• 1四川大學華西醫(yī)院急診科, 四川成都 610041;2南通大學醫(yī)學院病理教研室;

目的:研究非小細胞肺癌中PGSK-3β、CEA、C-myc的表達,探討它們與肺癌臨床病理因素的相關性及在肺癌中三指標之間的相關性。方法:運用免疫組化法檢測90例肺癌PGSK-3β、CEA、C-myc的表達情況。結果 肺腺癌PGSK-3β陽性率明顯高于鱗癌(P lt;0.05),臨床Ⅲ-Ⅳ期陽性率顯著高于Ⅰ-Ⅱ期(P lt;0.05)。周圍型肺癌CEA陽性率顯著高于中央型肺癌(P lt;0.01),有淋巴結轉移者陽性率顯著高于無淋巴結轉移者(P lt;0.05)。肺癌中、低分化組C-myc陽性率顯著高于高分化組(P lt;0.05);C-myc陽性強度表達呈拋物線狀趨勢,在中分化組顯著高于低分化組和高分化組。肺癌中PGSK-3β與CEA表達呈正相關(P lt;0.05)。 結論:PGSK-3β促進肺癌的演進過程,與肺癌的分化、分期相關。肺癌高表達CEA時癌組織異質性粘附增加,有利于其浸潤與轉移。肺癌中C-myc表達增加是肺癌發(fā)生中的常見分子事件,與癌細胞分化有關。肺癌中PGSK-3β與CEA表達呈正相關。

引用本文： 王革平,陳莉李信明,張逸,李定明,蔣鵬飛. 非小細胞肺癌中PGSK-3β、CEA、C-myc表達與臨床病理因素的相關性研究. 華西醫(yī)學, 2009, 24(9): 2320-2323. doi: 復制

