• 廣東省中山市人民醫(yī)院普外二科(中山528403);

目的　探討自體外周血干細(xì)胞移植技術(shù)治療下肢動脈缺血性疾病的可行性及存在的問題。方法　
自2004 年3 月至2007 年2 月期間,我院采用自體外周血干細(xì)胞技術(shù)治療16 例患有嚴(yán)重下肢動脈缺血性疾病的患
者,通過采用重組粒細(xì)胞集落刺激因子動員骨髓干細(xì)胞增殖并釋放入血,在動員的第5～6 d ,通過CS23000 PLUS
血細(xì)胞分離機(jī)進(jìn)行外周血干細(xì)胞采集,并將采集到的濃縮干細(xì)胞液做缺血肢體肌肉注射。結(jié)果　經(jīng)治療后3～24
個月的追蹤隨訪,患肢靜息痛緩解,潰瘍愈合,間歇性跛行時間及距離延長。結(jié)論　應(yīng)用自體外周血干細(xì)胞移植技
術(shù)治療下肢動脈缺血性疾病是可行且有效的,是治療缺血性疾病的一種新手段,但該治療方法處在臨床治療的初
級階段,尚需進(jìn)一步研究和探討。

引用本文： 繆健航,師天雄,胡錫祥,孫健鳴,張明光. 自體外周血干細(xì)胞移植治療下肢動脈缺血性疾病的臨床應(yīng)用. 中國普外基礎(chǔ)與臨床雜志, 2008, 15(2): 106-108. doi: 復(fù)制

