• 1.四川省德陽(yáng)市人民醫(yī)院普外科（德陽(yáng) 618000）;;
  • 2. 昆明醫(yī)學(xué)院第一附屬醫(yī)院普外科（昆明 650032）;

目的 　探討胰腺炎相關(guān)性腹水（PAAF）與急性化膿性腹膜炎相關(guān)性腹水（ASPAAF）誘導(dǎo)大鼠腸損傷的作用及其可能機(jī)理。
方法 　以PAAF和ASPAAF分別誘導(dǎo)的SD大鼠腸損傷模型為研究對(duì)象，將48只SD大鼠隨機(jī)均分為3組。 對(duì)照組腹腔內(nèi)注射生理鹽水8 ml/只； PAAF組腹腔內(nèi)注射PAAF 8 ml/只， ASPAAF組腹腔內(nèi)注射ASPAAF 8 ml/只。 分別于注射后6 h 和12 h分批處死大鼠，每時(shí)間點(diǎn)8只。 測(cè)定血清內(nèi)毒素和TNF-α水平，同時(shí)測(cè)定小腸組織中Na+-K+-ATP酶活性及TNF-α水平； 于光鏡下觀察腸黏膜損傷情況并作等級(jí)比較。
結(jié)果 　注射后6 h 和12 h， PAAF組和ASPAAF組血清內(nèi)毒素和TNF-α水平, 小腸組織中TNF-α水平以及腸黏膜損傷程度均較對(duì)照組增高(P＜0.05),而Na+-K+-ATP酶活性則顯著降低（P＜0.05）； ASPAAF組血清內(nèi)毒素和TNF-α水平,小腸組織中TNF-α水平以及腸黏膜損傷程度又均較PAAF組增高(P＜0.05),Na+-K+-ATP酶活性則顯著降低（P＜0.05）。
結(jié)論 　PAAF及ASPAAF均能引起大鼠腸損傷，且ASPAAF引起的損傷更為嚴(yán)重。

引用本文： 劉震,程若川,方向,張躍天,趙海劍,李俊,陳玉祥. PAAF與ASPAAF誘導(dǎo)的腸損傷比較的實(shí)驗(yàn)研究. 中國(guó)普外基礎(chǔ)與臨床雜志, 2008, 15(8): 555-559. doi: 復(fù)制

