【摘要】目的 探討保存溫度對(duì)移植胰腺功能的影響，確定大鼠胰腺的最適保存溫度。
方法 采用原位灌注、單純低溫保存及建立同系大鼠異位全胰十二指腸移植模型，按不同保存溫度（0 ℃、4 ℃、8 ℃、12 ℃）進(jìn)行分組，保存6 h后進(jìn)行移植，通過光鏡觀察胰腺組織結(jié)構(gòu)變化，高壓液相色譜法檢測(cè)保存6 h后移植物ATP和總腺苷核苷酸（TAN）含量。移植24 h后，檢測(cè)血糖、血清中脂肪酶及淀粉酶含量，測(cè)定移植胰腺組織中髓過氧化酶(MPO)活性，并進(jìn)行組織學(xué)觀察。
結(jié)果 4 ℃組保存6 h后和移植24 h后的胰腺組織結(jié)構(gòu)損傷明顯輕于其它組。保存6 h后胰腺組織中ATP和TAN水平在4 ℃組、0 ℃組、8 ℃組和12 ℃組依次降低，差異有顯著性意義（P＜0.05）。移植24 h后血糖、血清脂肪酶及胰腺組織中MPO水平在4 ℃組、0 ℃組、8 ℃組和12 ℃組依次增高，差異均有顯著性意義（P＜0.05）。結(jié)論4 ℃對(duì)大鼠胰腺是最適保存溫度，它能夠明顯減輕大鼠胰腺的冷損傷程度。
引用本文： 原春輝,劉永鋒,程穎,趙寧,梁健,何三光. 保存溫度對(duì)移植胰腺功能影響的實(shí)驗(yàn)研究. 中國(guó)普外基礎(chǔ)與臨床雜志, 2005, 12(4): 365-368. doi: 復(fù)制
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- 2. Lee S, Tung KSK, Koopmans H, et al. Pancreaticoduodenal transplantation in the rat [Ｊ]. Transplantation, 1971; 13(6)∶ 421.
- 3. Kwon AH, Inui H, Yoshida K, et al. Pancreatic islet preservation at temperatures just above the freezing point [Ｊ]. Transplant Proc,2003; 35(1)∶ 480.
- 4. Ludwig S, Armann B, Escher E, et al. Pathomorphologic and microcirculatory changes and endothelin1 expression in UWand Celsiorpreserved pancreata in experimental pancreas transplantation [Ｊ]. Transplant Proc,2002; 34(6)∶ 2364.
- 5. Mahmoud MS, Wang P, Chaudry IH. Salutary effects of ATPMgCl2 on altered hepatocyte signal transduction after hemorrhagic shock [Ｊ]. Am J Physiol，1997; 272(6)∶ 1347.
- 6. Lakey JR, Kneteman NM, Rajotte RV, et al. Effect of core pancreas temperature during cadaveric procurement on human islet isolation and functional viability [Ｊ]. Transplantation,2002; 73(7)∶ 1106.