1. JIA J H, AMANAI K, WANG G L, et al. Shaggy/GSK3 antagonizes Hedgehog signaling by regulating Cubitus interruptus[J]. Nature, 2002, 416(6880): 548 -552.
2. SOTOCERRATO V,VINALS F,LAMBERT JR,et al. Prodigiosin induces the proapoptotic gene NAG1 via glycogen synthase kinase3β activity in breast cancer cells[J]. Mol Cancer Ther, 2007, 6 (1): 362-369.
3. LI J, MIZUKAMI Y, ZHANG X, et al.Oncogenic Kras stimulates Wnt singaling in colon cancer through inhibition of GSK3[J]. J Gastroenterology, 2005, 128 (7):1907-1918.
4. LEI SA, DUBEYKOVSKIY A, CHAKLADAR L, et al. The murine gastrin promoter is synergistically activated by transforming growth factorβ/ SMAD and Wnt signaling pathways[J]. J Biol Chem, 2004, 279 (41): 42492-42502.
5. DING VW, CHEN RH,MCCORMICK FJ. Differential regulation of glycogen synthase kinase3βby insulin and wnt signaling[J]. Biol Chem, 2000, 275 (42): 32475-32481.
6. LIANG J, SLINGERLAND J M. Multiple roles of the PI3K/PKB (Akt) pathway in cell cycleprogression[J]. Cell Cycle, 2003, 2 (4):339-345.
7. ALT J R, CLEVELAND J L, HANNINK M, et al. Phosphorylation dependent regulation of cyclinD1 nuclear export and cyclinD1dependent cellular transformation[J]. Genes Dev, 2000,14 (24):3102-3114.
8. BAN KC, SINGH H, KRISHNAN R, et al. GSK3 β phosphorylation and alteration of βcatenin in hepatocellularcarcinoma[J]. Cancer Lett, 2003, 199 (2): 201-208.
9. GOTO H, KAWANO K, KOBAYASHI I, et al. Expression of cyclin D1 and GSK3β and their predictivevalue of prognosis in squamous cell carcinomas of the tongue[J].Oral Oncol, 2002, 38 (6): 549-556.
10. HA N C, TONOZUKA T, STAMOS J L, CHOI HJ, WEIS W I. Mechanism of phosphorylationdependent binding of APC to βcatenin and its role in βcatenin degradation[J]. Mol Cell, 2004, 15 (4): 511-521.
11. 王志新, 高明發(fā). 非小細胞肺癌癌胚抗原表達的臨床意義[J]. 第三軍醫(yī)大學學報, 2001, 23 (1): 102-104.
12. LU LX, JIANG HC, ZHU AL. Gene expression profiles in liver cancer and normal liver tissues[J]. World J Gastroenterol, 2000, 6 (suppl3): 85.
13. WANG Q, LIN ZY, FENG XL. Alterations in metastatic properties of hepat ocellular carcinoma cell following oncogene transfection[J].World J Gastroentero L, 2001, 7 (3): 335-339.
14. LI J, MIZUKAMI Y, ZHANG X, et al.Oncogenic Kras stimulates Wnt singaling in colon cancer through inhibition of GSK3β[J]. J Gastroenterology, 2005, 128 (7): 1907-1918.
15. XU AG, LI SG, LIU J H, et al.Function of apoptosis and expression of he proteins Bcl2, p53 and Cmyc in the development of gastric cancer[J]. World J Gastroenterol, 2001, 7 (3): 403406.
  1. 1. JIA J H, AMANAI K, WANG G L, et al. Shaggy/GSK3 antagonizes Hedgehog signaling by regulating Cubitus interruptus[J]. Nature, 2002, 416(6880): 548 -552.
  2. 2. SOTOCERRATO V,VINALS F,LAMBERT JR,et al. Prodigiosin induces the proapoptotic gene NAG1 via glycogen synthase kinase3β activity in breast cancer cells[J]. Mol Cancer Ther, 2007, 6 (1): 362-369.
  3. 3. LI J, MIZUKAMI Y, ZHANG X, et al.Oncogenic Kras stimulates Wnt singaling in colon cancer through inhibition of GSK3[J]. J Gastroenterology, 2005, 128 (7):1907-1918.
  4. 4. LEI SA, DUBEYKOVSKIY A, CHAKLADAR L, et al. The murine gastrin promoter is synergistically activated by transforming growth factorβ/ SMAD and Wnt signaling pathways[J]. J Biol Chem, 2004, 279 (41): 42492-42502.
  5. 5. DING VW, CHEN RH,MCCORMICK FJ. Differential regulation of glycogen synthase kinase3βby insulin and wnt signaling[J]. Biol Chem, 2000, 275 (42): 32475-32481.
  6. 6. LIANG J, SLINGERLAND J M. Multiple roles of the PI3K/PKB (Akt) pathway in cell cycleprogression[J]. Cell Cycle, 2003, 2 (4):339-345.
  7. 7. ALT J R, CLEVELAND J L, HANNINK M, et al. Phosphorylation dependent regulation of cyclinD1 nuclear export and cyclinD1dependent cellular transformation[J]. Genes Dev, 2000,14 (24):3102-3114.
  8. 8. BAN KC, SINGH H, KRISHNAN R, et al. GSK3 β phosphorylation and alteration of βcatenin in hepatocellularcarcinoma[J]. Cancer Lett, 2003, 199 (2): 201-208.
  9. 9. GOTO H, KAWANO K, KOBAYASHI I, et al. Expression of cyclin D1 and GSK3β and their predictivevalue of prognosis in squamous cell carcinomas of the tongue[J].Oral Oncol, 2002, 38 (6): 549-556.
  10. 10. HA N C, TONOZUKA T, STAMOS J L, CHOI HJ, WEIS W I. Mechanism of phosphorylationdependent binding of APC to βcatenin and its role in βcatenin degradation[J]. Mol Cell, 2004, 15 (4): 511-521.
  11. 11. 王志新, 高明發(fā). 非小細胞肺癌癌胚抗原表達的臨床意義[J]. 第三軍醫(yī)大學學報, 2001, 23 (1): 102-104.
  12. 12. LU LX, JIANG HC, ZHU AL. Gene expression profiles in liver cancer and normal liver tissues[J]. World J Gastroenterol, 2000, 6 (suppl3): 85.
  13. 13. WANG Q, LIN ZY, FENG XL. Alterations in metastatic properties of hepat ocellular carcinoma cell following oncogene transfection[J].World J Gastroentero L, 2001, 7 (3): 335-339.
  14. 14. LI J, MIZUKAMI Y, ZHANG X, et al.Oncogenic Kras stimulates Wnt singaling in colon cancer through inhibition of GSK3β[J]. J Gastroenterology, 2005, 128 (7): 1907-1918.
  15. 15. XU AG, LI SG, LIU J H, et al.Function of apoptosis and expression of he proteins Bcl2, p53 and Cmyc in the development of gastric cancer[J]. World J Gastroenterol, 2001, 7 (3): 403406.