• 解放軍第251醫(yī)院病理科(河北張家口 075000);

　目的 　探討結(jié)直腸腺癌組織中Fascin-1蛋白表達(dá)及與其臨床病理參數(shù)的關(guān)系。
　方法 　采用微波-EliVisionTM免疫組化染色方法檢測(cè)60例結(jié)直腸腺癌、30例結(jié)直腸腺瘤及30例正常結(jié)直腸黏膜組織（遠(yuǎn)離癌組織4 cm）中Fascin-1蛋白的表達(dá)情況，并分析其與結(jié)直腸腺癌浸潤(rùn)、轉(zhuǎn)移等的關(guān)系。
　結(jié)果 　Fascin-1蛋白陽(yáng)性表達(dá)主要在細(xì)胞質(zhì),其在正常結(jié)直腸黏膜組織、結(jié)直腸腺瘤組織及結(jié)直腸腺癌組織中的表達(dá)陽(yáng)性率分別為3.3%(1/30)、30.0%(9/30)及53.3%(32/60)，在3種組織中表達(dá)逐漸增強(qiáng),兩兩比較差異有統(tǒng)計(jì)意義(P lt;0.05)。Fascin-1蛋白在結(jié)直腸腺癌漿膜浸潤(rùn)、淋巴結(jié)轉(zhuǎn)移和TNM Ⅲ+Ⅳ期中的表達(dá)陽(yáng)性率明顯高于無(wú)漿膜浸潤(rùn)、無(wú)淋巴結(jié)轉(zhuǎn)移及TNM Ⅰ+Ⅱ期者(P lt;0.05)，不同性別、年齡及分化程度之間差異無(wú)統(tǒng)計(jì)學(xué)意義(P gt;0.05)。
　結(jié)論 　Fascin-1蛋白表達(dá)與結(jié)直腸腺癌高侵襲能力及淋巴結(jié)轉(zhuǎn)移有關(guān)，可作為潛在預(yù)測(cè)結(jié)直腸癌浸潤(rùn)、轉(zhuǎn)移的敏感指標(biāo)。

引用本文： 李宏偉,王鐵山,黃勇,李艷紅,胡海霞. 結(jié)直腸腺癌中Fascin-1蛋白表達(dá)與其臨床病理特征的關(guān)系. 中國(guó)普外基礎(chǔ)與臨床雜志, 2010, 17(4): 362-364. doi: 復(fù)制

1. 熊正文. 免疫組織化學(xué)技術(shù)中抗原修復(fù)的研究進(jìn)展 [J].中華病理學(xué)雜志, 1997; 26(2): 124-126.
2. 　許良中, 楊文濤. 免疫組織化學(xué)反應(yīng)結(jié)果的判斷標(biāo)準(zhǔn) [J]. 中國(guó)癌癥雜志, 1996; 6(4): 229-231.
3. 　Bryan J, Kane RE. Separation and interaction of themajor components of sea urchin actin gel [J]. J Mol Biol, 1978; 125(2): 207-224.
4. 　Guo W, Giancotti FG. Integrin signalling during tumour progresssion [J]. Nat Rev Mol Cell Biol, 2004; 5(10): 816-826.
5. 　Ono S, Yamakita Y, Yamashim S, et al. Dentification of an actin binding region and a protein kinase C phoep horylation site on human fascin [J]. J Biol Chem, 1997; 272(4): 2527-2533.
6. 　Adams JC, Clelland JD, Collett GD, et al. Cell-matrix adhesions diff-erenttially regulate fascin phosphorylation [J]. Mol Biol Cell, 1999; 10(12): 4177-4190.
7. 　榮舉, 許麗艷, 蔡唯佳, 等. Fasin-1基因在永生化食管上皮細(xì)胞癌變中的表達(dá) [J]. 癌癥, 2004; 23(3): 243-248.
