潤(rùn)組（P=0.037）；伴淋巴結(jié)轉(zhuǎn)移組的CyclinD1蛋白表達(dá)的PU值高于無(wú)淋巴結(jié)轉(zhuǎn)移組（P=0.012）；CyclinD1與CyclinE蛋白在食管鱗狀細(xì)胞癌中表達(dá)呈正相關(guān)（r=0.650，P lt;0.01）。 結(jié)論  CyclinD1在食管鱗狀細(xì)胞癌中表達(dá)上調(diào)，且與生物學(xué)特性相關(guān)，可作為判斷腫瘤患者預(yù)后不良的標(biāo)志之一；CyclinD1和CyclinE可能在食管癌癌變過程中起協(xié)同作用。
/【Abstract】　Objective　 To observe the expression of CyclinD1 and its correlation with the expression of CyclinE in esophagus squamous carcinoma.　Methods　 The expressions of CyclinD1 and CyclinE protein were detected by immunohistochemistry (MaxVision method) using the monoclonal antibody and their intensities were assessed quantitatively by Image-pro plus image analysis system.　Results　 The monoclonal antibody was detected in specific cell nucleus. The positive unit (PU) value of CyclinD1 was larger in the esophagus squamous carcinoma than that in the normal esophagus tissue with a statistically significant difference (P lt;0.001). The expression of CyclinD1 was correlated with differentiation of esophagus squamous carcinoma (P lt;0.001) and infiltration degree of esophagus squamous carcinoma (P=0.037). The PU value of CyclinD1 of the nuclei was larger in esophagus squamous carcinoma with lymph node metastasis than those without lymph node metastasis (P=0.012). There was a positive correlation between CyclinD1 protein and CyclinE protein (r=0.650, P lt;0.01).　Conclusion　 The positive expression of CyclinD1 is up-regulated in the esophagus squamous carcinoma. CyclinD1 protein relates to the clinicopathological characteristics and prognostic value in esophagus squamous carcinoma. CyclinD1 expression is bly associated with CyclinE expression in esophagus squamous carcinoma.

引用本文： 黃漢興,張金添. 食管鱗狀細(xì)胞癌CyclinD1的表達(dá)及其與CyclinE的相關(guān)性研究. 華西醫(yī)學(xué), 2010, 25(11): 1944-1946. doi: 復(fù)制

1. 　申洪, 陸藥丹. 免疫組織化學(xué)染色的定量方法研究[J]. 生物醫(yī)學(xué)工程學(xué)雜志, 1993, 10(3): 281-284.
2. 　申洪. 免疫組織顯色反應(yīng)強(qiáng)度定量方法研究(Ⅱ)[J]. 細(xì)胞與分子免疫學(xué)雜志, 1994, 10(4): 33-35.
3. 　申洪. 免疫組織化學(xué)染色的定量方法研究(Ⅲ)[J]. 中國(guó)組織化學(xué)與細(xì)胞化學(xué)雜志, 1995, 4(1): 89-92.
4. 　Donnellan R, Chetty R. CyclinD1 and human neoplasia[J]. Mol Pathol, 1998, 51(1): l-7.
5. 　Liu B, Ren Z, Shi Y, et al. Activation of signal transducers and activators of transcription 3 and overexpression of its target gene CyclinD1 in laryngeal carcinomas[J]. Laryngoscope, 2008, 118(11): 1976-1980.
6. 　Tsai-Turton M, Luong BT, Tan Y, et al. Cyclophosphamide-induced apoptosis in COV434 human granulose cells involves oxidative stress and glutathione depletion[J]. Toxicol Sci, 2007, 98(1): 216-230.
7. 　Bilalovic N, Vranic S, Basic H, et al. Immunohistochemical evaluation of CyclinD1 in breast cancer[J]. Croat Med J, 2005, 46(3): 382-384.
8. 　Zhu SC, Li R, Wang YX, et al. Impact of simultaneous assay, the PCNA, cyclinD1, and DNA content with specimens before and after preoperative radiotherapy on prognosis of esophageal cancer possible incorporation into clinical TNM staging system[J]. World J Gastroenterol, 2005, 11(25): 3823-3829.
9. 　胡玉林, 劉志輝, 趙于天, 等. P53和CyclinD1蛋白的表達(dá)對(duì)食管癌預(yù)后意義的研究[J]. 哈爾濱醫(yī)科大學(xué)學(xué)報(bào), 2007, 41(1): 52-54.
10. 　金春亭, 張林西, 李海軍, 等. 人食管鱗癌中細(xì)胞周期調(diào)節(jié)基因蛋白的表達(dá)[J]. 世界華人消化雜志, 2005, 13(15): 1909-1912.
