• 第三軍醫(yī)大學(xué)西南醫(yī)院全軍肝膽外科研究所(重慶400038);;
  • 通訊作者： 陳平;

【摘要】目的 了解不同切應(yīng)力下肝竇內(nèi)皮細(xì)胞分泌肝細(xì)胞生長(zhǎng)因子（HGF）、白細(xì)胞介素-6（IL-6）、一氧化氮（NO）及一氧化氮合成酶（NOS）的分泌量，探討切應(yīng)力對(duì)原代培養(yǎng)的大鼠肝竇內(nèi)皮細(xì)胞分泌功能的影響。
方法 構(gòu)建血流動(dòng)力學(xué)模型，實(shí)驗(yàn)分為對(duì)照組（切應(yīng)力為0 dyn/cm2）和實(shí)驗(yàn)組2組,實(shí)驗(yàn)組又按切應(yīng)力不同分為12、24和 48 dyn/cm2 3個(gè)亞組。每組分別在4、8、12及24 h抽取1 ml培養(yǎng)基，利用試劑盒檢測(cè)培養(yǎng)基中HGF、IL-6、NO和NOS濃度。
結(jié)果 在不同切應(yīng)力下,肝竇內(nèi)皮細(xì)胞分泌HGF、IL-6、NO和NOS各不相同，在同一時(shí)相隨著切應(yīng)力的升高,HGF、IL-6、NO和NOS的分泌量也升高，實(shí)驗(yàn)組明顯高于對(duì)照組(P＜0.01），且48 dyn/cm2組明顯高于另兩個(gè)亞組(P＜0.01）； 實(shí)驗(yàn)組作用8、12、24 h時(shí)，HGF、IL-6、No及NOS分泌量明顯高于作用4 h時(shí)。
結(jié)論 體外實(shí)驗(yàn)證實(shí),切應(yīng)力升高時(shí)肝竇內(nèi)皮細(xì)胞分泌HGF、IL-6、NO和NOS的量明顯增加,提示部分肝切除術(shù)后門靜脈壓力急劇升高能促使肝竇內(nèi)皮細(xì)胞活化，分泌大量促肝細(xì)胞再生的細(xì)胞因子和介質(zhì)。

引用本文： 丁紀(jì)明,陳平,張林,朱瑾,熊燕,段世剛,李穎. 切應(yīng)力對(duì)大鼠肝竇內(nèi)皮細(xì)胞分泌功能的影響. 中國(guó)普外基礎(chǔ)與臨床雜志, 2005, 12(5): 455-458下轉(zhuǎn)468. doi: 復(fù)制