8. 　Hashimoto Y, Ito T, Inoue H, et al. Prognostic significance of fascin overexpression in human esophageal squamouscell carcinoma [J]. Clin Cancer Res, 2005; 11(7): 2597-2605.
9. 　Yoder BJ, Tso E, Skacel M, et al. The expression of fascin, an actin-bunding motility protein, correlates with homone receptor-negative breast cancer and amore aggressi clinical course [J]. Clin Cancer Res, 2005; 11(1): 186-192.
10. 　Xie JJ, Xu LY, Zhang HH, et al. Role of fascin in the proliferation and invasiveness of esophagea carcinoma cells [J]. Biochem Biophys Res Commun, 2005; 337(1): 355-362.
11. 　Pelosi G, Pastorino U, Pasini F, et al. Independent prognostic value of fascin immunoreactivity in stage Ⅰ nonsmall cell lung cancer [J]. Br J Cancer, 2003; 24(88): 537-547.
12. 　Hashimoto Y, Shimada Y, Kawamura J, et al. The prognostic relevance of Fascin expression in human gastric carcinoma [J]. Oncology, 2004; 67(3-4): 262-270.
13. 　楊強(qiáng), 盧朝輝, 羅玉鳳, 等. fascin 蛋白在結(jié)、直腸腺瘤和癌中的表達(dá)及意義 [J]. 診斷病理學(xué)雜志, 2006； 13(5): 359-361.
14. 　高翔, 吳德華. FSCN1蛋白在人上皮性腫瘤組織中的表達(dá)及其臨床意義 [J]. 南方醫(yī)科大學(xué)學(xué)報(bào), 2008; 28(6): 953-955.
15. 　Jawhari AU, Buda A, Jenkins M, et al. Fascin, an actin-bundling protein, modulates colonic epithelial cell invasiveness and differentiation in vitro [J]. Am J Pathol, 2003; 162(1): 69-80.
16. 　Iguchi T, Aishima S, Umeda K, et al. Fascin expression in progression and prognosis of hepatocellular carcinoma [J]. J Surg Oncol, 2009; 100(7): 575-579.
17. 　Kefeli M, Yildiz L, Kaya FC, et al. Fascin expression in uterine smooth muscle tumors [J]. Int J Gynecol Pathol, 2009; 28(4): 328-333.
18. 　Qualtrough D, Singh K, Banu N. The actin-bundling protein fascin is overexpressed in colorectal adenomas and promotes motility in adenoma cells in vitro [J]. Br J Cancer, 2009; 101(7): 1124-1129.
19. 　Tao YS, Edwards RA, Tubb B, et al. beta-Catenin associates with the actin-bunding protein fascin in a noncadherin complex [J]. J Cell Biol, 1996; 134(5): 1271-1281.
20. 　Vignjevic D, Schoumacher M, Gavert N, et al. Fascin, a novel target of beta-catenin-TCF signaling, is expressed at the invasive front of human colon cancer [J]. Cancer Res, 2007; 67(14): 6844-6853.
21. 　Oving IM, Clevers HC. Molecular causes of colon cancer [J]. Eur J Clin Invest, 2002; 32(6): 448-457.
  1. 1. 熊正文. 免疫組織化學(xué)技術(shù)中抗原修復(fù)的研究進(jìn)展 [J].中華病理學(xué)雜志, 1997; 26(2): 124-126.
  2. 2. 　許良中, 楊文濤. 免疫組織化學(xué)反應(yīng)結(jié)果的判斷標(biāo)準(zhǔn) [J]. 中國(guó)癌癥雜志, 1996; 6(4): 229-231.
  3. 3. 　Bryan J, Kane RE. Separation and interaction of themajor components of sea urchin actin gel [J]. J Mol Biol, 1978; 125(2): 207-224.
  4. 4. 　Guo W, Giancotti FG. Integrin signalling during tumour progresssion [J]. Nat Rev Mol Cell Biol, 2004; 5(10): 816-826.
