• 1.四川大學華西醫(yī)院普外二科(成都610041);;
  • 2.大連醫(yī)科大學附屬第一醫(yī)院(大連116011);;
  • 通訊作者: 嚴律南;

【摘要】目的探討細菌性腹膜炎致多器官功能不全綜合征（MODS）大鼠結腸運動的變化和細胞因子白介素6（IL6）、腫瘤壞死因子α（TNFα）及誘生性一氧化氮合成酶（iNOS）對結腸運動的影響。
方法將Wistar大鼠隨機分成正常對照組和MODS組； 分別觀測2組大鼠排便的糞點數(shù)、結腸肌條的振幅、結腸平滑肌細胞長度以及血清一氧化氮（NO）的變化； 利用免疫組化和逆轉錄聚合酶鏈反應（RTPCR）方法檢測結腸平滑肌IL6、TNFα和iNOS表達的變化。
結果MODS組排便的糞點數(shù)和肌條的振幅均明顯低于正常對照組（P＜0.05）； IL6、TNFα和iNOS蛋白表達在正常對照組為陰性表達，在MODS組為陽性表達； 在正常對照組未檢出IL6 mRNA、TNFα mRNA和iNOS mRNA的表達，而在MODS組，IL6 mRNA、TNFα mRNA和iNOS mRNA均呈陽性表達； MODS組平滑肌細胞的長度和血清NO水平均較正常對照組明顯增高（P＜0.01）。
結論MODS后結腸運動功能障礙與iNOS、IL6和TNFα密切相關。

引用本文： 陳哲宇,齊清會,嚴律南. 細菌性腹膜炎誘導大鼠多器官功能不全綜合征后結腸運動的變化和機理. 中國普外基礎與臨床雜志, 2006, 13(3): 287-290. doi: 復制

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  2. 2. 陳哲宇, 齊清會. 多器官功能不全綜合征大鼠結腸運動功能變化和機制的研究 [Ｊ]. 中華實驗外科雜志, 2004; 21(3)∶341.
  3. 3. Hierholzer C, Kalff JC, Chakraborty A, et al. Impaired gut contractility following hemorrhagic shock is accompaied by IL6 and GCSF production and neutrophil infiltration [Ｊ]. Dig Dis Sci, 2001; 46(2)∶230.
  4. 4. Hierholzer C, Kalff JC, Billiar TR, et al. Induced nitric oxide promotes intestinal inflammation following hemorrhagic shock [Ｊ]. Am J Physiol Gastrointest Liver Physiol, 2004; 286(2)∶G225.
  5. 5. De Winter BY. Study of the pathogenesis of paralytic ileus in animal models of experimentally induced postoperative and septic ileus [Ｊ]. Verh K Acad Geneeskd Belg, 2003; 65(5)∶293.
  6. 6. Bani D, Baccari MC, Quattrone S, et al. Relaxin depresses small bowel motility through a nitric oxidemediated mechanism： Studies in mice [Ｊ]. Biol Reprod, 2002; 66(3)∶778.