【摘要】目的 了解不同切應(yīng)力下肝竇內(nèi)皮細(xì)胞分泌肝細(xì)胞生長(zhǎng)因子(HGF)、白細(xì)胞介素-6(IL-6)、一氧化氮(NO)及一氧化氮合成酶(NOS)的分泌量,探討切應(yīng)力對(duì)原代培養(yǎng)的大鼠肝竇內(nèi)皮細(xì)胞分泌功能的影響。
方法 構(gòu)建血流動(dòng)力學(xué)模型,實(shí)驗(yàn)分為對(duì)照組(切應(yīng)力為0 dyn/cm2)和實(shí)驗(yàn)組2組,實(shí)驗(yàn)組又按切應(yīng)力不同分為12、24和 48 dyn/cm2 3個(gè)亞組。每組分別在4、8、12及24 h抽取1 ml培養(yǎng)基,利用試劑盒檢測(cè)培養(yǎng)基中HGF、IL-6、NO和NOS濃度。
結(jié)果 在不同切應(yīng)力下,肝竇內(nèi)皮細(xì)胞分泌HGF、IL-6、NO和NOS各不相同,在同一時(shí)相隨著切應(yīng)力的升高,HGF、IL-6、NO和NOS的分泌量也升高,實(shí)驗(yàn)組明顯高于對(duì)照組(P<0.01),且48 dyn/cm2組明顯高于另兩個(gè)亞組(P<0.01); 實(shí)驗(yàn)組作用8、12、24 h時(shí),HGF、IL-6、No及NOS分泌量明顯高于作用4 h時(shí)。
結(jié)論 體外實(shí)驗(yàn)證實(shí),切應(yīng)力升高時(shí)肝竇內(nèi)皮細(xì)胞分泌HGF、IL-6、NO和NOS的量明顯增加,提示部分肝切除術(shù)后門靜脈壓力急劇升高能促使肝竇內(nèi)皮細(xì)胞活化,分泌大量促肝細(xì)胞再生的細(xì)胞因子和介質(zhì)。
引用本文: 丁紀(jì)明,陳平,張林,朱瑾,熊燕,段世剛,李穎. 切應(yīng)力對(duì)大鼠肝竇內(nèi)皮細(xì)胞分泌功能的影響. 中國(guó)普外基礎(chǔ)與臨床雜志, 2005, 12(5): 455-458下轉(zhuǎn)468. doi: 復(fù)制
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- 1. Kahn D, van HoornHickman R, Terblanche J. Liver blood flow after partial hepatectomy in the pig [J]. J Surg Res, 1984; 37(4)∶290.
- 2. LeCouter J, Moritz DR, Li B, et al. Angiogenesisindependent endothelial protection of liver: role of VEGFR1 [J]. Science, 2003; 299(5608)∶890.
- 3. Xu B, Broome U, Uzunel M, et al. Capillarization of hepatic sinusoid by liver endothelial cellreactive autoantibodies in patients with cirrhosis and chronic hepatitis [J]. Am J Pathol, 2003; 163(4)∶1275.
- 4. Mendoza L, Carrascal T, De Luca M, et al. Hydrogen peroxide mediates vascular cell adhesion molecule1 expression from interleukin18activated hepatic sinusoidal endothelium: implications for circulating cancer cell arrest in the murine liver [J]. Hepatology, 2001; 34(2)∶298.
- 5. 朱瑾, 陳平,熊燕, 等. 大鼠肝竇內(nèi)皮細(xì)胞分離、培養(yǎng)及其生物學(xué)特性的初步研究 [J]. 消化外科, 2004; 3(5)∶350.
- 6. Huet PM, Nagaoka MR, Desbiens G, et al. Sinusoidal endothelial cell and hepatocyte death following cold ischemiawarm reperfusion of the rat liver [J]. Hepatology, 2004; 39(4)∶1110.
- 7. Nedredal GI, Elvevold KH, Ytrebo LM, et al. Liver sinusoidal endothelial cells represents an important blood clearance system in pigs [J]. Comp Hepatol, 2003; 2(1)∶1.
- 8. Limmer A, Knolle PA. Liver sinusoidal endothelial cells: a new type of organresident antigenpresenting cell [J]. Arch Immunol Ther Exp (Warsz), 2001; 49(Suppl 1)∶S7.
