• 1.重慶醫(yī)科大學(xué)附屬第一醫(yī)院血管外科、重慶市普外科學(xué)重點(diǎn)實(shí)驗(yàn)室（重慶400016）;;
  • 2.第三軍醫(yī)大學(xué)附屬新橋醫(yī)院普外科（重慶400037）;;
  • 通訊作者： 陳祖林，Email： chenzulin@163.com;

目的觀察內(nèi)源性光動(dòng)力療法(photodynamic therapy, PDT)對(duì)人結(jié)腸癌SW480細(xì)胞環(huán)磷酸腺苷（cAMP）和環(huán)磷酸鳥苷（cGMP）的影響。
方法將SW480細(xì)胞分為4組： 對(duì)照組、 光照組、 δ氨基酮戊酸 (ALA)組、 內(nèi)源性PDT(ALAPDT)組，用放射免疫法檢測(cè)光照后30、60、90及120 min各組細(xì)胞的cAMP和cGMP的濃度。
結(jié)果cAMP濃度在監(jiān)測(cè)時(shí)間內(nèi)，ALAPDT組在30 min時(shí)最高，明顯高于其余各組相應(yīng)時(shí)相及同一組內(nèi)其余時(shí)相（P均＜0.001）； 在60、90及120 min時(shí)與其它各組相應(yīng)時(shí)相比較，差異均無統(tǒng)計(jì)學(xué)意義； 其余各組在監(jiān)測(cè)時(shí)間范圍內(nèi)，組間及組內(nèi)不同時(shí)相間差異均無統(tǒng)計(jì)學(xué)意義。cGMP濃度在各組細(xì)胞的組間及同組內(nèi)不同時(shí)相間差異均無統(tǒng)計(jì)學(xué)意義。
結(jié)論內(nèi)源性PDT在殺傷腫瘤細(xì)胞的同時(shí)，SW480細(xì)胞也能通過細(xì)胞內(nèi)cAMP濃度的瞬時(shí)升高產(chǎn)生保護(hù)作用，抑制細(xì)胞凋亡。

引用本文： 鄭江華,時(shí)德,陳祖林,肖衛(wèi)東,梁平. 內(nèi)源性光動(dòng)力療法對(duì)人結(jié)腸癌SW480細(xì)胞cAMP和cGMP的影響. 中國普外基礎(chǔ)與臨床雜志, 2006, 13(3): 271-274. doi: 復(fù)制

