目的 初步研究結(jié)腸切除對(duì)青紫蘭兔結(jié)腸黏膜中5-羥色胺（5-hydroxy tryptamine，5-HT）及嗜鉻粒蛋白A（chromogranin A，CgA）陽性細(xì)胞數(shù)量變化的影響。方法 選取15只青紫蘭兔，切取結(jié)直腸交界上段結(jié)腸7～8 cm長(zhǎng)(對(duì)照組)，繼續(xù)飼養(yǎng)2周后處死動(dòng)物，取吻合口處結(jié)腸段(研究組)。免疫組化法檢測(cè)2組結(jié)腸黏膜中的5-HT陽性細(xì)胞及CgA陽性細(xì)胞，觀察其數(shù)量的變化。結(jié)果 對(duì)照組與研究組結(jié)腸黏膜中的5-HT陽性細(xì)胞數(shù)分別為（10.40±2.22）個(gè)及（26.27±2.35）個(gè)，CgA陽性細(xì)胞數(shù)分別為（20.60±5.34）個(gè)及（51.51±6.13）個(gè)，差異均有統(tǒng)計(jì)學(xué)意義（P＜0.01）。結(jié)論 結(jié)腸切除可引起兔結(jié)腸黏膜中5-HT及CgA陽性細(xì)胞數(shù)量的增多。
引用本文： 錢之遠(yuǎn),涂小煌,陳志耀,馮昌銀. 結(jié)腸切除對(duì)青紫蘭兔結(jié)腸黏膜中5-HT、CgA陽性細(xì)胞的影響. 中國普外基礎(chǔ)與臨床雜志, 2009, 16(6): 452-454. doi: 復(fù)制
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| 2. | 涂小煌， 陳志耀. 補(bǔ)中益氣丸聯(lián)合易蒙停治療大腸癌術(shù)后腹瀉療效觀察 [Ｊ]. 中國中西醫(yī)結(jié)合雜志, 2008; 28(8): 738-741. |
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| 7. | O’Connor DT, Wu H, Gill BM， et al. Hormone storage vesicle proteins. Transcriptional basis of the widespread neuroendocrine expression of chromogranin A, and evidence of its diverse biological actions, intracellular and extracellular [Ｊ]. Ann N Y Acad Sci, 1994; 733: 36-45. |
| 8. | Portela-Gomes GM, Stridsberg M. Chromogranin A in the human gastrointestinal tract: an immunocytochemical study with region-specific antibodies [Ｊ]. J Histochem Cytochem, 2002; 50(11): 1487-1492. |
| 9. | Schürmann G, Bishop AE, Facer P, et al. Secretoneurin: a new peptide in the human enteric nervous system [Ｊ]. Histochem Cell Biol, 1995; 104(1): 11-19. |
| 10. | Ghia JE, Pradaud I, Crenner F, et al. Effect of acetic acid or trypsin application on rat colonic motility in vitro and modulation by two synthetic fragments of chromogranin A [Ｊ]. Regul Pept, 2005; 124(1-3): 27-35. |
| 11. | Albuquerque JF, Ferra MA, Portela-Gomes GM. Adaptive changes of the enterochromaffin and gastrin cells in the rat gastrointestinal tract following subtotal colectomy [Ｊ]. Scand J Gastroenterol, 2006; 41(8): 963-968. |
- 1. Ahlman H, Nilsson. The gut as the largest endocrine organ in the body [Ｊ]. Ann Oncol, 2001; 12 Suppl 2: S63-S68.
- 2. 涂小煌， 陳志耀. 補(bǔ)中益氣丸聯(lián)合易蒙停治療大腸癌術(shù)后腹瀉療效觀察 [Ｊ]. 中國中西醫(yī)結(jié)合雜志, 2008; 28(8): 738-741.
- 3. Watts SW. 5-HT in systemic hypertension: foe, friend or fantasy? [Ｊ]. Clin Sci (Lond), 2005; 108(5): 399-412.
- 4. Spiller R. Serotonin and GI clinical disorders [Ｊ]. Neuropharmacology, 2008; 55(6): 1072-1080.
- 5. Grundy D. 5-HT system in the gut: roles in the regulation of visceral sensitivity and motor functions [Ｊ]. Eur Rev Med Pharmacol Sci, 2008; 12 Suppl 1: 63-67.
- 6. Wiedenmann B, Huttner WB．Synaptophysin and chromogr-anins/secretogranins-widespread constituents of distinct types of neuroendocrine vesicles and new tools in tumor diagnosis [Ｊ]．Virchows Arch B Cell Pathol Incl Mol Pathol, 1989; 58(2): 95-121.
- 7. O’Connor DT, Wu H, Gill BM， et al. Hormone storage vesicle proteins. Transcriptional basis of the widespread neuroendocrine expression of chromogranin A, and evidence of its diverse biological actions, intracellular and extracellular [Ｊ]. Ann N Y Acad Sci, 1994; 733: 36-45.
- 8. Portela-Gomes GM, Stridsberg M. Chromogranin A in the human gastrointestinal tract: an immunocytochemical study with region-specific antibodies [Ｊ]. J Histochem Cytochem, 2002; 50(11): 1487-1492.
- 9. Schürmann G, Bishop AE, Facer P, et al. Secretoneurin: a new peptide in the human enteric nervous system [Ｊ]. Histochem Cell Biol, 1995; 104(1): 11-19.
- 10. Ghia JE, Pradaud I, Crenner F, et al. Effect of acetic acid or trypsin application on rat colonic motility in vitro and modulation by two synthetic fragments of chromogranin A [Ｊ]. Regul Pept, 2005; 124(1-3): 27-35.
- 11. Albuquerque JF, Ferra MA, Portela-Gomes GM. Adaptive changes of the enterochromaffin and gastrin cells in the rat gastrointestinal tract following subtotal colectomy [Ｊ]. Scand J Gastroenterol, 2006; 41(8): 963-968.