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5. 　Erwin PJ, Lewis IF, Dolan S, et al. Lipopolysaccharide binding protein in acute pancreatitis [J]. Crit Care Med， 2000； 28(1)∶ 104.
6. 　 Makhija R, Kingsnorth AN. Cytockine storm in acute pancreatitis[J]. J Hepatobiliary Pancreat Surg， 2002； 9(4)∶ 401.
7. 　 Davies MG, Hagen PO. Systemic inflammatory response syndrome [J]. Br J Surg, 1997； 84(7)∶ 920.
8. 　 Gomez-Cambronero LG, Sabater L, Pereda J, et al. Role of cytokines and oxidative stress in the pathophysiology of acute pancreatitis: therapeutical implications [J]. Curr Drug Targets Inflamm Allergy, 2002; 1(4)∶ 393.
9. 　 Pastor CM, Frossard JL. Are genetically modified mice useful for the understanding of acute pancreatitis? [J]. FASEB J, 2001; 15(6)∶ 893.
10. 　 Leindler L, Morschl E, Laszlo F, et al. Importance of cytokines, nitric oxide, and apoptosis in the pathological process of necrotizing pancreatitis in rats [J]. Pancreas, 2004; 29(2)∶ 157.
11. 　Ding LA, Li JS. Gut in diseases: physiological elements and their clinical significance[J]. World J Gastroenterol, 2003; 9(11)∶ 2385.
12. 　Liu LR, Xia SH. Role of platelet-activating factor in the pathogenesis of acute pancreatitis[J]. World J Gastroenterol, 2006; 12(4)∶ 539.
13. 　Tracey KJ, Beutler B, Lowry SF, et al. Shock and tissue injury induced by recombinant human cachectin [J]. Science, 1986; 234(4775)∶ 470.
14. 　李紅光, 周總光, 雷松, 等. 人急性胰腺炎早期外周血單核細(xì)胞Toll樣受體4表達(dá)變化 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志, 2006； 13（2）∶191.
15. 　陳哲宇， 齊清會(huì)， 嚴(yán)律南. 細(xì)菌性腹膜炎誘導(dǎo)大鼠多器官功能不全綜合征后結(jié)腸運(yùn)動(dòng)的變化和機(jī)理 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志, 2006； 13（3）∶287.
16. 　劉殿剛, 孫家邦, 李非, 等. 胰腺炎相關(guān)腹水對(duì)大鼠胃腸動(dòng)力的影響 [J]. 北京醫(yī)學(xué), 2005； 27（9）∶546.
17. 　Choudhry MA, Rana SN, Kavanaugh MJ. Impaired intestinal immunity and barrier function: a cause for enhanced bacterial translocation in alcohol intoxication and burn injury[J]. Alcohol, 2004; 33(3)∶ 199.
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19. 　Vliagoftis H, Befus AD, Hollenberg MD, et al. Airway epithelial cells release eosinophil survival-promoting factors (GM-CSF) after stimulation of proteinase-activated receptor 2 [J]. J Allergy Clin Immunol, 2001； 107(4)∶ 679.
20. 　van Pelt LJ, Huisman MV, Weening RS, et al. A single dose of granulocyte-macrophage colony-stimulating factor induces systemic interleukin-8 release and neutrophil activation in healthy volunteers [J]. Blood, 1996； 87(12)∶ 5305.
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23. 　Botha AJ, Moore FA, Moore EE. Early neutrophil sequestration alter injury: a pathogenic mechanism for multiple organ failure [J]. J Trauma， 1995； 39(3)∶ 411.
  1. 1. 　方向， 程若川， 趙海劍， 等． PAAF 誘導(dǎo)腸損傷的實(shí)驗(yàn)研究 [J]. 昆明醫(yī)學(xué)院學(xué)報(bào)， 2005； 26(2)∶ 47.
  2. 2. 　 Lankisch PG, Ihse I. Bile-induced acute experimental pancreatitis [J].Scand J Gastroenterol, 1987； 22(3)∶ 257.
  3. 3. 　 Chiu CJ, McArdle AH, Brown R, et al. Intestinal mucosal lesion in low-flow states. Ⅰ. A morphological, hemodynamic, and metabolic reappraisal [J]. Arch Surg， 1970； 101(4)∶ 478.
  4. 4. 　 Ogle CK, Mao JX, Wu JZ, et al. The 1994 Lindberg Award. The production of tumor necrosis factor, interleukin-1, interleukin-6, and prostaglandin E2 by isolated enterocytes and gut macrophages: effect of lipopolysaccharide and thermal injury[J]. Burn Care Rehabil， 1994； 15(6)∶ 470.
  5. 5. 　Erwin PJ, Lewis IF, Dolan S, et al. Lipopolysaccharide binding protein in acute pancreatitis [J]. Crit Care Med， 2000； 28(1)∶ 104.
  6. 6. 　 Makhija R, Kingsnorth AN. Cytockine storm in acute pancreatitis[J]. J Hepatobiliary Pancreat Surg， 2002； 9(4)∶ 401.
  7. 7. 　 Davies MG, Hagen PO. Systemic inflammatory response syndrome [J]. Br J Surg, 1997； 84(7)∶ 920.
  8. 8. 　 Gomez-Cambronero LG, Sabater L, Pereda J, et al. Role of cytokines and oxidative stress in the pathophysiology of acute pancreatitis: therapeutical implications [J]. Curr Drug Targets Inflamm Allergy, 2002; 1(4)∶ 393.
  9. 9. 　 Pastor CM, Frossard JL. Are genetically modified mice useful for the understanding of acute pancreatitis? [J]. FASEB J, 2001; 15(6)∶ 893.
  10. 10. 　 Leindler L, Morschl E, Laszlo F, et al. Importance of cytokines, nitric oxide, and apoptosis in the pathological process of necrotizing pancreatitis in rats [J]. Pancreas, 2004; 29(2)∶ 157.
  11. 11. 　Ding LA, Li JS. Gut in diseases: physiological elements and their clinical significance[J]. World J Gastroenterol, 2003; 9(11)∶ 2385.
  12. 12. 　Liu LR, Xia SH. Role of platelet-activating factor in the pathogenesis of acute pancreatitis[J]. World J Gastroenterol, 2006; 12(4)∶ 539.
  13. 13. 　Tracey KJ, Beutler B, Lowry SF, et al. Shock and tissue injury induced by recombinant human cachectin [J]. Science, 1986; 234(4775)∶ 470.
  14. 14. 　李紅光, 周總光, 雷松, 等. 人急性胰腺炎早期外周血單核細(xì)胞Toll樣受體4表達(dá)變化 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志, 2006； 13（2）∶191.
  15. 15. 　陳哲宇， 齊清會(huì)， 嚴(yán)律南. 細(xì)菌性腹膜炎誘導(dǎo)大鼠多器官功能不全綜合征后結(jié)腸運(yùn)動(dòng)的變化和機(jī)理 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志, 2006； 13（3）∶287.
  16. 16. 　劉殿剛, 孫家邦, 李非, 等. 胰腺炎相關(guān)腹水對(duì)大鼠胃腸動(dòng)力的影響 [J]. 北京醫(yī)學(xué), 2005； 27（9）∶546.
  17. 17. 　Choudhry MA, Rana SN, Kavanaugh MJ. Impaired intestinal immunity and barrier function: a cause for enhanced bacterial translocation in alcohol intoxication and burn injury[J]. Alcohol, 2004; 33(3)∶ 199.
  18. 18. 　De-Souza DA, Greene LJ. Intestinal permeability and systemic infections in critically ill patients: effect of glutamine [J]. Crit Care Med, 2005; 33(5)∶ 1125.
  19. 19. 　Vliagoftis H, Befus AD, Hollenberg MD, et al. Airway epithelial cells release eosinophil survival-promoting factors (GM-CSF) after stimulation of proteinase-activated receptor 2 [J]. J Allergy Clin Immunol, 2001； 107(4)∶ 679.
  20. 20. 　van Pelt LJ, Huisman MV, Weening RS, et al. A single dose of granulocyte-macrophage colony-stimulating factor induces systemic interleukin-8 release and neutrophil activation in healthy volunteers [J]. Blood, 1996； 87(12)∶ 5305.
  21. 21. 　Frossard JL, Saluja AK, Mach N , et al. In vivo evidence for the role of GM-CSF as a mediated in acute pancreatitis-associated lung injury [J]. Am J Physiol Lung Cell Mol Physiol, 2002； 283(3)∶ L541.
  22. 22. 　ONeill S, ONeill AJ, Conroy E, et al. Altered caspase expression results in delayed neutrophil apoptosis in acute pancreatitis [J]. J Leukoc Biol, 2000； 68(1)∶ 15.
  23. 23. 　Botha AJ, Moore FA, Moore EE. Early neutrophil sequestration alter injury: a pathogenic mechanism for multiple organ failure [J]. J Trauma， 1995； 39(3)∶ 411.