【摘要】目的 探討外源性透明質(zhì)酸酶(hyaluronidase, Hyase)對(duì)人乳腺癌細(xì)胞ZR-75-30侵襲潛力及血管形成能力的影響。
方法 通過建立體外侵襲模型和應(yīng)用雙室聯(lián)合培養(yǎng)技術(shù)，觀察外源性Hyase對(duì)ZR-75-30細(xì)胞穿透Matrigel(人工基底膜)的能力以及誘導(dǎo)血管內(nèi)皮細(xì)胞形成血管能力的影響。
結(jié)果 實(shí)驗(yàn)組的穿膜細(xì)胞數(shù)為（70.625±11.64）個(gè)，明顯高于對(duì)照組的（22.125±6.09）個(gè)（P＜0.01）； 實(shí)驗(yàn)組的血管管腔數(shù)目為（34.5±2.4）條，明顯高于對(duì)照組的（8.5±1.5）條（P＜0.01）。
結(jié)論 外源性Hyase有促進(jìn)人乳腺癌細(xì)胞侵襲及誘導(dǎo)血管形成的作用。
引用本文： 王良,任國勝,涂剛,石遠(yuǎn). 外源性透明質(zhì)酸酶對(duì)人乳腺癌細(xì)胞侵襲力及血管形成能力的影響△. 中國普外基礎(chǔ)與臨床雜志, 2005, 12(3): 216-218. doi: 復(fù)制
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- 2. Delpech B, Laquerriere A, Maingonnat C, et al. Hyaluronidase is more elevated in human brain metastases than in primary brain tumours [Ｊ]. Anticancer Res, 2002; 22(4)∶2423.
- 3. Novak U, Stylli SS, Kaye AH, et al. Hyaluronidase2 overexpression accelerates intracerebral but not subcutaneous tumor formation of murine astrocytoma cells [Ｊ]. Cancer Res, 1999; 59(24)∶6246.
- 4. Rahmanian M, Heldin P. Testicular hyaluronidase induces tubular structures of endothelial cells grown in threedimensional collagen gel through a CD44mediated mechanism [Ｊ]. Int J Cancer, 2002; 97(5)∶601.
- 5. Goshen R, Ariel I, Shuster S, et al. Hyaluronan, CD44 and its variant exons in human trophoblast invasion and placental angiogenesis [Ｊ]. Mol Hum Reprod, 1996; 2(9)∶685.