• 武漢協(xié)和醫(yī)院乳腺甲狀腺中心(武漢430022);

目的 　了解在體外條件下不同濃度的雌二醇對不同性質(zhì)的乳腺組織原代細胞增殖的影響。
方法 　用膠原酶消化法獲取乳腺癌患者的各類乳腺組織原代細胞，并用MTT法檢測乳腺癌組織、癌旁組織和正常乳腺組織原代細胞在體外培養(yǎng)下，給予不同濃度雌二醇時細胞的生長增殖情況。
結(jié)果 　雌激素在體外對雌激素受體(ER)表達陽性的乳腺癌組織及癌旁組織原代細胞有明顯的刺激生長作用； 對正常乳腺組織細胞的促生長作用相對較弱，而且與腫瘤組織的ER表達情況無關。
結(jié)論 　當體內(nèi)的雌二醇水平≥103 pmol/L時，乳腺癌的發(fā)病風險和復發(fā)風險會顯著增加。

引用本文： 李治,黃韜,韓明強,田元. 雌二醇對不同性質(zhì)的乳腺原代細胞增殖影響的體外研究. 中國普外基礎與臨床雜志, 2007, 14(3): 255-258. doi: 復制

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  1. 1. Travis RC, Key TJ. Oestrogen exposure and breast cancer risk [J]. Breast Cancer Res, 2003; 5(5)∶239.
  2. 2. Pike MC, Spicer DV, Dahmoush L, et al. Estrogens, progestogens, normal breast cell proliferation, and breast cancer risk [J]. Epidemiol Rev, 1993; 15(1)∶17.
  3. 3. Folkerd EJ, Martin LA, Kendall A, et al. The relationship between factors affecting endogenous oestradiol levels in postmenopausal women and breast cancer [J]. J Steroid Biochem Mol Biol, 2006; 102(1-5)∶250.
  4. 4. Rockhill B, Byrne C, Rosner B, et al. Breast cancer risk prediction with a logincidence model: evaluation of accuracy [J]. J Clin Epidemiol, 2003; 56(9)∶856.
  5. 5. Kabuto M, Akiba S, Stevens RG, et al. A prospective study of estradiol and breast cancer in Japanese women [J]. Cancer Epidemiol Biomarkers Prev, 2000; 9(6)∶575.
  6. 6. Cotterchio M, Kreiger N, Theis B, et al. Hormonal factors and the risk of breast cancer according to estrogenand progesteronereceptor subgroup [J]. Cancer Epidemiol Biomarkers Prev, 2003; 12(10)∶1053.
  7. 7. Jensen J, Kitlen JW, Briand P, et al. Effect of antiestrogens and aromatase inhibitor on basal growth of the human breast cancer cell line MCF7 in serumfree medium [J]. J Steroid Biochem Mol Biol, 2003; 84(4)∶469.
  8. 8. Kuperwasser C, Chavarria T, Wu M, et al. Reconstruction of functionally normal and malignant human breast tissues in mice [J]. Proc Natl Acad Sci USA, 2004; 101(14)∶4966.
  9. 9. Pechoux C, Gudjonsson T, RonnovJessen L, et al. Human mammary luminal epithelial cells contain progenitors to myoepithelial cells [J]. Dev Biol, 1999; 206(1)∶88.
  10. 10. Collaborative Group on Hormonal Factors in Breast Cancer. Breast cancer and hormone replacement therapy: collaborative reanalysis of data from 51 epidemiological studies of 52, 705 women with breast cancer and 108, 411 women without breast cancer [J]. Lancet, 1997; 350(9084)∶1047.
  11. 11. Eerola H, Aittomaki K, AskoSeljavaara S, et al. Hereditary breast cancer and handling of patients at risk [J]. Scand J Surg, 2002; 91(3)∶280.
  12. 12. 郭貴龍， 張筱驊， 姚榛祥. 選擇性COX2抑制劑尼美舒利對大鼠乳腺癌的影響及機理研究 [J]. 中國普外基礎與臨床雜志, 2005; 12（3）∶219.
  13. 13. Clarke RB, Howell A, Potten CS, et al. Dissociation between steroid receptor expression and cell proliferation in the human breast [J]. Cancer Res, 1997; 57(22)∶4987.
  14. 14. 龍啟明， 楊金巧， 陳曉理， 等. 125碘標記17α乙烯雌二醇3醋酸酯在荷人乳腺癌裸鼠體內(nèi)生物學分布 [J]. 中國普外基礎與臨床雜志, 1999; 6(5)∶266.
  15. 15. Khan SA, Rogers MA, Obando JA, et al. Estrogen receptor expression of benign breast epithelium and its association with breast cancer [J]. Cancer Res, 1994; 54(4)∶993.