• 重慶第三軍醫(yī)大學附屬西南醫(yī)院乳腺中心（重慶400038）;

目的探討人乳腺癌組織中端粒酶逆轉錄酶（hTERT）mRNA和BRCA1蛋白的表達及其二者間的關系。
方法采用RTPCR 和免疫組化方法分別檢測43例乳腺癌組織和40例乳腺癌癌旁組織中的hTERT mRNA和BRCA1蛋白的表達。
結果乳腺癌組織中hTERT mRNA和BRCA1蛋白表達陽性率分別為72.1%（31/43）和34.9%（15/43），hTERT mRNA和BRCA1蛋白在乳腺癌組織及其癌旁組織中表達〔5.0%(2/40),77.5%(31/40)〕的差異有統(tǒng)計學意義(P＜0.05)； 在乳腺癌組織中，hTERT mRNA和BRCA1蛋白的表達呈負相關（r＝-0.995, P＜0.01）。
結論BRCA1蛋白弱表達與乳腺癌hTERT轉錄密切相關，可能參與了乳腺癌發(fā)生機理。

引用本文： 陳莉,姜軍,楊新華,范林軍,陳顯春. 乳腺癌端粒酶逆轉錄酶mRNA和BRCA1蛋白表達及其相互關系. 中國普外基礎與臨床雜志, 2006, 13(4): 434-436. doi: 復制

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  3. 3. Bieche I, Nogues C, Paradis V, et al. Quantitation of hTERT gene expression in sporadic breast tumors with a realtime reverse transcriptionpolymerase chain reaction assay [Ｊ]. Clin Cancer Res， 2000； 6(2)∶452.
  4. 4. Miki Y, Swensen J, ShattuckEidens D, et al. A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1 [Ｊ]. Science， 1994; 266(5182)∶66.
  5. 5. Li H, Lee TH, Avraham H. A novel tricomplex of BRCA1, Nmi, and cMyc inhibits cMycinduced human telomerase reverse transcriptase gene (hTERT) promoter activity in breast cancer [Ｊ]. J Biol Chem， 2002; 277(23)∶20965.
  6. 6. Venkitaraman AR. Cancer susceptibility and the functions of BRCA1 and BRCA2 [Ｊ]. Cell， 2002; 108(2)∶171.
  7. 7. Zhou C, Liu J. Inhibition of human telomerase reverse transcriptase gene expression by BRCA1 in human ovarian cancer cells [Ｊ]. Biochem Biophys Res Commun， 2003; 303(1)∶130.