目的:評(píng)價(jià)免疫印跡法檢測(cè)胰島自身抗體（GAD-A、ICA、IAA）與酶聯(lián)免疫法測(cè)ICA、GAD-A放射免疫法測(cè)IAA結(jié)果的一致性。方法:采用免疫印跡法測(cè)定81例糖尿病患者胰島自身抗體,將結(jié)果與酶聯(lián)免疫法測(cè)定的GAD-A、ICA,放射免疫法測(cè)定IAA結(jié)果進(jìn)行比較。結(jié)果:免疫印跡法陽(yáng)性檢出率為:GAD-A 51.8%,ICA 18.5%,IAA 27.1%；酶聯(lián)免疫法（GAD-A、ICA）、放射免疫法（IAA）陽(yáng)性檢出率:GAD-A 32.1%,ICA 34.5%,IAA 30.8%；上述兩組結(jié)果進(jìn)行比較,兩組相比ICA和GAD-A有統(tǒng)計(jì)學(xué)差異（P lt;0.05）,IAA無(wú)統(tǒng)計(jì)學(xué)差異。兩組結(jié)果一致率比較:GAD-A 50.6%,ICA 64.2%,IAA 69.1%。結(jié)論:與臨床常用酶聯(lián)免疫法檢測(cè)GAD-A、ICA,放射免疫法檢測(cè)IAA比較,免疫印跡法和酶聯(lián)免疫法在ICA及GAD-A陽(yáng)性檢出率上的差異有顯著性,和放射免疫法在IAA陽(yáng)性檢出率上差異無(wú)顯著性。
引用本文： 張宇,陳玲,熊中云,安振梅. 免疫印跡法、酶聯(lián)免疫法及放射免疫法對(duì)胰島自身抗體檢測(cè)的比較. 華西醫(yī)學(xué), 2009, 24(3): 697-699. doi: 復(fù)制
| 1. | VERGE C F, STERNGER D, BONIFACIO E, et al. Combined use of autoantibodies（IA2 autoantibody, GAD antibody, insulin autoantibody, cytoplasmic islet cell antibodies）in type 1 diabetes: Combinational Islet Autoantibody Workshop [J]. Diabetes,1998,47（5）:1857-1866.. |
| 2. | YAMAMOTO A M, DESCHAMPS I, GARCHON H J, et al. Young age and HLA markers enhance the risk of progression to type 1 diabetes in antibodypositive siblings of diabetic children [J]. J Autoimmun,1998,11（6）:643-650.. |
| 3. | 鄧智勇,劉英,安振梅.三聯(lián)蛋白芯片法在胰島自身抗體檢測(cè)中的臨床應(yīng)用[J].華西醫(yī)學(xué),2006,21（1）:92-93.. |
| 4. | 張慶俠,郝建華,胡鋒蘭,等.免疫印跡法檢測(cè)糖尿病患者自身抗體的臨床應(yīng)用[J].實(shí)用醫(yī)學(xué)雜志,2007,23（13）:2093-2094.. |
| 5. | 張慶俠,郝建華,程瓊,等.3種方法檢測(cè)胰島素自身抗體的結(jié)果比較[J].實(shí)用全科醫(yī)學(xué),2008,6（5）:526-527.. |
| 6. | TOM C, LANDINOLSSON M, LERNAMARK A, et al. Prognostic factors for the course of β cell function in autoimmune diabetes[J]. Clin Endocrinol Metab,2000,85（12）:4619-4623.. |
| 7. | TUOMI T, GROOP L C, ZIMMET P Z, et al. Antibodies to glutamic acid decarboxylase reveal latent autoimmune diabetes mellitus in adults with a noninsulindependent onset of disease [J].Diabetes,1993,42（2）:359-362.. |
- 1. VERGE C F, STERNGER D, BONIFACIO E, et al. Combined use of autoantibodies（IA2 autoantibody, GAD antibody, insulin autoantibody, cytoplasmic islet cell antibodies）in type 1 diabetes: Combinational Islet Autoantibody Workshop [J]. Diabetes,1998,47（5）:1857-1866..
- 2. YAMAMOTO A M, DESCHAMPS I, GARCHON H J, et al. Young age and HLA markers enhance the risk of progression to type 1 diabetes in antibodypositive siblings of diabetic children [J]. J Autoimmun,1998,11（6）:643-650..
- 3. 鄧智勇,劉英,安振梅.三聯(lián)蛋白芯片法在胰島自身抗體檢測(cè)中的臨床應(yīng)用[J].華西醫(yī)學(xué),2006,21（1）:92-93..
- 4. 張慶俠,郝建華,胡鋒蘭,等.免疫印跡法檢測(cè)糖尿病患者自身抗體的臨床應(yīng)用[J].實(shí)用醫(yī)學(xué)雜志,2007,23（13）:2093-2094..
- 5. 張慶俠,郝建華,程瓊,等.3種方法檢測(cè)胰島素自身抗體的結(jié)果比較[J].實(shí)用全科醫(yī)學(xué),2008,6（5）:526-527..
- 6. TOM C, LANDINOLSSON M, LERNAMARK A, et al. Prognostic factors for the course of β cell function in autoimmune diabetes[J]. Clin Endocrinol Metab,2000,85（12）:4619-4623..
- 7. TUOMI T, GROOP L C, ZIMMET P Z, et al. Antibodies to glutamic acid decarboxylase reveal latent autoimmune diabetes mellitus in adults with a noninsulindependent onset of disease [J].Diabetes,1993,42（2）:359-362..