• 四川大學(xué)華西醫(yī)院生物治療國(guó)家重點(diǎn)實(shí)驗(yàn)室,四川成都 610041;

目的:探討雌激素饑餓對(duì)腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體(TRAIL)誘導(dǎo)乳腺癌細(xì)胞MCF-7凋亡的影響及作用機(jī)制?！糎TH〗方法〖HTSS〗:MCF-7細(xì)胞培養(yǎng)貼壁之后,用雌激素饑餓處理后加入TRAIL,用熒光染料Hoechst染色法檢測(cè)細(xì)胞的凋亡,用細(xì)胞計(jì)數(shù)法檢測(cè)細(xì)胞的存活。在雌激素饑餓處理MCF-7細(xì)胞后,收集對(duì)照和饑餓組蛋白用Western blot法檢測(cè)相關(guān)的蛋白表達(dá)?！糎TH〗結(jié)果〖HTSS〗:單獨(dú)使用雌激素饑餓處理或者單獨(dú)使用TRAIL處理乳腺癌細(xì)胞MCF-7都能誘導(dǎo)細(xì)胞凋亡,但是它們誘導(dǎo)凋亡的活性較小,兩種方法聯(lián)合使用可以極大地增加誘導(dǎo)細(xì)胞凋亡的活性(P lt;0.001)。雌激素饑餓處理后的乳腺癌細(xì)胞,死亡受體5(DR5)表達(dá)上調(diào)?！糎TH〗結(jié)論〖HTSS〗:乳腺癌細(xì)胞MCF-7對(duì)TRAIL敏感度不高,雌激素饑餓可以增加TRAIL誘導(dǎo)乳腺癌細(xì)胞凋亡的活性,DR5與雌激素饑餓誘導(dǎo)TRAIL活性增加相關(guān)。

引用本文： 汪冉冉,劉東波,羅婷. 雌激素饑餓增強(qiáng)TRAIL誘導(dǎo)乳腺癌細(xì)胞凋亡. 華西醫(yī)學(xué), 2009, 24(4): 923-925. doi: 復(fù)制

1. TRAVIS R C, KEY T J. Oestrogen exposure and breast cancer risk[J]. Breast Cancer Res,2003,5(5):239-247..
2. PIKE M C, SPICER D V, DAHMOUSH L, et al. Estrogens, progestogens, normal breast cell proliferation, and breast cancer risk[J]. Epidemiol Rev,1993,15(1):17-20..
3. 楊才明, 夏淑君.HER2 2/neu基因蛋白、BRCA1和BRCA2基因突變檢測(cè)在乳腺癌診斷和治療中的臨床意義[J].標(biāo)記免疫分析與臨床,2005,12(1):43-45..
4. WILEY S R, SCHOOLEY K, SMOLAK P J, et al. Indentification and characterization of a new member of the TNF family that induces apoptosis[J]. Immunity,1995,3(6):673-682..
5. ASHKENAZI A, PAI R C, FONG S, et al. Safety and antitumor activity of recombinant soluble Apo2 ligand[J]. J Clin Invest,1999,104(2):155-162..
6. 張玉松,傅晉祥,周麗英,等.腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體與赫賽汀聯(lián)用對(duì)C-erbB2過(guò)表達(dá)的乳腺癌SKBR3細(xì)胞株的影響[J]. 蘇州大學(xué)學(xué)報(bào),2007,27(1):38-40..
7. YAGER J D, DAVIDSON N E. Estrogen carcinogenesis in breast cancer[J]. N Engl J Med,2006,354:270-282..
8. SAUNDERS P T, MILLAR M R, WILLIAMS K, et al. Expression of oestrogen receptor β (ERβ1) protein in human breast cancer biopsies[J]. Br J Cancer,2002,86(2):250-256..
9. 朱冉旭, 林菊生.死亡受體DR5與腫瘤細(xì)胞凋亡[J].世界華人消化雜志,2004,12(8):1909-1912.
10. 馬遠(yuǎn)方, 張軍, 趙粵萍, 等.腫瘤細(xì)胞對(duì)TRAIL敏感性與其表面DR5表達(dá)水平的相關(guān)研究[J].中華腫瘤雜志,2004,26(9):528-530..
11. 李妍,曹云新,張赟,等.IFN-α對(duì)腫瘤細(xì)胞上TRAIL受體 DR4 和DR5 表達(dá)的調(diào)節(jié)及意義[J].第四軍醫(yī)大學(xué)吉林軍醫(yī)學(xué)院學(xué)報(bào),2005,26(1):7-10.
  1. 1. TRAVIS R C, KEY T J. Oestrogen exposure and breast cancer risk[J]. Breast Cancer Res,2003,5(5):239-247..
  2. 2. PIKE M C, SPICER D V, DAHMOUSH L, et al. Estrogens, progestogens, normal breast cell proliferation, and breast cancer risk[J]. Epidemiol Rev,1993,15(1):17-20..
  3. 3. 楊才明, 夏淑君.HER2 2/neu基因蛋白、BRCA1和BRCA2基因突變檢測(cè)在乳腺癌診斷和治療中的臨床意義[J].標(biāo)記免疫分析與臨床,2005,12(1):43-45..
  4. 4. WILEY S R, SCHOOLEY K, SMOLAK P J, et al. Indentification and characterization of a new member of the TNF family that induces apoptosis[J]. Immunity,1995,3(6):673-682..
  5. 5. ASHKENAZI A, PAI R C, FONG S, et al. Safety and antitumor activity of recombinant soluble Apo2 ligand[J]. J Clin Invest,1999,104(2):155-162..
  6. 6. 張玉松,傅晉祥,周麗英,等.腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體與赫賽汀聯(lián)用對(duì)C-erbB2過(guò)表達(dá)的乳腺癌SKBR3細(xì)胞株的影響[J]. 蘇州大學(xué)學(xué)報(bào),2007,27(1):38-40..
  7. 7. YAGER J D, DAVIDSON N E. Estrogen carcinogenesis in breast cancer[J]. N Engl J Med,2006,354:270-282..
  8. 8. SAUNDERS P T, MILLAR M R, WILLIAMS K, et al. Expression of oestrogen receptor β (ERβ1) protein in human breast cancer biopsies[J]. Br J Cancer,2002,86(2):250-256..
  9. 9. 朱冉旭, 林菊生.死亡受體DR5與腫瘤細(xì)胞凋亡[J].世界華人消化雜志,2004,12(8):1909-1912.
  10. 10. 馬遠(yuǎn)方, 張軍, 趙粵萍, 等.腫瘤細(xì)胞對(duì)TRAIL敏感性與其表面DR5表達(dá)水平的相關(guān)研究[J].中華腫瘤雜志,2004,26(9):528-530..
  11. 11. 李妍,曹云新,張赟,等.IFN-α對(duì)腫瘤細(xì)胞上TRAIL受體 DR4 和DR5 表達(dá)的調(diào)節(jié)及意義[J].第四軍醫(yī)大學(xué)吉林軍醫(yī)學(xué)院學(xué)報(bào),2005,26(1):7-10.