目的:探討白藜蘆醇對人腦膠質(zhì)瘤U251細胞的凋亡相關(guān)因子caspase-3的作用。方法:應(yīng)用RT-PCR方法和Western-blot分別檢測凋亡因子caspase-3的RNA和蛋白水平的表達。結(jié)果:白藜蘆醇能明顯促進U251細胞的凋亡相關(guān)因子caspase-3的表達,存在劑量依賴與時間依賴性。結(jié)論:白藜蘆醇能通過caspase－3途徑誘導(dǎo)U251細胞的凋亡。
引用本文： 陳忠勇,彭強. 白藜蘆醇對人腦膠質(zhì)瘤U251細胞中凋亡相關(guān)因子Caspase-3的表達影響. 華西醫(yī)學(xué), 2009, 24(8): 2139-2141. doi: 復(fù)制
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- 2. OURIA M, GUKOVSKAYA A, JUNG Y, et al. Foodderived polyphenols inhibit pancrentic cancer growth through mitochondrial cytochrome C release and apoptosis[J]. Int J Cancer,2002,8(5):767-769.
- 3. ATHAR M, BACK JH, TANG X, et al. Resveratrol: a review of p re2clinical studies for human cancer prevention[J]. Toxicol Appl Pharmacol, 2007, 224 (3):274-283.
- 4. AZIOSNG, KRISHNAMOORTHY L, HARRISM, et al. Estrogen and resveratrol regulate Rac and Cdc42 signaling to the sctin cytoskeleton of metastatic breast cancer cells[J]. Neoplasia, 2007, 9 (2): 147-158.
- 5. FULDA S, DEBATIN KM. Resveratrolmediated sensitisation to TRAILinduced apoptosis depends on death receptor and mitochondrial signaling[J]. Fur J Cancer, 2005, 41 (5): 786-798.
- 6. RIGOLIO R, MILOSOM, NICOLINI G,et al. Resveratrol interference with the cell cycle protects human neuroblastoma SHSY5Y cell from paclitaxel induced apoptosis[J]. Neurochem Int, 2005, 46 (3): 205-211.
- 7. HORN TL, CWIK MJ, MORRISSEY RL, et al. Oncogenicity evaluation of resveratrol in p53(+ /) (p53 knock-out) mice[J]. Food Chem Toxicol, 2007, 45 (1):55-63.
- 8. 王玉, 孫黎光, 夏春輝. Caspase介導(dǎo)的Fas凋亡途徑[J].世界華人消化雜志, 2006,14(36): 3439-3442.
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