目的 研究S-腺苷蛋氨酸(SAM)預(yù)處理對(duì)大鼠肝臟缺血再灌注損傷線粒體功能的影響。
方法 54只大鼠按隨機(jī)數(shù)字表法隨機(jī)均分為對(duì)照組、缺血再灌注組(I/R組)和SAM組，SAM組大鼠肝臟在缺血前2 h行腹腔注射SAM預(yù)處理。3組動(dòng)物在阻斷肝門30 min后（對(duì)照組僅做分離，不阻斷肝門）復(fù)流，并于再灌注后0、1和6 h抽取下腔靜脈血檢測(cè)ALT及AST，切取肝組織檢測(cè)線粒體SOD、MDA、ATP及EC，并制備病理切片在電鏡下觀察線粒體的超微結(jié)構(gòu)。
結(jié)果 再灌注后0、1及6 h，I/R組ALT、AST和MDA明顯高于對(duì)照組（P＜0.01）， SOD（除0 h外）、ATP及EC明顯低于對(duì)照組（P＜0.01）； SAM組ALT、AST及MDA（除0 h外）明顯低于I/R組（P＜0.01），SOD（除0 h外）、ATP及EC明顯高于I/R組（P＜0.05，P＜0.01）。超微結(jié)構(gòu)觀察，I/R組線粒體較對(duì)照組有明顯的損傷，線粒體數(shù)量減少，腫脹明顯，嵴模糊不清，基質(zhì)密度低； 而SAM組與I/R組相比損傷程度明顯減輕。
結(jié)論 SAM能抑制線粒體脂質(zhì)過氧化反應(yīng)，提高ATP的產(chǎn)生，最終提高線粒體的能量代謝水平，有效地減輕肝臟的缺血再灌注損傷。
引用本文： 郭志剛,何曉順,熊俊,朱曉峰,王東平,錢世鹍,黃潔夫. S-腺苷蛋氨酸對(duì)大鼠缺血再灌注肝臟能量代謝的影響. 中國(guó)普外基礎(chǔ)與臨床雜志, 2007, 14(2): 181-184. doi: 復(fù)制
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