【摘要】目的分析不同部位、分期的結(jié)直腸癌及正常黏膜組織中人乳頭狀瘤病毒(human papilloma virus,HPV)16型 E7的表達狀況。
方法應用PCR及免疫組化方法檢測82例手術(shù)切除的結(jié)直腸癌新鮮標本及距腫瘤近端10 cm以遠的正常黏膜組織中HPV16 E7 DNA及蛋白的表達。
結(jié)果結(jié)直腸癌組織HPV16 E7 DNA表達陽性率為51.22%(42/82),明顯高于正常黏膜組織的4.88%(4/82),P＜0.01; 直腸癌組織中HPV16 E7 DNA表達陽性率為64.10%(25/39),明顯高于升結(jié)腸癌的18.18%(2/11), P＜0.05; HPV16 E7 DNA表達陽性率與Dukes分期有關(guān)(P＜0.01),與癌組織分化程度無關(guān)。免疫組化染色顯示,HPV16 E7蛋白主要為細胞核表達，胞漿也可見少量表達，進一步確認了HPV16的感染，且HPV16 E7蛋白與HPV16 E7基因的表達具有相關(guān)性,免疫組化敏感性較PCR方法為低。
結(jié)論結(jié)直腸癌組織HPV16 E7 DNA及蛋白的表達陽性率明顯高于正常黏膜組織，提示部分結(jié)直腸癌存在HPV16感染。癌灶部位距肛門越近，感染率越高； Dukes分期越晚感染率越高。
引用本文： 張建成,周總光,鄭雪蓮,周斌,李紅光,辜俊,王蓉. 結(jié)直腸癌組織HPV16 E7 DNA及蛋白表達的研究△. 中國普外基礎(chǔ)與臨床雜志, 2005, 12(3): 280-283下轉(zhuǎn)289. doi: 復制
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- 2. McGregor B, Byrne P, Kirgan D, et al. Confirmation of the association of human papilloma virus with human colon cancer [Ｊ]. Am J Surg, 1993; 166(6)∶738.
- 3. Audeau A, Han HW, Johnston MJ, et al. Does human papilloma virus have a role in squamous cell carcinoma of the colon and upper rectum? [Ｊ]. Eur J Surg Oncol, 2002; 28(6)∶657.
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- 5. Bosch FX, Munoz N. The viral etiology of cervical cancer [Ｊ]. Virus Res, 2002; 89(2)∶183.
- 6. Frisch M, Smith E, Grulich A, et al. Cancer in a populationbased cohort of men and women in registered homosexual part（下轉(zhuǎn)第289頁） （上承第283頁）nerships [Ｊ]. Am J Epidemiol, 2003; 157(11)∶966.
- 7. Cheng JY, Sheu LF, Meng CL, et al. Detection of human papilloma virus DNA in colorectal carcinomas by polymerase chain reaction [Ｊ]. Gut, 1995; 37(1)∶87.
- 8. Kirgan D, Manalo P, McGregor B. Immunohistochemical demonstration of human papilloma virus antigen in human colon neoplasms [Ｊ]. J Surg Res, 1990; 48(5)∶397.
- 9. Shah KV, Daniel RW, Simons JW, et al. Investigation of colon cancers for human papilloma virus genomic sequences by polymerase chain reaction [Ｊ]. J Surg Oncol, 1992; 51(1)∶5.
- 10. Cheng JY, Meng CL, Chao CF, et al. Human papilloma virus 16 DNA in NIH3T3 cells transformed by colonic cancer cellular DNA [Ｊ]. Gut, 1993; 34(12)∶1710.
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