• 1.南華大學(xué)第二附屬醫(yī)院普外科（湖南衡陽421001）;;
  • 2.中南大學(xué)湘雅二醫(yī)院肝膽外科（長沙410011）;

目的探討多次熱療后殘存的肝癌HepG2細(xì)胞增殖速度變化及其相關(guān)的可能原因。
方法HepG2細(xì)胞43 ℃加熱，每次80 min，2次/d，10個(gè)循環(huán)，分析細(xì)胞倍增時(shí)間，檢測細(xì)胞周期及bcl-2 mRNA及bax mRNA的表達(dá)。
結(jié)果熱處理后的HepG2細(xì)胞增殖加快，細(xì)胞周期中G2期和S期細(xì)胞的比率增高，bcl-2 mRNA的表達(dá)增強(qiáng)，bcl-2/bax比值增大。
結(jié)論熱處理后的HepG2細(xì)胞的增殖加快與其完成DNA復(fù)制而進(jìn)入分裂的細(xì)胞比率高、抗凋亡基因bcl-2 mRNA的強(qiáng)表達(dá)及bcl-2/bax比值的增加有關(guān)。

引用本文： 秦春宏,李永國. 重復(fù)加熱對肝癌HepG2細(xì)胞增殖周期及bcl-2的影響. 中國普外基礎(chǔ)與臨床雜志, 2006, 13(3): 295-297下轉(zhuǎn)304. doi: 復(fù)制

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  2. 2. Beierle EA, Dai W, Iyengar R, et al. Differential expression of bcl2 and bax may enhance neuroblastoma survival [Ｊ]. J Pediatr Surg, 2003; 38(3)∶486.
  3. 3. Pettersson F, Dalgleish AG, Bissonnette RP, et al. Retinoids cause apoptosis in pancreatic cancer cells via activation of RARgamma and altered expression of bcl2/bax [Ｊ]. Br J Cancer, 2002; 87(5)∶555.
  4. 4. Kim JH, Ju EM, Lee DK, et al. Induction of apoptosis by momordin Ⅰ in promyelocytic leukemia (HL60) cells [Ｊ]. Anticancer Res, 2002; 22(3)∶1885.