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  1. 1. Kahn D, van HoornHickman R, Terblanche J. Liver blood flow after partial hepatectomy in the pig [Ｊ]. J Surg Res, 1984; 37(4)∶290.
  2. 2. LeCouter J, Moritz DR, Li B, et al. Angiogenesisindependent endothelial protection of liver: role of VEGFR1 [Ｊ]. Science, 2003; 299(5608)∶890.
  3. 3. Xu B, Broome U, Uzunel M, et al. Capillarization of hepatic sinusoid by liver endothelial cellreactive autoantibodies in patients with cirrhosis and chronic hepatitis [Ｊ]. Am J Pathol, 2003; 163(4)∶1275.
  4. 4. Mendoza L, Carrascal T, De Luca M, et al. Hydrogen peroxide mediates vascular cell adhesion molecule1 expression from interleukin18activated hepatic sinusoidal endothelium: implications for circulating cancer cell arrest in the murine liver [Ｊ]. Hepatology, 2001; 34(2)∶298.
  5. 5. 朱瑾, 陳平,熊燕, 等. 大鼠肝竇內(nèi)皮細(xì)胞分離、培養(yǎng)及其生物學(xué)特性的初步研究 [Ｊ]. 消化外科， 2004； 3（5）∶350.
  6. 6. Huet PM, Nagaoka MR, Desbiens G, et al. Sinusoidal endothelial cell and hepatocyte death following cold ischemiawarm reperfusion of the rat liver [Ｊ]. Hepatology, 2004; 39(4)∶1110.
  7. 7. Nedredal GI, Elvevold KH, Ytrebo LM, et al. Liver sinusoidal endothelial cells represents an important blood clearance system in pigs [Ｊ]. Comp Hepatol, 2003; 2(1)∶1.
  8. 8. Limmer A, Knolle PA. Liver sinusoidal endothelial cells: a new type of organresident antigenpresenting cell [Ｊ]. Arch Immunol Ther Exp (Warsz), 2001; 49(Suppl 1)∶S7.
  9. 9. Mendoza L, VidalVanaclocha F. Inflammatory response of tumoractivated hepatic sinusoidal endothelium as a target for the screening of metastasis chemopreventive drugs [Ｊ]. Methods Mol Med, 2003; 85∶107.
  10. 10. Thomson RY, Clarke AM. Role of portal blood supply in liver regeneration [Ｊ]. Nature, 1965; 208(8)∶392.
  11. 11. Schoen JM, Wang HH, Minuk GY, et al. Shear stressinduced nitric oxide release triggers the liver regeneration cascade [Ｊ]. Nitric Oxide, 2001; 5(5)∶453.
  12. 12. Kaneko T, Sugimoto H, Inoue S, et al. Doppler ultrasonography for postoperative hepatofugal portal blood flow of the intrahepatic portal vein [Ｊ]. Hepatogastroenterology, 2003; 50(54)∶1825.
  13. 13. Sato Y, Tsukada K, Hatakeyama K. Role of shear stress and immune responses in liver regeneration after a partial hepatectomy [Ｊ]. Surg Today, 1999; 29(1)∶1.
  14. 14. Eguchi S, Yanaga K, Sugiyama N, et al. Relationship between portal venous flow and liver regeneration in patients after living donor rightlobe liver transplantation [Ｊ]. Liver Transpl, 2003; 9(6)∶547.
  15. 15. Hasmall S, James N, Hedley K, et al. Mouse hepatocyte response to peroxisome proliferators: dependency on hepatic nonparenchymal cells and peroxisome proliferator activated receptor alpha (PPARalpha) [Ｊ]. Arch Toxicol, 2001; 75(6)∶357.
  16. 16. Malik R, Selden C, Hodgson H. The role of nonparenchymal cells in liver growth [Ｊ]. Semin Cell Dev Biol, 2002; 13(6)∶425.
  17. 17. Mochida S, Ishikawa K, Toshima K, et al. The mechanisms of hepatic sinusoidal endothelial cell regeneration: a possible communication system associated with vascular endothelial growth factor in liver cells [Ｊ]. J Gastroenterol Hepatol, 1998; 13(Suppl)∶S1.
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  19. 19. Michalopoulos GK, DeFrances MC. Liver regeneration [Ｊ]. Science, 1997; 276(5309)∶60.
  20. 20. Michalopoulos GK. Hepatocyte growth factor in liver growth and differentiation [Ｊ]. J Boil Chem, 1995; 98（11）∶219.
  21. 21. Suzuki A, Iwama A, Miyashita H, et al. Role for growth factors and extracellular matrix in controlling differentiation of prospectively isolated hepatic stem cells [Ｊ]. Development, 2003; 130(11)∶2513.
  22. 22. Daveau M, Scotte M, Francois A, et al. Hepatocyte growth factor, transforming growth factor alpha, and their receptors as combined markers of prognosis in hepatocellular carcinoma [Ｊ]. Mol Carcinog, 2003; 36(3)∶130.
  23. 23. Koniaris LG, McKillop IH, Schwartz SI, et al. Liver regeneration [Ｊ]. J Am Coll Surg, 2003; 197(4)∶634.
  24. 24. Debonera F, Aldeguer X, Shen X, et al. Activation of interleukin6/STAT3 and liver regeneration following transplantation [Ｊ]. J Surg Res, 2001; 96(2)∶289.
  25. 25. Cressman DE, Greenbaum LE, DeAngelis RA, et al. Liver failure and defective hepatocyte regeneration in interleukin6deficient mice [Ｊ]. Science, 1996; 274(5291)∶1379.
  26. 26. Kelm M, Feelisch M, Deussen A, et al. Release of endothelium derived nitric oxide in relation to pressure and flow [Ｊ]. Cardiovasc Res, 1991; 25(10)∶831.
  27. 27. Hortelano S, Dewez B, Genaro AM, et al. Nitric oxide is released in regenerating liver after partial hepatectomy [Ｊ]. Hepatology, 1995; 21(3)∶776.
  28. 28. 李黔寧,應(yīng)大君,戴光明,等. 一種簡(jiǎn)易的平行板流動(dòng)腔系統(tǒng)的應(yīng)用 [Ｊ]. 醫(yī)用生物力學(xué)， 2002； 17（1）∶7.
  29. 29. Frangos JA, Eskin SG, McIntire LV, et al. Flow effects on prostacyclin production by cultured human endothelial cells [Ｊ]. Science, 1985; 227(4693)∶1477.