• 中南大學(xué)湘雅二醫(yī)院肝膽胰疾病研究室（長沙410011）;

目的 　研究胰腺癌和慢性胰腺炎組織中胸苷磷酸化酶（TP）表達水平和淋巴管計數(shù)，探討兩者的臨床病理意義及在胰腺癌中的相互關(guān)系。
方法 　51例胰腺癌和10例慢性胰腺炎手術(shù)切除標(biāo)本常規(guī)制作石蠟包埋切片，TP表達的檢測和淋巴管計數(shù)均采用SP免疫組化法。
結(jié)果 　胰腺癌TP表達陽性率（54.9%）和淋巴管計數(shù)〔（12.5±4.3）　個/HP〕明顯高于慢性胰腺炎TP表達陽性率（20.0%）和淋巴管計數(shù)〔（5.2±2.4）個/HP〕，P＜0.05， P＜0.01；　 高分化胰腺癌和未轉(zhuǎn)移病例TP表達陽性率和淋巴管計數(shù)明顯低于低分化胰腺癌和轉(zhuǎn)移病例（P＜0.05，P＜0.01）； TP表達陽性的胰腺癌淋巴管計數(shù)明顯高于陰性表達者〔(13.8±3.4)個/HP vs （10.9±3.2）個/HP〕，P＜0.01?！?
結(jié)論 　 TP表達和淋巴管計數(shù)均為反映胰腺癌進展、轉(zhuǎn)移等生物學(xué)特性及預(yù)后的重要標(biāo)記物，胰腺癌細胞分泌的TP可能促進淋巴管生成。

引用本文： 楊樂平,楊竹林,梁珊,苗雄鷹,李清龍. 胰腺癌和慢性胰腺炎組織中TP表達和淋巴管計數(shù)的臨床病理意義. 中國普外基礎(chǔ)與臨床雜志, 2007, 14(4): 453-455. doi: 復(fù)制

