• 四川大學(xué)華西醫(yī)院 血液科、血液病研究室,四川成都 610041;

目的:研究丹參酮ⅡA(Tan ⅡA)對(duì)急性早幼粒細(xì)胞白血病(APL)細(xì)胞株NB4細(xì)胞誘導(dǎo)的血管內(nèi)皮細(xì)胞株(ECV304)促凝活性(PCA)的影響,并對(duì)其機(jī)制作初步探討。方法:(1)分別用1.0μg/mL TanⅡA、0.3μg/mLATRA、0.01%DMSO、PRMI1640處理NB4細(xì)胞24、48和72h,取其上清液作為條件培養(yǎng)基(hNB4-CM)。將這些CM分別與ECV304細(xì)胞在37oC共同孵育0、4、8和12h,用反復(fù)凍融法制備ECV304細(xì)胞裂解液,采用一期凝血法測(cè)定其PCA;采用ELISA法測(cè)定條件培養(yǎng)基中的TNF-α 。(2)ECV304細(xì)胞與1.0μg/mL TanⅡA及TanⅡA 72h-NB4-CM 在37oC共同分別孵育6、12、24和48h,并以ATRA和DMSO分別作為陽性和陰性對(duì)照,用上述相同方法測(cè)定ECV304細(xì)胞裂解液的PCA。結(jié)果:(1)1.0 μg/mL Tan ⅡA可以誘導(dǎo)NB4細(xì)胞分化,其作用NB4細(xì)胞的培養(yǎng)基有一定的升高ECV304細(xì)胞PCA的作用,該作用在孵育4h時(shí)達(dá)高峰,之后ECV304細(xì)胞PCA逐漸下降。與0.3μg/mL ATRA的作用無統(tǒng)計(jì)學(xué)差異(P gt;0.05)。(2)1.0 μg/mL的TanⅡA對(duì)TanⅡA72h-NB4-CM促ECV304細(xì)胞PCA有抑制作用,其強(qiáng)度隨作用時(shí)間增加而增加,與1.0μmol/L ATRA比較,P gt;0.05。(3)TanⅡA作用NB4細(xì)胞的培養(yǎng)基中TNF-α濃度,在作用前7h內(nèi)隨作用時(shí)間增加而增加,與0.3μg/mL ATRA比較無差異(P gt;0.05)。結(jié)論:Tan ⅡA能誘導(dǎo)NB4細(xì)胞分化,后者在分化過程中釋放的TNF-α可能與ECV304細(xì)胞PCA活性升高有關(guān);Tan-ⅡA又能抑制Tan-ⅡA-NB4-CM增強(qiáng)ECV304細(xì)胞PCA的作用。

引用本文： 代利霞,羊裔明,孟文彤. 丹參酮ⅡA對(duì)NB4所誘導(dǎo)的ECV304細(xì)胞促凝活性影響的機(jī)制探討. 華西醫(yī)學(xué), 2009, 24(3): 654-657. doi: 復(fù)制

1.
2. SHEBUSKI RJ,KILGORE KS.Role of inflammatory mediators in thrombogenesis[J].Journal of Pharmacology & Expermental Therapeutics,2002,300(3):729-735..
3. MECHTCHERIAKOVA D,SCHABBAUER G,LUCERNA M.Specificity,diversity,and convergence in V EGF and TNFalpha signaling events leading to tissue factor upregulation via EGR1 in endothelial cells[J].FASEB Journal,2001,15(1):230-242..
4. 梁勇,羊裔明,袁淑蘭,等.丹參酮IIA 誘導(dǎo)急性早幼粒細(xì)胞白血病細(xì)胞分化及其分子機(jī)制研究[J].中華血液學(xué)雜志,2000,21(1):22-26..
5. 程韻楓,羊裔明,鄧承祺.丹參酮IIA 對(duì)NB4 細(xì)胞促凝活性的影響[J].華西醫(yī)科大學(xué)學(xué)報(bào),2002,33(4):553-555..
6. 張宏麗,羊裔明,孟文彤等.丹參酮IIA對(duì)NB4 所誘導(dǎo)的人ECV304細(xì)胞株促凝活性的影響[J].四川大學(xué)學(xué)報(bào)(醫(yī)學(xué)版),2006,37(1):55-59..
7. FALANGA A,MARCHETTI M,GIOVANELLI S,et al.Alltransretinoic acid counteracts endothelial cell procoagulant activity induced by a human promyelocytic leukemiaderived cell line(NB4)[J].Blood,1996,87(2):613-617..
8. SOIGNET SL,FRANKEL SR,DOUER D,et al.United States multicenter study of arsenic trioxide in relapsed acute promyelocytic leukemia[J].J Clin Oncol,2001,19(18):3852-3860..
  1. 1.
  2. 2. SHEBUSKI RJ,KILGORE KS.Role of inflammatory mediators in thrombogenesis[J].Journal of Pharmacology & Expermental Therapeutics,2002,300(3):729-735..
  3. 3. MECHTCHERIAKOVA D,SCHABBAUER G,LUCERNA M.Specificity,diversity,and convergence in V EGF and TNFalpha signaling events leading to tissue factor upregulation via EGR1 in endothelial cells[J].FASEB Journal,2001,15(1):230-242..
  4. 4. 梁勇,羊裔明,袁淑蘭,等.丹參酮IIA 誘導(dǎo)急性早幼粒細(xì)胞白血病細(xì)胞分化及其分子機(jī)制研究[J].中華血液學(xué)雜志,2000,21(1):22-26..
  5. 5. 程韻楓,羊裔明,鄧承祺.丹參酮IIA 對(duì)NB4 細(xì)胞促凝活性的影響[J].華西醫(yī)科大學(xué)學(xué)報(bào),2002,33(4):553-555..
  6. 6. 張宏麗,羊裔明,孟文彤等.丹參酮IIA對(duì)NB4 所誘導(dǎo)的人ECV304細(xì)胞株促凝活性的影響[J].四川大學(xué)學(xué)報(bào)(醫(yī)學(xué)版),2006,37(1):55-59..
  7. 7. FALANGA A,MARCHETTI M,GIOVANELLI S,et al.Alltransretinoic acid counteracts endothelial cell procoagulant activity induced by a human promyelocytic leukemiaderived cell line(NB4)[J].Blood,1996,87(2):613-617..
  8. 8. SOIGNET SL,FRANKEL SR,DOUER D,et al.United States multicenter study of arsenic trioxide in relapsed acute promyelocytic leukemia[J].J Clin Oncol,2001,19(18):3852-3860..