• 四川大學(xué)華西第二醫(yī)院婦產(chǎn)科，四川成都 610041;

目的：分析體外受精(IVF)患者卵泡液中sFas和sFasL的濃度與妊娠的關(guān)系，以及與卵泡液中的促黃體素(LH)水平之間的相關(guān)性。方法：收集行IVF/ICSI患者的卵泡液，測定sFas、sFasL和LH濃度。結(jié)果：妊娠組排卵前卵泡液中的sFasL和LH水平顯著高于未妊娠組(P分別為0.002和＜0.001)，兩組中sFas水平?jīng)]有明顯差異。通過logistic回歸分析，卵泡液中與妊娠有統(tǒng)計(jì)學(xué)相關(guān)的因素僅為LH，OR=4.117(P為0.001)。卵泡液中的LH和sFasL的水平呈明顯正相關(guān)關(guān)系(P lt;0.001)，LH與sFas之間無顯著相關(guān)關(guān)系。結(jié)論：在IVF/ICSI治療周期中，卵泡液中的sFasL蛋白和LH水平與妊娠結(jié)局有關(guān)，卵泡液中的LH水平可能通過調(diào)節(jié)卵泡局部的某些系統(tǒng)來調(diào)節(jié)卵泡中FasFasL系統(tǒng)介導(dǎo)的凋亡作用。

引用本文： 馬黔紅,李尚為,彭芝蘭,黃仲英. 卵泡液中游離Fas(sFas)、游離FasL(sFasL)以及LH的不同濃度對體外受精胚胎移植中妊娠結(jié)局的影響. 華西醫(yī)學(xué), 2009, 24(2): 319-322. doi: 復(fù)制

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2. Srivastava M，Lippes J，F(xiàn)ichorova R，et al.Soluble Fas(sFas)and Soluble Fas ligand(sFasL)in human reproductive tract fluid[M].IN：Processings of the 45th Annual Meeting of the Society for Gynecologic Investigation；Chicago，IL.Abstract T203..
3. de los Santos MJ，Anderson DJ，Racowsky C et al.Presence of FasFas ligand system and Bcl2 gene products in cells and fluids from gonadotropinstimulated human ovaries[J].Biol Reprod，2000，63：1811-1816..
4. 馬黔紅，李尚為，黃仲英，等.長效促性腺激素釋放激素激動(dòng)劑用于體外受精胚胎移植中降調(diào)節(jié)后延遲卵巢啟動(dòng)時(shí)間的探討[J].實(shí)用婦產(chǎn)科雜志，2006，22：603-606..
5. Kayagaki N，Kawasaki A，Ebata T，et al.Metalloproteinasemediated release of human Fas ligand[J].J Exp Med，1995，192：1777-1783..
6. Cataldo NA，F(xiàn)ujimoto VY，Jaffe RB.Interferongamma and activin A promote insulinlike growth factorbinding protein2 and 4 accumulation by human luteinizing granulosa cells，and interferongamma promotes their apoptosis[J].J Clin Endocrinol Metab，1998，83：179-186..
7. Quirk SM，Cowan RG，Joshi SG，Henrikson KP.Fas antigenmediated apoptosis in human granulosa/luteal cells[J].Biol Reprod，1995，52：279-287..
8. Porter DA，Harman RM，Cowan RG，et al.Susceptibility of ovarian granulosa cells to apoptosis differs in cells isolated before or after the preovulatory LH surge[J].Mol Cell Endocrinol，2001，15：13-20..
9. Westergaard LG，Erb K，Laursen SB，et al.Concentrations of gonadotropins and steroids in preovulatory follicular fluid and serum in relation to stimulation protocol and outcome of assisted reproduction treatment[J].RBMonline，2004，8：516-523..
10. Slot KA，de BoerBrouwer M，Houweling M et al.Luteinizing hormone inhibits Fasinduced apoptosis in ovarian surface epithelial cell lines[J].J Endocrinol，2006，188：227-239.
11. Chen Q，Yano T，Matsumi H，et al.Crosstalk between Fas/Fas ligand system and nitric oxide in the pathway subserving granulosa cell apoptosis：a possible regulatory mechanism for ovarian atresia[J].Endocrinology，2005，146：808-815.
  1. 1.
  2. 2. Srivastava M，Lippes J，F(xiàn)ichorova R，et al.Soluble Fas(sFas)and Soluble Fas ligand(sFasL)in human reproductive tract fluid[M].IN：Processings of the 45th Annual Meeting of the Society for Gynecologic Investigation；Chicago，IL.Abstract T203..
  3. 3. de los Santos MJ，Anderson DJ，Racowsky C et al.Presence of FasFas ligand system and Bcl2 gene products in cells and fluids from gonadotropinstimulated human ovaries[J].Biol Reprod，2000，63：1811-1816..
  4. 4. 馬黔紅，李尚為，黃仲英，等.長效促性腺激素釋放激素激動(dòng)劑用于體外受精胚胎移植中降調(diào)節(jié)后延遲卵巢啟動(dòng)時(shí)間的探討[J].實(shí)用婦產(chǎn)科雜志，2006，22：603-606..
  5. 5. Kayagaki N，Kawasaki A，Ebata T，et al.Metalloproteinasemediated release of human Fas ligand[J].J Exp Med，1995，192：1777-1783..
  6. 6. Cataldo NA，F(xiàn)ujimoto VY，Jaffe RB.Interferongamma and activin A promote insulinlike growth factorbinding protein2 and 4 accumulation by human luteinizing granulosa cells，and interferongamma promotes their apoptosis[J].J Clin Endocrinol Metab，1998，83：179-186..
  7. 7. Quirk SM，Cowan RG，Joshi SG，Henrikson KP.Fas antigenmediated apoptosis in human granulosa/luteal cells[J].Biol Reprod，1995，52：279-287..
  8. 8. Porter DA，Harman RM，Cowan RG，et al.Susceptibility of ovarian granulosa cells to apoptosis differs in cells isolated before or after the preovulatory LH surge[J].Mol Cell Endocrinol，2001，15：13-20..
  9. 9. Westergaard LG，Erb K，Laursen SB，et al.Concentrations of gonadotropins and steroids in preovulatory follicular fluid and serum in relation to stimulation protocol and outcome of assisted reproduction treatment[J].RBMonline，2004，8：516-523..
  10. 10. Slot KA，de BoerBrouwer M，Houweling M et al.Luteinizing hormone inhibits Fasinduced apoptosis in ovarian surface epithelial cell lines[J].J Endocrinol，2006，188：227-239.
  11. 11. Chen Q，Yano T，Matsumi H，et al.Crosstalk between Fas/Fas ligand system and nitric oxide in the pathway subserving granulosa cell apoptosis：a possible regulatory mechanism for ovarian atresia[J].Endocrinology，2005，146：808-815.