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2. 　Toi M, Atiqur Rahman M, Bando H, et al. Thymidine phosphorylase (platelet-derived endothelial-cell growth factor) in cancer biology and treatment [Ｊ]. Lancet Oncol, 2005; 6(3)　∶158.
3. 　Terashima M, Fujiwara H, Takagane A, et al. Role of thymidine phosphorylase and dihydropyrimidine dehydrogenase in tumour progression and sensitivity to doxifluridine in gastric cancer patients [Ｊ]. Eur J Cancer, 2002; 38(18)∶ 2375.
4. 　Ciaparrone M, Quirino M, Schinzari G, et al. Predictive role of thymidylate synthase, dihydropyrimidine dehydrogenase and thymidine phosphorylase expression in colorectal cancer patients receiving adjuvant 5-fluorouracil [Ｊ]. Oncology, 2006; 70(5)∶366.
5. 　Erkus M, Meteoglu I, Culhaci N, et al. Does thymidine phosphorylase correlate with angiogenesis in intraductal carcinoma of the breast? [Ｊ]. Saudi Med J, 2006; 27(9)∶1329.
6. 　Kaiserling E. Immunohistochemical identification of lymph vessels with D2-40 in diagnostic pathology [Ｊ]. Pathologe, 2004; 25(5)∶362.
7. 　Van den Eynden GG, Van der Auwera I, Van Laere SJ, et al. Distinguishing blood and lymph vessel invasion in breast cancer: a prospective immunohistochemical study [Ｊ]. Br J Cancer, 2006; 94(11)∶1643.
8. 　Takanami I. Lymphatic microvessel density using D2-40 is associated with nodal metastasis in non-small cell lung cancer [Ｊ]. Oncol Rep, 2006; 15(2)∶437.
9. 　Harrell MI, Iritani BM, Ruddell A. Tumor-induced sentinel lymph node lymphangiogenesis and increased lymph flow precede melanoma metastasis [Ｊ]. Am J Pathol, 2007; 170(2)∶774.
10. 　Sako A, Kitayama J, Ishikawa M, et al. Impact of immunohistochemically identified lymphatic invasion on nodal metastasis in early gastric cancer [Ｊ]. Gastric Cancer, 2006; 9(4)∶295.
11. 　Osaki M, Sakatani T, Okamoto E, et al. Thymidine phosphorylase expression results in a decrease in apoptosis and increase in intratumoral microvessel density in human gastric carcinomas [Ｊ]. Virchows Arch, 2000; 437(1)∶31.
12. 　Shimabukuro T, Matsuyama H, Baba Y, et al. Expression of thymidine phosphorylase in human superficial bladder cancer [Ｊ]. Int J Urol, 2005; 12(1)∶29.
13. 　Chujo M, Miura T, Kawano Y, et al. Thymidine phosphorylase levels and dihydropyrimidine dehydrogenase levels in non-small cell lung cancer tissues [Ｊ]. Oncol Rep, 2006; 16(4)∶777.
14. 　Marks A, Sutherland DR, Bailey D, et al. Characterization and distribution of an oncofetal antigen (M2A antigen) expressed on testicular germ cell tumours [Ｊ]. Br J Cancer, 1999; 80(3-4)∶569　.
15. 　Veikkola T, Jussila L, Makinen T, et al. Signalling via vascular endothelial growth factor receptor-3 is sufficient for lymphangiogenesis in transgenic mice [Ｊ]. EMBO J, 2001; 20(6)∶1223.
16. 　Arigami T, Natsugoe S, Uenosono Y, et al. Lymphatic invasion using D2-40 monoclonal antibody and its relationship to lymph node micrometastasis in pN0 gastric cancer [Ｊ]. Br J Cancer, 2005; 93(6)∶688.
  1. 1. 　Takebayashi Y, Natsugoe S, Baba M, et al. Thymidine phosphorylase in human esophageal squamous cell carcinoma [Ｊ]. Cancer， 1999; 85(2)∶ 282.
  2. 2. 　Toi M, Atiqur Rahman M, Bando H, et al. Thymidine phosphorylase (platelet-derived endothelial-cell growth factor) in cancer biology and treatment [Ｊ]. Lancet Oncol, 2005; 6(3)　∶158.
  3. 3. 　Terashima M, Fujiwara H, Takagane A, et al. Role of thymidine phosphorylase and dihydropyrimidine dehydrogenase in tumour progression and sensitivity to doxifluridine in gastric cancer patients [Ｊ]. Eur J Cancer, 2002; 38(18)∶ 2375.
  4. 4. 　Ciaparrone M, Quirino M, Schinzari G, et al. Predictive role of thymidylate synthase, dihydropyrimidine dehydrogenase and thymidine phosphorylase expression in colorectal cancer patients receiving adjuvant 5-fluorouracil [Ｊ]. Oncology, 2006; 70(5)∶366.
  5. 5. 　Erkus M, Meteoglu I, Culhaci N, et al. Does thymidine phosphorylase correlate with angiogenesis in intraductal carcinoma of the breast? [Ｊ]. Saudi Med J, 2006; 27(9)∶1329.
  6. 6. 　Kaiserling E. Immunohistochemical identification of lymph vessels with D2-40 in diagnostic pathology [Ｊ]. Pathologe, 2004; 25(5)∶362.
  7. 7. 　Van den Eynden GG, Van der Auwera I, Van Laere SJ, et al. Distinguishing blood and lymph vessel invasion in breast cancer: a prospective immunohistochemical study [Ｊ]. Br J Cancer, 2006; 94(11)∶1643.
  8. 8. 　Takanami I. Lymphatic microvessel density using D2-40 is associated with nodal metastasis in non-small cell lung cancer [Ｊ]. Oncol Rep, 2006; 15(2)∶437.
  9. 9. 　Harrell MI, Iritani BM, Ruddell A. Tumor-induced sentinel lymph node lymphangiogenesis and increased lymph flow precede melanoma metastasis [Ｊ]. Am J Pathol, 2007; 170(2)∶774.
  10. 10. 　Sako A, Kitayama J, Ishikawa M, et al. Impact of immunohistochemically identified lymphatic invasion on nodal metastasis in early gastric cancer [Ｊ]. Gastric Cancer, 2006; 9(4)∶295.
  11. 11. 　Osaki M, Sakatani T, Okamoto E, et al. Thymidine phosphorylase expression results in a decrease in apoptosis and increase in intratumoral microvessel density in human gastric carcinomas [Ｊ]. Virchows Arch, 2000; 437(1)∶31.
  12. 12. 　Shimabukuro T, Matsuyama H, Baba Y, et al. Expression of thymidine phosphorylase in human superficial bladder cancer [Ｊ]. Int J Urol, 2005; 12(1)∶29.
  13. 13. 　Chujo M, Miura T, Kawano Y, et al. Thymidine phosphorylase levels and dihydropyrimidine dehydrogenase levels in non-small cell lung cancer tissues [Ｊ]. Oncol Rep, 2006; 16(4)∶777.
  14. 14. 　Marks A, Sutherland DR, Bailey D, et al. Characterization and distribution of an oncofetal antigen (M2A antigen) expressed on testicular germ cell tumours [Ｊ]. Br J Cancer, 1999; 80(3-4)∶569　.
  15. 15. 　Veikkola T, Jussila L, Makinen T, et al. Signalling via vascular endothelial growth factor receptor-3 is sufficient for lymphangiogenesis in transgenic mice [Ｊ]. EMBO J, 2001; 20(6)∶1223.
  16. 16. 　Arigami T, Natsugoe S, Uenosono Y, et al. Lymphatic invasion using D2-40 monoclonal antibody and its relationship to lymph node micrometastasis in pN0 gastric cancer [Ｊ]. Br J Cancer, 2005; 93(6)∶688.