• 山東大學(xué)齊魯醫(yī)院普外科（濟(jì)南250012）;

目的研究survivin反義寡核苷酸(ASODN)對膽囊癌細(xì)胞凋亡的誘導(dǎo)作用。
方法采用脂質(zhì)體介導(dǎo)survivin ASODN轉(zhuǎn)染人膽囊癌細(xì)胞株GBC-SD，采用流式細(xì)胞儀技術(shù)檢查細(xì)胞凋亡變化，采用電鏡技術(shù)觀察細(xì)胞的形態(tài)變化，采用逆轉(zhuǎn)錄-聚合酶鏈反應(yīng)（RT-PCR）檢測survivin基因表達(dá)的變化。
結(jié)果脂質(zhì)體介導(dǎo)轉(zhuǎn)染survivin ASODN后，膽囊癌細(xì)胞內(nèi)survivin mRNA表達(dá)相對系數(shù)為0.512±0.011,明顯低于未轉(zhuǎn)染者的0.980±0.012 (P＜0.01)，平均下調(diào)了47.83%(P＜0.01)，凋亡細(xì)胞比率為(26.28±3.91)%，明顯高于未轉(zhuǎn)染者的（0.50±0.23）%，P＜0.01，電鏡下可見膽囊癌細(xì)胞呈典型凋亡樣改變。
結(jié)論survivin ASODN轉(zhuǎn)染后能下調(diào)survivin的表達(dá)，可有效誘導(dǎo)GBC-SD細(xì)胞凋亡。

引用本文： 馮立民,姜希宏,烏新林,王建立,壽楠海. survivin反義寡核苷酸(ASODN)誘導(dǎo)膽囊癌細(xì)胞凋亡的研究. 中國普外基礎(chǔ)與臨床雜志, 2006, 13(3): 298-301. doi: 復(fù)制

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  2. 2. Todoroki T, Kawamoto T, Takahashi H, et al. Treatment of gallbladder cancer by radical resection [Ｊ]. Br J Surg, 1999; 86(5)∶622.
  3. 3. Hay BA, Wassarman DA, Rubin GM. Drosophila homologs of baculovirus inhibitor of apoptosis proteins function to block cell death [Ｊ]. Cell, 1995; 83(7)∶1253.
  4. 4. ZangemeisterWittke U, Simon HU. An IAP in action: the multiple roles of survivin in differentiation, immunity and malignancy [Ｊ]. Cell Cycle, 2004; 3(9)∶1121.
  5. 5. Li F, Ambrosini G, Chu EY, et al. Control of apoptosis and mitotic spindle checkpoint by survivin [Ｊ]. Nature, 1998; 396(6711)∶580.
  6. 6. Sarela AI, Macadam RC, Farmery SM, et al. Expression of the antiapoptosis gene, survivin, predicts death from recurrent colorectal carcinoma [Ｊ]. Gut, 2000; 46(5)∶645.
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