- 9. Mendoza L, VidalVanaclocha F. Inflammatory response of tumoractivated hepatic sinusoidal endothelium as a target for the screening of metastasis chemopreventive drugs [J]. Methods Mol Med, 2003; 85∶107.
- 10. Thomson RY, Clarke AM. Role of portal blood supply in liver regeneration [J]. Nature, 1965; 208(8)∶392.
- 11. Schoen JM, Wang HH, Minuk GY, et al. Shear stressinduced nitric oxide release triggers the liver regeneration cascade [J]. Nitric Oxide, 2001; 5(5)∶453.
- 12. Kaneko T, Sugimoto H, Inoue S, et al. Doppler ultrasonography for postoperative hepatofugal portal blood flow of the intrahepatic portal vein [J]. Hepatogastroenterology, 2003; 50(54)∶1825.
- 13. Sato Y, Tsukada K, Hatakeyama K. Role of shear stress and immune responses in liver regeneration after a partial hepatectomy [J]. Surg Today, 1999; 29(1)∶1.
- 14. Eguchi S, Yanaga K, Sugiyama N, et al. Relationship between portal venous flow and liver regeneration in patients after living donor rightlobe liver transplantation [J]. Liver Transpl, 2003; 9(6)∶547.
- 15. Hasmall S, James N, Hedley K, et al. Mouse hepatocyte response to peroxisome proliferators: dependency on hepatic nonparenchymal cells and peroxisome proliferator activated receptor alpha (PPARalpha) [J]. Arch Toxicol, 2001; 75(6)∶357.
- 16. Malik R, Selden C, Hodgson H. The role of nonparenchymal cells in liver growth [J]. Semin Cell Dev Biol, 2002; 13(6)∶425.
- 17. Mochida S, Ishikawa K, Toshima K, et al. The mechanisms of hepatic sinusoidal endothelial cell regeneration: a possible communication system associated with vascular endothelial growth factor in liver cells [J]. J Gastroenterol Hepatol, 1998; 13(Suppl)∶S1.
- 18. Sato T, ElAssal ON, Ono T, et al. Sinusoidal endothelial cell(下轉(zhuǎn)第468頁(yè)) (上承第458頁(yè))proliferation and expression of angiopoietin/Tie family in regenerating rat liver [J]. J Hepatol, 2001; 34(5)∶690.
- 19. Michalopoulos GK, DeFrances MC. Liver regeneration [J]. Science, 1997; 276(5309)∶60.
- 20. Michalopoulos GK. Hepatocyte growth factor in liver growth and differentiation [J]. J Boil Chem, 1995; 98(11)∶219.
- 21. Suzuki A, Iwama A, Miyashita H, et al. Role for growth factors and extracellular matrix in controlling differentiation of prospectively isolated hepatic stem cells [J]. Development, 2003; 130(11)∶2513.
- 22. Daveau M, Scotte M, Francois A, et al. Hepatocyte growth factor, transforming growth factor alpha, and their receptors as combined markers of prognosis in hepatocellular carcinoma [J]. Mol Carcinog, 2003; 36(3)∶130.
- 23. Koniaris LG, McKillop IH, Schwartz SI, et al. Liver regeneration [J]. J Am Coll Surg, 2003; 197(4)∶634.
- 24. Debonera F, Aldeguer X, Shen X, et al. Activation of interleukin6/STAT3 and liver regeneration following transplantation [J]. J Surg Res, 2001; 96(2)∶289.
- 25. Cressman DE, Greenbaum LE, DeAngelis RA, et al. Liver failure and defective hepatocyte regeneration in interleukin6deficient mice [J]. Science, 1996; 274(5291)∶1379.
- 26. Kelm M, Feelisch M, Deussen A, et al. Release of endothelium derived nitric oxide in relation to pressure and flow [J]. Cardiovasc Res, 1991; 25(10)∶831.
- 27. Hortelano S, Dewez B, Genaro AM, et al. Nitric oxide is released in regenerating liver after partial hepatectomy [J]. Hepatology, 1995; 21(3)∶776.
- 28. 李黔寧,應(yīng)大君,戴光明,等. 一種簡(jiǎn)易的平行板流動(dòng)腔系統(tǒng)的應(yīng)用 [J]. 醫(yī)用生物力學(xué), 2002; 17(1)∶7.
- 29. Frangos JA, Eskin SG, McIntire LV, et al. Flow effects on prostacyclin production by cultured human endothelial cells [J]. Science, 1985; 227(4693)∶1477.