1. <><><><> [1]錢愛民,李曉強(qiáng),段鵬飛,等.自體外周血干細(xì)胞移植治療老年人慢性下肢缺血臨床研究[J].南通大學(xué)學(xué)報(bào)(醫(yī)學(xué)版),2007; 27(2):109.
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3. 張玉浩,戴坤揚(yáng).內(nèi)皮祖細(xì)胞在下肢缺血性疾病治療中的研究現(xiàn)狀[J].臨床外科雜志,2006; 14(10):668.
4. 黃蓉,夏寧,梁瑜禎.干細(xì)胞移植治療下肢缺血性疾病的研究進(jìn)展[J].中國組織工程研究與臨床康復(fù),2007; 11(7):1305.
5. 王衛(wèi)東,陳正堂.血管內(nèi)皮祖細(xì)胞研究進(jìn)展[J].中國微循環(huán),2002; 6(1):6.
6. 胡詠梅,羅羽慧.骨髓干細(xì)胞移植在心血管疾病中的應(yīng)用[J].心血管病學(xué)進(jìn)展,2003; 24(1):38.
7. 袁紅豐,裴雪濤.內(nèi)皮祖細(xì)胞在心腦血管疾病治療中的應(yīng)用[J].生理科學(xué)進(jìn)展,2003; 34(2):165.
8. 李燕,高平進(jìn),朱鼎良.內(nèi)皮祖細(xì)胞研究進(jìn)展[J].生理科學(xué)進(jìn)展,2002; 33(3):245.
9. Lin Y,Weisdorf DJ,Solovey A,et al.Origins of circulating endothelial cells and endothelial outgrowth from blood[J].J Clin Invest,2000; 105(1):71.
10. Bender JG,Unverzagt KL,Walker DE,et al.Identification and comparison of CD34-positive cells and their subpopulations from normal peripheral blood and bone marrow using multicolor flow cytometry[J].Blood,1991; 77(12):2591.
11. Asahara T,Murohara T,Sullivan A,et al.Isolation of putative progenitor endothelial cells for angiogenesis[J].Science,1997; 275(5302):964.
12. Kalka C,Masuda H,Takahashi T,et al.Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization[J].Proc Natl Acad Sci USA,2000; 97(7):3422.
13. Shintani S,Murohara T,Ikeda H,et al.Mobilization of endothelial progenitor cells in patients with acute myocardial infarction[J].Circulation,2001; 103(23):2776.
14. Isner JM,Asahara T.Angiogenesis and vasculogenesis as therapeutic strategies for postnatal neovascularization[J].J Clin Invest,1999; 103(9):1231.
15. Poole TJ,Finkelstein EB,Cox CM.The role of FGF and VEGF in angioblast induction and migration during vascular development[J].Dev Dyn,2001; 220(1):1.
16. Reyes M,Dudek A,Jahagirdar B,et al.Origin of endothelial progenitors in human postnatal bone marrow[J].J Clin Invest,2002; 109(3):337.
17. Alessandri G,Girelli M,Taccagni G,et al.Human vasculogenesis ex vivo:embryonal aorta as a tool for isolation of endothelial cell progenitors[J].Lab Invest,2001; 81(6):875.
18. Asahara T,Isner JM.Endothelial progenitor cells for vascular regeneration[J].J Hematother Stem Cell Res,2002; 11(2):171.
19. Masuda H,Asahara T.Post-natal endothelial progenitor cells for neovascularization in tissue regeneration[J].Cardiovasc Res,2003; 58(2):390.
  1. 1. <><><><> [1]錢愛民,李曉強(qiáng),段鵬飛,等.自體外周血干細(xì)胞移植治療老年人慢性下肢缺血臨床研究[J].南通大學(xué)學(xué)報(bào)(醫(yī)學(xué)版),2007; 27(2):109.
  2. 2. 趙萍.干細(xì)胞移植治療下肢缺血性疾病的臨床進(jìn)展[J].醫(yī)學(xué)綜述,2007; 13(10):775.
  3. 3. 張玉浩,戴坤揚(yáng).內(nèi)皮祖細(xì)胞在下肢缺血性疾病治療中的研究現(xiàn)狀[J].臨床外科雜志,2006; 14(10):668.
  4. 4. 黃蓉,夏寧,梁瑜禎.干細(xì)胞移植治療下肢缺血性疾病的研究進(jìn)展[J].中國組織工程研究與臨床康復(fù),2007; 11(7):1305.
  5. 5. 王衛(wèi)東,陳正堂.血管內(nèi)皮祖細(xì)胞研究進(jìn)展[J].中國微循環(huán),2002; 6(1):6.
  6. 6. 胡詠梅,羅羽慧.骨髓干細(xì)胞移植在心血管疾病中的應(yīng)用[J].心血管病學(xué)進(jìn)展,2003; 24(1):38.
  7. 7. 袁紅豐,裴雪濤.內(nèi)皮祖細(xì)胞在心腦血管疾病治療中的應(yīng)用[J].生理科學(xué)進(jìn)展,2003; 34(2):165.
  8. 8. 李燕,高平進(jìn),朱鼎良.內(nèi)皮祖細(xì)胞研究進(jìn)展[J].生理科學(xué)進(jìn)展,2002; 33(3):245.
  9. 9. Lin Y,Weisdorf DJ,Solovey A,et al.Origins of circulating endothelial cells and endothelial outgrowth from blood[J].J Clin Invest,2000; 105(1):71.
  10. 10. Bender JG,Unverzagt KL,Walker DE,et al.Identification and comparison of CD34-positive cells and their subpopulations from normal peripheral blood and bone marrow using multicolor flow cytometry[J].Blood,1991; 77(12):2591.
  11. 11. Asahara T,Murohara T,Sullivan A,et al.Isolation of putative progenitor endothelial cells for angiogenesis[J].Science,1997; 275(5302):964.
  12. 12. Kalka C,Masuda H,Takahashi T,et al.Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization[J].Proc Natl Acad Sci USA,2000; 97(7):3422.
  13. 13. Shintani S,Murohara T,Ikeda H,et al.Mobilization of endothelial progenitor cells in patients with acute myocardial infarction[J].Circulation,2001; 103(23):2776.
  14. 14. Isner JM,Asahara T.Angiogenesis and vasculogenesis as therapeutic strategies for postnatal neovascularization[J].J Clin Invest,1999; 103(9):1231.
  15. 15. Poole TJ,Finkelstein EB,Cox CM.The role of FGF and VEGF in angioblast induction and migration during vascular development[J].Dev Dyn,2001; 220(1):1.
  16. 16. Reyes M,Dudek A,Jahagirdar B,et al.Origin of endothelial progenitors in human postnatal bone marrow[J].J Clin Invest,2002; 109(3):337.
  17. 17. Alessandri G,Girelli M,Taccagni G,et al.Human vasculogenesis ex vivo:embryonal aorta as a tool for isolation of endothelial cell progenitors[J].Lab Invest,2001; 81(6):875.
  18. 18. Asahara T,Isner JM.Endothelial progenitor cells for vascular regeneration[J].J Hematother Stem Cell Res,2002; 11(2):171.
  19. 19. Masuda H,Asahara T.Post-natal endothelial progenitor cells for neovascularization in tissue regeneration[J].Cardiovasc Res,2003; 58(2):390.