  5. 5. 　Ono S, Yamakita Y, Yamashim S, et al. Dentification of an actin binding region and a protein kinase C phoep horylation site on human fascin [J]. J Biol Chem, 1997; 272(4): 2527-2533.
  6. 6. 　Adams JC, Clelland JD, Collett GD, et al. Cell-matrix adhesions diff-erenttially regulate fascin phosphorylation [J]. Mol Biol Cell, 1999; 10(12): 4177-4190.
  7. 7. 　榮舉, 許麗艷, 蔡唯佳, 等. Fasin-1基因在永生化食管上皮細(xì)胞癌變中的表達(dá) [J]. 癌癥, 2004; 23(3): 243-248.
  8. 8. 　Hashimoto Y, Ito T, Inoue H, et al. Prognostic significance of fascin overexpression in human esophageal squamouscell carcinoma [J]. Clin Cancer Res, 2005; 11(7): 2597-2605.
  9. 9. 　Yoder BJ, Tso E, Skacel M, et al. The expression of fascin, an actin-bunding motility protein, correlates with homone receptor-negative breast cancer and amore aggressi clinical course [J]. Clin Cancer Res, 2005; 11(1): 186-192.
  10. 10. 　Xie JJ, Xu LY, Zhang HH, et al. Role of fascin in the proliferation and invasiveness of esophagea carcinoma cells [J]. Biochem Biophys Res Commun, 2005; 337(1): 355-362.
  11. 11. 　Pelosi G, Pastorino U, Pasini F, et al. Independent prognostic value of fascin immunoreactivity in stage Ⅰ nonsmall cell lung cancer [J]. Br J Cancer, 2003; 24(88): 537-547.
  12. 12. 　Hashimoto Y, Shimada Y, Kawamura J, et al. The prognostic relevance of Fascin expression in human gastric carcinoma [J]. Oncology, 2004; 67(3-4): 262-270.
  13. 13. 　楊強(qiáng), 盧朝輝, 羅玉鳳, 等. fascin 蛋白在結(jié)、直腸腺瘤和癌中的表達(dá)及意義 [J]. 診斷病理學(xué)雜志, 2006； 13(5): 359-361.
  14. 14. 　高翔, 吳德華. FSCN1蛋白在人上皮性腫瘤組織中的表達(dá)及其臨床意義 [J]. 南方醫(yī)科大學(xué)學(xué)報(bào), 2008; 28(6): 953-955.
  15. 15. 　Jawhari AU, Buda A, Jenkins M, et al. Fascin, an actin-bundling protein, modulates colonic epithelial cell invasiveness and differentiation in vitro [J]. Am J Pathol, 2003; 162(1): 69-80.
  16. 16. 　Iguchi T, Aishima S, Umeda K, et al. Fascin expression in progression and prognosis of hepatocellular carcinoma [J]. J Surg Oncol, 2009; 100(7): 575-579.
  17. 17. 　Kefeli M, Yildiz L, Kaya FC, et al. Fascin expression in uterine smooth muscle tumors [J]. Int J Gynecol Pathol, 2009; 28(4): 328-333.
  18. 18. 　Qualtrough D, Singh K, Banu N. The actin-bundling protein fascin is overexpressed in colorectal adenomas and promotes motility in adenoma cells in vitro [J]. Br J Cancer, 2009; 101(7): 1124-1129.
  19. 19. 　Tao YS, Edwards RA, Tubb B, et al. beta-Catenin associates with the actin-bunding protein fascin in a noncadherin complex [J]. J Cell Biol, 1996; 134(5): 1271-1281.
  20. 20. 　Vignjevic D, Schoumacher M, Gavert N, et al. Fascin, a novel target of beta-catenin-TCF signaling, is expressed at the invasive front of human colon cancer [J]. Cancer Res, 2007; 67(14): 6844-6853.
  21. 21. 　Oving IM, Clevers HC. Molecular causes of colon cancer [J]. Eur J Clin Invest, 2002; 32(6): 448-457.