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7. Loweth AC, Williams GT, Scarpello JH, et al. Evidence for the involvement of cGMP and protein kinase G in nitric oxideinduced apoptosis in the pancreatic Bcell line, HITT15 [Ｊ]. FEBS Lett, 1997; 400(3)∶285.
8. Wu CF, Bishopric NH, Pratt RE. Atrial natriuretic peptide induces apoptosis in neonatal rat cardiac myocytes [Ｊ]. J Biol Chem, 1997; 272(23)∶14860.
9. Genaro AM, Hortelano S, Alvarez A, et al. Splenic B lymphocyte programmed cell death is prevented by nitric oxide release through mechanisms involving sustained Bcl2 levels [Ｊ]. J Clin Invest, 1995; 95(4)∶1884.
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11. Peng Q, Berg K, Moan J, et al. 5Aminolevulinic acidbased photodynamic therapy: principles and experimental research [Ｊ]. Photochem Photobiol, 1997; 65(2)∶235.
12. Gahlen J, Stern J, Laubach HH, et al. Improving diagnostic staging laparoscopy using intraperitoneal lavage of deltaaminolevulinic acid (ALA) for laparoscopic fluorescence diagnosis [Ｊ]. Surgery, 1999; 126(3)∶469.
13. 陳祖林， 唐建民主編. 胃腸腫瘤與現(xiàn)代激光 [M]. 北京： 軍事醫(yī)學(xué)科學(xué)出版社，1999∶70～80.
14. Parvathenani LK, Buescher ES, ChaconCruz E, et al. Type Ⅰ cAMPdependent protein kinase delays apoptosis in human neutrophils at a site upstream of caspase3 [Ｊ]. J Biol Chem, 1998; 273(12)∶6736.
15. Wolvetang EJ, Johnson KL, Krauer K, et al. Mitochondrial respiratory chain inhibitors induce apoptosis [Ｊ]. FEBS Lett, 1994; 339(1-2)∶40.
16. Rossi AG, Cousin JM, Dransfield I, et al. Agents that elevate cAMP inhibit human neutrophil apoptosis [Ｊ]. Biochem Biophys Res Commun, 1995; 217(3)∶892.
17. GarciaBermejo L, Perez C, Vilaboa NE, et al. cAMP increasing agents attenuate the generation of apoptosis by etoposide in promonocytic leukemia cells [Ｊ]. J Cell Sci, 1998; 111 (Pt 5)∶637.
18. Penning LC, VanSteveninck J, Dubbelman TM. HPDinduced photodynamic changes in intracellular cyclic AMP levels in human bladder transitional carcinoma cells, clone T24 [Ｊ]. Biochem Biophys Res Commun, 1993; 194(3)∶1084.
19. Inanami O, Yoshito A, Takahashi K, et al. Effects of BAPTAAM and forskolin on apoptosis and cytochrome c release in photosensitized Chinese hamster V79 cells [Ｊ]. Photochem Photobiol, 1999; 70(4)∶650.
  1. 1. Eur J Cancer， 1996; 32A Suppl 5∶S23.
  2. 2. Van Cutsem E. A glimpse of the future. New directions in the treatment of colorectal cancer [Ｊ].
  3. 3. Almeida RD, Manadas BJ, Carvalho AP, et al. Intracellular signaling mechanisms in photodynamic therapy [Ｊ]. Biochim Biophys Acta， 2004; 1704(2)∶59.
  4. 4. Muhl H, Nitsch D, Sandau K, et al. Apoptosis is triggered by the cyclic AMP signalling pathway in renal mesangial cells [Ｊ]. FEBS Lett， 1996; 382(3)∶271.
  5. 5. Lomo J, Blomhoff HK, Beiske K, et al. TGFbeta 1 and cyclic AMP promote apoptosis in resting human B lymphocytes [Ｊ]. J Immunol, 1995; 154(4)∶1634.
  6. 6. Aharoni D, Dantes A, Oren M, et al. cAMPmediated signals as determinants for apoptosis in primary granulosa cells [Ｊ]. Exp Cell Res, 1995; 218(1)∶271.
  7. 7. Loweth AC, Williams GT, Scarpello JH, et al. Evidence for the involvement of cGMP and protein kinase G in nitric oxideinduced apoptosis in the pancreatic Bcell line, HITT15 [Ｊ]. FEBS Lett, 1997; 400(3)∶285.
  8. 8. Wu CF, Bishopric NH, Pratt RE. Atrial natriuretic peptide induces apoptosis in neonatal rat cardiac myocytes [Ｊ]. J Biol Chem, 1997; 272(23)∶14860.
  9. 9. Genaro AM, Hortelano S, Alvarez A, et al. Splenic B lymphocyte programmed cell death is prevented by nitric oxide release through mechanisms involving sustained Bcl2 levels [Ｊ]. J Clin Invest, 1995; 95(4)∶1884.
  10. 10. Johansen LS. Photodynamic therapy. A new method for the treatment of cancer [Ｊ]. Ugeskr Laeger, 1999; 161(26)∶3992.
  11. 11. Peng Q, Berg K, Moan J, et al. 5Aminolevulinic acidbased photodynamic therapy: principles and experimental research [Ｊ]. Photochem Photobiol, 1997; 65(2)∶235.
  12. 12. Gahlen J, Stern J, Laubach HH, et al. Improving diagnostic staging laparoscopy using intraperitoneal lavage of deltaaminolevulinic acid (ALA) for laparoscopic fluorescence diagnosis [Ｊ]. Surgery, 1999; 126(3)∶469.
  13. 13. 陳祖林， 唐建民主編. 胃腸腫瘤與現(xiàn)代激光 [M]. 北京： 軍事醫(yī)學(xué)科學(xué)出版社，1999∶70～80.
  14. 14. Parvathenani LK, Buescher ES, ChaconCruz E, et al. Type Ⅰ cAMPdependent protein kinase delays apoptosis in human neutrophils at a site upstream of caspase3 [Ｊ]. J Biol Chem, 1998; 273(12)∶6736.
  15. 15. Wolvetang EJ, Johnson KL, Krauer K, et al. Mitochondrial respiratory chain inhibitors induce apoptosis [Ｊ]. FEBS Lett, 1994; 339(1-2)∶40.
  16. 16. Rossi AG, Cousin JM, Dransfield I, et al. Agents that elevate cAMP inhibit human neutrophil apoptosis [Ｊ]. Biochem Biophys Res Commun, 1995; 217(3)∶892.
  17. 17. GarciaBermejo L, Perez C, Vilaboa NE, et al. cAMP increasing agents attenuate the generation of apoptosis by etoposide in promonocytic leukemia cells [Ｊ]. J Cell Sci, 1998; 111 (Pt 5)∶637.
  18. 18. Penning LC, VanSteveninck J, Dubbelman TM. HPDinduced photodynamic changes in intracellular cyclic AMP levels in human bladder transitional carcinoma cells, clone T24 [Ｊ]. Biochem Biophys Res Commun, 1993; 194(3)∶1084.
  19. 19. Inanami O, Yoshito A, Takahashi K, et al. Effects of BAPTAAM and forskolin on apoptosis and cytochrome c release in photosensitized Chinese hamster V79 cells [Ｊ]. Photochem Photobiol, 1999; 70(4)∶650.