• 西安交通大學醫(yī)學院第一附屬醫(yī)院普通外科（西安710061）;

目的了解上皮型鈣黏附分子（E-cadherin）下調(diào)對腫瘤細胞浸潤能力的影響。
方法選用人胰腺癌細胞株JHP-1，經(jīng)E-cadherin反義寡核苷酸（ASODN）作用，應用免疫細胞化學染色、Western blot法檢測細胞E-cadherin蛋白表達情況，細胞浸潤MTT比色法檢測該細胞對基底膜的浸潤能力。
結(jié)果經(jīng)E-cadherin ASODN作用后， JHP-1細胞膜E-cadherin表達減弱， E-cadherin蛋白含量與對照組相比明顯降低； JHP-1細胞對基底膜浸潤能力明顯高于對照組（P＜0.001）。
結(jié)論E-cadherin與腫瘤細胞的浸潤能力有關(guān)。

引用本文： 車向明,王曙逢,樊林,張如愿,趙偉. E-cadherin反義寡核苷酸對腫瘤細胞浸潤能力影響的研究. 中國普外基礎(chǔ)與臨床雜志, 2006, 13(2): 191-193. doi: 復制

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  3. 3. Xiangming C, Hokita S, Natsugoe S, et al. Cooccurrence of reduced expression of alphacatenin and overexpression of p53 is a predictor of lymph node metastasis in early gastric cancer [Ｊ]. Oncology, 1999; 57(2)∶131.
  4. 4. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding [Ｊ]. Anal Biochem, 1976; 72（1）∶248.
  5. 5. Jansen B, SchlagbauerWadl H, Brown BD, et al. bcl2 antisense therapy chemosensitizes human melanoma in SCID mice [Ｊ]. Nat Med, 1998; 4(2)∶232.
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