• 江蘇省肝臟移植中心，南京醫(yī)科大學(xué)第一附屬醫(yī)院肝臟外科（南京210029）;

【摘要】目的觀察誘導(dǎo)血紅素氧合酶-1（HO-1） 生成能否減輕老年供肝移植后缺血再灌注損傷。
方法取老年（16個月齡）SD大鼠5只和成年（3個月齡）大鼠5只，分別測定其肝臟組織中超氧化物歧化酶(SOD)和過氧化氫酶(CAT)的活性及維生素E(Vit E)、維生素C(Vit C)和丙二醛(MDA)含量； 另取老年SD大鼠60只作為供體，隨機(jī)均分為血晶素（Hemin）組和對照組，分別于取肝前24 h腹腔注射Hemin或生理鹽水，然后將其肝臟移植給同種大鼠，同時觀察再灌注后肝臟組織學(xué)變化和細(xì)胞凋亡。
結(jié)果老年大鼠肝臟組織中SOD活性明顯低于成年大鼠，Vit C含量亦少（P＜0.05），而MDA明顯增高（P＜0.05）。用Hemin預(yù)處理的老年SD大鼠供肝在切取前，SOD活性增加，Vit E、Vit C含量增加，MDA含量降低（P＜0.05）； 肝移植后血清ALT明顯降低，肝組織HO-1活性明顯增高，肝臟組織學(xué)檢查示再灌注后凋亡細(xì)胞明顯減少（P＜0.05）。
結(jié)論老年SD大鼠肝臟存在氧化應(yīng)激和脂質(zhì)過氧化狀態(tài)，HO-1可通過改善這種狀態(tài)而減輕其缺血-再灌注損傷。

引用本文： 王科,王學(xué)浩,張峰,李相成,錢曉峰 范燁. 誘導(dǎo)HO-1改善氧化應(yīng)激狀態(tài)減輕老年供肝缺血-再灌注損傷△. 中國普外基礎(chǔ)與臨床雜志, 2005, 12(2): 109-112. doi: 復(fù)制

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  6. 6. Briceno J, Marchal T, Padillo J, et al. Influence of marginal donors on liver preservation injury [Ｊ]. Transplantation, 2002; 74(4)522.
  7. 7. Sass G, Soares MC, Yamashita K, et al. Heme oxygenase1 and its reaction product, carbon monoxide, prevent inflammationrelated apoptotic liver damage in mice [Ｊ]. Hepatology, 2003; 38(4)909.
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  9. 9. Schwartzman RA, Cidlowski JA. Apoptosis: the biochemistry and molecular biology of programmed cell death [Ｊ]. Endocr Rev, 1993; 14(2)133.