• 四川大學華西醫(yī)院生物治療國家重點實驗室,四川成都 610041;

目的:探討表沒食子兒茶素沒食子酸酯(EGCG)對乳腺癌細胞MCF7生長的影響及對乳腺癌細胞MDAMB231遷移的影響。方法:MCF7細胞培養(yǎng)貼壁之后,加入EGCG處理,2d后收集蛋白,采用Western Blot檢測磷酸化p38絲裂原活化蛋白激酶(phosphop38MAPK)的表達;同樣處理后收集活細胞,用細胞計數(shù)法檢測細胞的存活;取對數(shù)生長期的MDAMB231細胞,分至6孔板培養(yǎng),使用EGCG處理后,采用細胞劃線法探測乳腺癌細胞的遷移。結(jié)果:使用EGCG處理乳腺癌細胞后,phosphop38MAPK的表達降低,EGCG處理乳腺癌細胞4d后其增殖率降低50%,遷移活性降低。結(jié)論:EGCG處理乳腺癌細胞能抑制腫瘤細胞的生長以及遷移,這與p38MAPK信號通路相關(guān)。

引用本文： 劉東波,汪冉冉,羅婷. 表沒食子兒茶素沒食子酸酯對乳腺癌細胞生長及遷移的影響. 華西醫(yī)學, 2009, 24(5): 1185-1188. doi: 復制

1. 朱發(fā)偉.茶葉中表沒食子兒茶素沒食子酸酯的研究進展[J].中草藥,2005,36(8):12711171---1272.
2. 李奕.表沒食子兒茶素沒食子酸酯抗白血病的分子機制[J].國外醫(yī)學醫(yī)學地理分冊,2004,25(4):178181.
3. SAEKI K, KOBAYASHI N, INAZAWA Y, et al. Oxidationtriggered cJun Nterminal kinase (JNK) and p38 mitogenactivated protein (MAP) kinase pathways for apoptosis in human leukaemic cells stimulated by epigallocatechin3gallate (EGCG): a distinct pathway from those of chemically inducedand receptormediated apoptosis [J]. Biochem J,2002,368(3):705720.
4. HUANG S, NEW L, PAN Z X, et al. Urokinase plasminogen activator/urokinase specific surface receptor expression and matrix invasion by breast cancer cells requires constitutive p38 mitogenactivated protein kinase activity[J]. J Biol Chem,2000,275(16):1226612272.
5. 李鶴成,陸勁松,侯意楓,等.EGCG對人乳腺癌MDAMB435細胞株細胞增殖的抑制及機制[J].中國癌癥雜志,2004,14(1):911.
6. LEA M A, XIAO Q, SADHUKHAN A K, et al. Inhibitory action of tea extracts and()epigallocatechin gallate on cell proliferation[J]. Proc Annu Meet Am Assoc Cancer Res,1993,34(4):771.
7. HASTAK K, GUPTA S, AHMAD N, et al. Role of p53 and NFκB in epigallocatechin3gallateinduced apoptosis of LNCaP cells[J]. Oncogene,2003,22(31):48514859.
8. 余艷輝,丁仁奎,張志偉,等.酯型兒茶素單體EGCG和GCG對人腸癌SW480細胞生長抑制作用及其機制[J].中國醫(yī)師雜志,2005,7(2):161163.
9. 譚曉華,張亞歷,周殿元.EGCG誘導胃癌和肝癌細胞凋亡及bcl2表達下調(diào)的研究[J].癌癥,2000,19(17):638641.
10. 譚曉華,周殿元,張亞歷,等.沒食子兒茶素沒食子酸酯誘導大腸癌LoVo細胞凋亡和凋亡相關(guān)基因表達的改變[J].中華醫(yī)學雜志,1999,79(4):308310.
11. CHUNG J Y, PARK J O, PHYU H P, et al. Mechanisms of inhibition of the rasMAP kinase signaling pathway in 307b ras 12 cells by tea polyphenols()epigallocatechin3gallate and theaflavin3,3′digallate[J]. FASEB J,2001,15(11):20222024..
12. CHOI Y J, LIM S Y, WOO J H, et al. Sodium orthovanadate potentiates EGCGinduced apoptosis that is dependent on the Erk pathway[J]. Biochem Biophys Res Commun,2003,305(1):176185.
  1. 1. 朱發(fā)偉.茶葉中表沒食子兒茶素沒食子酸酯的研究進展[J].中草藥,2005,36(8):12711171---1272.
  2. 2. 李奕.表沒食子兒茶素沒食子酸酯抗白血病的分子機制[J].國外醫(yī)學醫(yī)學地理分冊,2004,25(4):178181.
  3. 3. SAEKI K, KOBAYASHI N, INAZAWA Y, et al. Oxidationtriggered cJun Nterminal kinase (JNK) and p38 mitogenactivated protein (MAP) kinase pathways for apoptosis in human leukaemic cells stimulated by epigallocatechin3gallate (EGCG): a distinct pathway from those of chemically inducedand receptormediated apoptosis [J]. Biochem J,2002,368(3):705720.
  4. 4. HUANG S, NEW L, PAN Z X, et al. Urokinase plasminogen activator/urokinase specific surface receptor expression and matrix invasion by breast cancer cells requires constitutive p38 mitogenactivated protein kinase activity[J]. J Biol Chem,2000,275(16):1226612272.
  5. 5. 李鶴成,陸勁松,侯意楓,等.EGCG對人乳腺癌MDAMB435細胞株細胞增殖的抑制及機制[J].中國癌癥雜志,2004,14(1):911.
  6. 6. LEA M A, XIAO Q, SADHUKHAN A K, et al. Inhibitory action of tea extracts and()epigallocatechin gallate on cell proliferation[J]. Proc Annu Meet Am Assoc Cancer Res,1993,34(4):771.
  7. 7. HASTAK K, GUPTA S, AHMAD N, et al. Role of p53 and NFκB in epigallocatechin3gallateinduced apoptosis of LNCaP cells[J]. Oncogene,2003,22(31):48514859.
  8. 8. 余艷輝,丁仁奎,張志偉,等.酯型兒茶素單體EGCG和GCG對人腸癌SW480細胞生長抑制作用及其機制[J].中國醫(yī)師雜志,2005,7(2):161163.
  9. 9. 譚曉華,張亞歷,周殿元.EGCG誘導胃癌和肝癌細胞凋亡及bcl2表達下調(diào)的研究[J].癌癥,2000,19(17):638641.
  10. 10. 譚曉華,周殿元,張亞歷,等.沒食子兒茶素沒食子酸酯誘導大腸癌LoVo細胞凋亡和凋亡相關(guān)基因表達的改變[J].中華醫(yī)學雜志,1999,79(4):308310.
  11. 11. CHUNG J Y, PARK J O, PHYU H P, et al. Mechanisms of inhibition of the rasMAP kinase signaling pathway in 307b ras 12 cells by tea polyphenols()epigallocatechin3gallate and theaflavin3,3′digallate[J]. FASEB J,2001,15(11):20222024..
  12. 12. CHOI Y J, LIM S Y, WOO J H, et al. Sodium orthovanadate potentiates EGCGinduced apoptosis that is dependent on the Erk pathway[J]. Biochem Biophys Res Commun,2003,305(1):176185.