摘要:目的: 探討選擇性內(nèi)皮素A受體拮抗劑BQ123對(duì)人喉癌Hep2細(xì)胞裸鼠種植瘤的生長(zhǎng)及血管形成的影響。 方法 :將實(shí)驗(yàn)動(dòng)物裸鼠隨機(jī)分為3組:BQ123［n =8,2mg/(kg·day)］、氟尿嘧啶組［n =8,2mg/(kg·day)］、生理鹽水組(n =8),比較各組裸鼠成瘤體積、微血管密度(MVD)。 結(jié)果 :BQ123組腫瘤體積為(162±053)cm3,明顯小于生理鹽水組及氟尿嘧啶組,差異具有統(tǒng)計(jì)學(xué)意義;BQ123組的腫瘤組織中MVD高倍鏡下為232,明顯低于生理鹽水組(586)及氟尿嘧啶組(395),差異具有統(tǒng)計(jì)學(xué)意義。 結(jié)論 :BQ123對(duì)人喉癌Hep2細(xì)胞在裸鼠體內(nèi)有明顯抑瘤作用,腫瘤的體積、腫瘤組織MVD顯著低于對(duì)照組,表明BQ123可通過抑制腫瘤血管生成而顯著抑制腫瘤生長(zhǎng)。
Abstract: Objective: To study the effects of endothelin A receptor blockade BQ123 on the implanted human laryngeal carcinoma angiogenesis of nude mouse. Methods : From March 2008 to July 2009, 24 Balb/c nude mice were randomly divided into three groups: BQ123 group ［〖WTBX〗n =8, BQ123 at 2mg/(kg·day)］, 5Fu group ［〖WTBX〗n =8, fluorouracil at 2mg/(kg·day)］ and the control group (〖WTBX〗n =8, normal saline). The carcinoma volume and microvascular density of each group were compared. Results : The tumor size of BQ123 group, which was (162±053)cm3 in average, was significant smaller than the tumor sizes of the other two group s. The average microvascular density score of the tumors in BQ123 group was 232 per hyper power len (HP), which was also significantly less than the average scores of control groups (586 and 395 respectively). Conclusion : Nude mouse experiments show that the carcinoma volume and microvascular density of BQ123 group are significantly lower than those of the control groups. BQ123 inhibits the growth of carcinoma by its inhibition of carcinoma angiogenesis.
引用本文： 林雁,張京晶,馮駿,劉世喜. 內(nèi)皮素A受體拮抗劑在人喉癌種植瘤的實(shí)驗(yàn)研究. 華西醫(yī)學(xué), 2009, 24(10): 2674-. doi: 復(fù)制