• 第四軍醫(yī)大學(xué)西京醫(yī)院肝膽外科（西安710032）;

【摘要】目的探討肝癌、癌旁及正常肝組織中Fas和FasL mRNA表達(dá)水平的變化與肝癌發(fā)生、發(fā)展的關(guān)系及其意義。
方法應(yīng)用半定量RT-PCR的方法檢測(cè)肝癌（40例）、癌旁（40例）及正常肝（25例）組織中Fas及FasL mRNA表達(dá)的相對(duì)含量。
結(jié)果正常肝組織、癌旁及肝癌組織中Fas和FasL mRNA表達(dá)的相對(duì)含量分別為0.792±0.039和0.245±0.043、0.857±0.031和0.429±0.035以及0.473±0.047和0.185±0.041。肝癌組織中Fas mRNA表達(dá)的相對(duì)含量明顯低于正常肝組織和癌旁組織（P＜0.05）； 肝癌組織中FasL mRNA表達(dá)的相對(duì)含量亦明顯低于正常肝組織（P＜0.05）和癌旁組織（P＜0.01），但癌旁組織中FasL mRNA表達(dá)的相對(duì)含量高于正常肝組織中的表達(dá)（P＜0.05）。
結(jié)論肝癌組織不僅通過(guò)低表達(dá)Fas來(lái)逃避機(jī)體的免疫監(jiān)視作用，而且還通過(guò)癌旁組織FasL的高表達(dá)，使與之接觸的淋巴細(xì)胞(表達(dá)Fas)凋亡，最終使肝癌細(xì)胞逃脫機(jī)體的免疫殺傷作用，得以增殖和轉(zhuǎn)移。

引用本文： 李海民,竇科峰,李韌,周景師,帝振宇,張福琴. 肝癌組織中Fas和FasL mRNA表達(dá)水平的變化及其意義. 中國(guó)普外基礎(chǔ)與臨床雜志, 2005, 12(3): 284-286. doi: 復(fù)制

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10. Nagao M, Nakajima Y, Hisanaga M, et al. The alteration of Fas receptor and ligand system in hepatocellular carcinomas: how do hepatoma cells escape from the host immune surveillance in vivo? [Ｊ]. Hepatology, 1999; 30(2)∶413.
11. 梁云,陸斌,李曉冬, 等.肝癌細(xì)胞通過(guò)Fas/FasL途徑觸發(fā)T淋巴細(xì)胞的凋亡 [Ｊ]. 第二軍醫(yī)大學(xué)學(xué)報(bào), 2002; 22(11)∶1021.
  1. 1. Shima Y, Nakao K, Nakashima T, et al. Activation of caspase8 in transforming growth factorbetainduced apoptosis of human hepatoma cells [Ｊ]. Hepatology, 1999; 30(5)∶ 1215.
  2. 2. Suzuki A, Hayashida M, Kawano H, et al. Hepatocyte growth factor promotes cell survival from fasmediated cell death in hepatocellular carcinoma cells via Akt activation and Fasdeathinducing signaling complex suppression [Ｊ]. Hepatology, 2000; 32(4 Pt 1)∶796.
  3. 3. Ito Y, Monden M, Takeda T, et al. The status of Fas and Fas ligand expression can predict recurrence of hepatocellular carcinoma [Ｊ]. Br J Cancer, 2000; 82(6)∶1211.
  4. 4. 張俊峰,石學(xué)濤,衣龍海, 等.Fas/FasL在肝癌免疫逃避中作用的探討 [Ｊ]. 山東醫(yī)藥?內(nèi)科(上半月),2002; 42(16)∶1.
  5. 5. Higaki K, Yano H, Kojiro M. Fas antigen expression and its relationship with apoptosis in human hepatocellular carcinoma and noncancerous tissues [Ｊ]. Am J Pathol, 1996; 149(2)∶429.
  6. 6. You KR, Shin MN, Park RK, et al. Activation of caspase8 during N(4hydroxyphenyl)retinamideinduced apoptosis in Fasdefective hepatoma cells [Ｊ]. Hepatology, 2001; 34(6)∶1119.
  7. 7. 梁云,陸斌,王華菁, 等. p53基因、Fas/FasL在提高肝癌細(xì)胞化療藥物敏感性中的作用 [Ｊ]. 中華實(shí)驗(yàn)外科雜志, 2002; 19(4)∶342.
  8. 8. Srand S, Hofmann WJ, Hug H, et al. Lymphcyte apoptosis induce by CD95(APO1/Fas) Ligandexpressing tumor immune escape [Ｊ]. Science, 1996; 274(4)∶1363.
  9. 9. Roskams T, Libbrecht L, Van Damme B, et al. Fas and Fas ligand: strong coexpression in human hepatocytes surrounding hepatocellular carcinoma; can cancer induce suicide in peritumoural cells? [Ｊ]. J Pathol, 2000; 191(2)∶150.
  10. 10. Nagao M, Nakajima Y, Hisanaga M, et al. The alteration of Fas receptor and ligand system in hepatocellular carcinomas: how do hepatoma cells escape from the host immune surveillance in vivo? [Ｊ]. Hepatology, 1999; 30(2)∶413.
  11. 11. 梁云,陸斌,李曉冬, 等.肝癌細(xì)胞通過(guò)Fas/FasL途徑觸發(fā)T淋巴細(xì)胞的凋亡 [Ｊ]. 第二軍醫(yī)大學(xué)學(xué)報(bào), 2002; 22(11)∶1021.