• 1.第二軍醫(yī)大學(xué)長征醫(yī)院器官移植中心（上海200003）;;
  • 2.第二軍醫(yī)大學(xué)基礎(chǔ)部生化與分子生物學(xué)系（上海200433）;

摘要】目的 研究人白介素-13（hIL-13）對(duì)牛主動(dòng)脈內(nèi)皮細(xì)胞（BAECs）表達(dá)細(xì)胞間黏附分子-1（ICAM-1）和E-選擇素的影響，為hIL-13應(yīng)用于誘導(dǎo)異種移植免疫耐受、減輕異種移植排斥反應(yīng)提供實(shí)驗(yàn)依據(jù)。
方法 用不同濃度的hIL-13（2、5、10、20及40 ng/ml）孵育 BAECs 2 h后，再與腫瘤壞死因子-α（TNF-α， 4 ng/ml）共孵育6 h或18 h； 應(yīng)用細(xì)胞酶聯(lián)免疫吸附分析方法(Cell-ELISA)檢測BAECs表面的E-選擇素和ICAM-1的表達(dá)； 用MTT方法測定hIL-13對(duì)BAECs活性的影響。
結(jié)果 用hIL-13在5~20 ng/ml濃度內(nèi)預(yù)處理BAECs后，能明顯抑制TNF-α誘導(dǎo)的E-選擇素與ICAM-1的表達(dá)，并呈現(xiàn)一定的劑量依賴性(P＜0.01)。經(jīng)不同濃度hIL-13處理的BAECs活性與未經(jīng)hIL-13處理者相比差異無統(tǒng)計(jì)學(xué)意義。
結(jié)論 hIL-13能明顯抑制BAECs表達(dá)E-選擇素與ICAM-1，誘導(dǎo)異種移植的免疫耐受，且hIL-13對(duì)BAECs無損傷作用。

引用本文： 陳剛,傅志仁,王梁華,焦炳華. 人白介素-13對(duì)牛主動(dòng)脈內(nèi)皮細(xì)胞表達(dá)黏附分子的影響. 中國普外基礎(chǔ)與臨床雜志, 2005, 12(5): 469-472. doi: 復(fù)制

版權(quán)信息： ?四川大學(xué)華西醫(yī)院華西期刊社《中國普外基礎(chǔ)與臨床雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

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11. Montgomery KF, Osborn L, Hession C, et al. Activation of endothelialleukocyte adhesion molecule 1 (ELAM1) gene transcription [Ｊ]. Proc Natl Acad Sci USA, 1991; 88(15)∶6523.
12. Hou J, Baichwal V, Cao Z. Regulatory elements and transcription factors controlling basal and cytokineinduced expression of the gene encoding intercellular adhesion molecule 1 [Ｊ]. Proc Natl Acad Sci USA, 1994； 91(24)∶11641.
13. Martin PL, Davis D, Weiss M, et al. Xenotransplantation of bovine bone marrow stromal cells into pig fetuses: incorporation into skeletal muscle [Ｊ]. Anim Biotechnol, 2001; 12(2)∶183.
14. Yoo JG, Choe SY, Rho GJ. Efficient production of cloned bovine embryos using cdc2 kinase inhibitor [Ｊ]. Reprod Domest Anim, 2003; 38(6)∶444.
15. Lentsch AB, Shanley TP, Sarma V, et al. In vivo suppression of NFkappa B and preservation of I kappa B alpha by interleukin10 and interleukin13 [Ｊ]. J Clin Invest, 1997; 100(10)∶2443.
  1. 1. Macarak EJ, Howard BV, Kefalides NA. Properties of calf endothelial cells in culture [Ｊ]. Lab Invest， 1977; 36(1)∶62.
  2. 2. Minter AJ, Keoshkerian E, Chesterman CN, et al. Fibroblast growth factor and heparin protect endothelial cells from the effects of interleukin 1 [Ｊ]. J Cell Physiol, 1996; 167(2)∶229.
  3. 3. Villarete LH, Remick DG. Nitric oxide regulation of IL8 expression in human endothelial cells [Ｊ]. Biochem Biophys Res Commun, 1995; 211(2)∶671.
  4. 4. Weigel G, Grimm M, Griesmacher A, et al. Adhesion molecule behavior during rejection and infection episodes after heart transplantation [Ｊ]. Clin Chem Lab Med, 2000; 38(5)∶403.
  5. 5. Yamazaki S, Isobe M, Suzuki J, et al. Role of selectindependent adhesion in cardiac allograft rejection [Ｊ]. J Heart Lung Transplant, 1998; 17(10)∶1007.
  6. 6. Satoh S, Thomson AW, Nakamura K, et al. Circulating ICAM1, Eselectin, IL2 receptor, and HLA class I in human small bowel, liver, and small bowelplusliver transplant recipients [Ｊ]. Transplantation, 1995; 60(6)∶558.
  7. 7. Nowaczyk M, Gorski A, Durlik M, et al. Role of adhesion molecules in chronic allograft rejection [Ｊ]. Arch Immunol Ther Exp (Warsz), 1999; 47(6)∶373.
  8. 8. Burke GW, Ciancio G, Cirocco R, et al. Association of interleukin10 with rejectionsparing effect in septic kidney transplant recipients [Ｊ]. Transplantation, 1996; 61(7)∶1114.
  9. 9. Ishido N, Matsuoka J, Matsuno T, et al. Induction of donorspecific hyporesponsiveness and prolongation of cardiac allograft survival by jejunal administration of donor splenocytes [Ｊ]. Transplantation, 1999; 68(9)∶1377.
  10. 10. Chen CC, Rosenbloom CL, Anderson DC, et al. Selective inhibition of Eselectin, vascular cell adhesion molecule1, and intercellular adhesion molecule1 expression by inhibitors of I kappa Balpha phosphorylation [Ｊ]. J Immunol, 1995; 155(7)∶3538.
  11. 11. Montgomery KF, Osborn L, Hession C, et al. Activation of endothelialleukocyte adhesion molecule 1 (ELAM1) gene transcription [Ｊ]. Proc Natl Acad Sci USA, 1991; 88(15)∶6523.
  12. 12. Hou J, Baichwal V, Cao Z. Regulatory elements and transcription factors controlling basal and cytokineinduced expression of the gene encoding intercellular adhesion molecule 1 [Ｊ]. Proc Natl Acad Sci USA, 1994； 91(24)∶11641.
  13. 13. Martin PL, Davis D, Weiss M, et al. Xenotransplantation of bovine bone marrow stromal cells into pig fetuses: incorporation into skeletal muscle [Ｊ]. Anim Biotechnol, 2001; 12(2)∶183.
  14. 14. Yoo JG, Choe SY, Rho GJ. Efficient production of cloned bovine embryos using cdc2 kinase inhibitor [Ｊ]. Reprod Domest Anim, 2003; 38(6)∶444.
  15. 15. Lentsch AB, Shanley TP, Sarma V, et al. In vivo suppression of NFkappa B and preservation of I kappa B alpha by interleukin10 and interleukin13 [Ｊ]. J Clin Invest, 1997; 100(10)∶2443.