11. 　Fu ZJ, Ma ZY, Wang QR, et al. Overexpression of CyclinD1 and underexpression of p16 correlate with lymph node metastases in laryngeal squamous cell carcinoma in Chinese patients[J]. Clin Exp Metastasis, 2008, 25(8): 887-892.
12. 　Bahnassy AA, Zekri AR, Houssinis EL, et al. CyclinA and cyclinD, as significant prognostic markers in colorectal patients[J]. BMC Gastroenteral, 2004, 4(1): 22.
13. 　田冬艷, 王德華, 劉永紅, 等. Survivin和CyclinD1在卵巢癌中的表達(dá)和相關(guān)性[J]. 天津醫(yī)科大學(xué)學(xué)報(bào), 2009, 15(1): 81-87.
14. 　Shao J, Teraishi F, Katsuda K, et al. p53 inhibits adriamycin-induced down-regulation of cyclin D1 expression in human cancer cells[J]. Biochem Biophys Res Commun, 2002, 290(3): 1101-1107.
  1. 1. 　申洪, 陸藥丹. 免疫組織化學(xué)染色的定量方法研究[J]. 生物醫(yī)學(xué)工程學(xué)雜志, 1993, 10(3): 281-284.
  2. 2. 　申洪. 免疫組織顯色反應(yīng)強(qiáng)度定量方法研究(Ⅱ)[J]. 細(xì)胞與分子免疫學(xué)雜志, 1994, 10(4): 33-35.
  3. 3. 　申洪. 免疫組織化學(xué)染色的定量方法研究(Ⅲ)[J]. 中國(guó)組織化學(xué)與細(xì)胞化學(xué)雜志, 1995, 4(1): 89-92.
  4. 4. 　Donnellan R, Chetty R. CyclinD1 and human neoplasia[J]. Mol Pathol, 1998, 51(1): l-7.
  5. 5. 　Liu B, Ren Z, Shi Y, et al. Activation of signal transducers and activators of transcription 3 and overexpression of its target gene CyclinD1 in laryngeal carcinomas[J]. Laryngoscope, 2008, 118(11): 1976-1980.
  6. 6. 　Tsai-Turton M, Luong BT, Tan Y, et al. Cyclophosphamide-induced apoptosis in COV434 human granulose cells involves oxidative stress and glutathione depletion[J]. Toxicol Sci, 2007, 98(1): 216-230.
  7. 7. 　Bilalovic N, Vranic S, Basic H, et al. Immunohistochemical evaluation of CyclinD1 in breast cancer[J]. Croat Med J, 2005, 46(3): 382-384.
  8. 8. 　Zhu SC, Li R, Wang YX, et al. Impact of simultaneous assay, the PCNA, cyclinD1, and DNA content with specimens before and after preoperative radiotherapy on prognosis of esophageal cancer possible incorporation into clinical TNM staging system[J]. World J Gastroenterol, 2005, 11(25): 3823-3829.
  9. 9. 　胡玉林, 劉志輝, 趙于天, 等. P53和CyclinD1蛋白的表達(dá)對(duì)食管癌預(yù)后意義的研究[J]. 哈爾濱醫(yī)科大學(xué)學(xué)報(bào), 2007, 41(1): 52-54.
  10. 10. 　金春亭, 張林西, 李海軍, 等. 人食管鱗癌中細(xì)胞周期調(diào)節(jié)基因蛋白的表達(dá)[J]. 世界華人消化雜志, 2005, 13(15): 1909-1912.
  11. 11. 　Fu ZJ, Ma ZY, Wang QR, et al. Overexpression of CyclinD1 and underexpression of p16 correlate with lymph node metastases in laryngeal squamous cell carcinoma in Chinese patients[J]. Clin Exp Metastasis, 2008, 25(8): 887-892.
  12. 12. 　Bahnassy AA, Zekri AR, Houssinis EL, et al. CyclinA and cyclinD, as significant prognostic markers in colorectal patients[J]. BMC Gastroenteral, 2004, 4(1): 22.
  13. 13. 　田冬艷, 王德華, 劉永紅, 等. Survivin和CyclinD1在卵巢癌中的表達(dá)和相關(guān)性[J]. 天津醫(yī)科大學(xué)學(xué)報(bào), 2009, 15(1): 81-87.
  14. 14. 　Shao J, Teraishi F, Katsuda K, et al. p53 inhibits adriamycin-induced down-regulation of cyclin D1 expression in human cancer cells[J]. Biochem Biophys Res Commun, 2002, 290(3): 1101-1107.