• 華西醫(yī)科大學(xué)附屬第一醫(yī)院普外科(成都610041);

目的  探討乳腺癌中CyclinD1基因擴(kuò)增和其蛋白表達(dá)的相關(guān)性及其臨床意義。
方法  應(yīng)用半定量PCR和免疫組化技術(shù)，檢測和分析了乳腺癌及其癌旁組織、乳腺良性組織及正常乳腺標(biāo)本中CyclinD1基因的擴(kuò)增和CyclinD1蛋白的表達(dá)情況。
結(jié)果  62例乳腺癌中，CyclinD1基因擴(kuò)增占22.6%(14/62)，蛋白過度表達(dá)占48.4%(30/62)，二者有一定相關(guān)性，而其它各種乳腺組織的CyclinD1基因擴(kuò)增及蛋白過度表達(dá)與之相比差異有顯著性意義(Ｐ＜0.05)。CyclinD1基因擴(kuò)增及蛋白過度表達(dá)與乳腺癌組織學(xué)分級(jí)呈明顯正相關(guān)，且CyclinD1蛋白過度表達(dá)還與雌孕激素受體狀況呈正相關(guān)。
結(jié)論  乳腺癌中CyclinD1基因擴(kuò)增程度和其蛋白過度表達(dá)雖有一定相關(guān)性，但并不完全一致，說明還存在著其它導(dǎo)致CyclinD1蛋白過度表達(dá)的機(jī)理，CyclinD1蛋白的表達(dá)可能還受雌激素的調(diào)控。對(duì)乳腺癌中CyclinD1基因及其蛋白表達(dá)的檢測有一定臨床意義。

引用本文： 趙俊玲,楊金巧,賴毅. CyclinD1基因擴(kuò)增及其蛋白表達(dá)在乳腺癌中的意義. 中國普外基礎(chǔ)與臨床雜志, 2000, 7(3): 147-149下轉(zhuǎn)163. doi: 復(fù)制

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2. Kyomoto R, Kumazwa H, Toda Y, et al. CyclinD1gene amplification is a more potent prognostic factor than its protein overexpression in human headandneck squamouscell carcinoma 〔Ｊ〕. Int J Cancer, 1997; 74(2)∶576.
3. Bartkova J, Lukas J, Müller H, et al. CyclinD1 protein expression and function in human breast cancer 〔Ｊ〕. Int J Cancer, 1994; 57(1)∶353.
4. Michalides R, Hageman P, Tinteren H, et al. A clinicopathological study on overexpression of CyclinD1 and of p53 in a series of 248 patients with operable breast cancer 〔Ｊ〕. Br J Cancer, 1996; 73(2)∶728.
5. Paul J, Michalides R, Jannink I, et al. CyclinD1 expression in invasive breast cancer 〔Ｊ〕. Am J Pathology, 1997; 150(2)∶705.
6. Zhang SY, Caamano J, Cooper F, et al. Immunohistochemistry of CyclinD1 in human breast cancer 〔Ｊ〕. Am J Clin Pathol, 1994; 102(2)∶695.
7. 沈凱， 唐孝達(dá)， 楊宇如. D類細(xì)胞周期素與癌基因和抑癌基因 〔Ｊ〕. 中華醫(yī)學(xué)遺傳學(xué)雜志， 1996； 13(6)∶361.
8. Surmacz E, Reiss K, Sell C, et al. CyclinD1 messenger RNA is inducible by plateletderived growth factor in culture fibroblasts 〔Ｊ〕. Cancer Res, 1992; 52(19)∶4522.
9. Furlanetto RW, Harwell SE, Firck KK. Insulinlike growth factor1 induces CyclinD1 expression in MG 63 human osteosarcoma cell in vitro 〔Ｊ〕. Mol Endocrinol, 1994; 8(2)∶510.
10. Surmacz E, Marina A, Mary K, et al. Type I insulinlike growth factor receptor function in breast cancer 〔Ｊ〕. Breast Cancer Research and Treatment, 1998; 47(1)∶255.
  1. 1. Kamb A, Gruis NA, Weaver FT, et al. A cell cycle regulator potentially in genesis of many tumor types 〔Ｊ〕. Science, 1994; 264(2)∶436.
  2. 2. Kyomoto R, Kumazwa H, Toda Y, et al. CyclinD1gene amplification is a more potent prognostic factor than its protein overexpression in human headandneck squamouscell carcinoma 〔Ｊ〕. Int J Cancer, 1997; 74(2)∶576.
  3. 3. Bartkova J, Lukas J, Müller H, et al. CyclinD1 protein expression and function in human breast cancer 〔Ｊ〕. Int J Cancer, 1994; 57(1)∶353.
  4. 4. Michalides R, Hageman P, Tinteren H, et al. A clinicopathological study on overexpression of CyclinD1 and of p53 in a series of 248 patients with operable breast cancer 〔Ｊ〕. Br J Cancer, 1996; 73(2)∶728.
  5. 5. Paul J, Michalides R, Jannink I, et al. CyclinD1 expression in invasive breast cancer 〔Ｊ〕. Am J Pathology, 1997; 150(2)∶705.
  6. 6. Zhang SY, Caamano J, Cooper F, et al. Immunohistochemistry of CyclinD1 in human breast cancer 〔Ｊ〕. Am J Clin Pathol, 1994; 102(2)∶695.
  7. 7. 沈凱， 唐孝達(dá)， 楊宇如. D類細(xì)胞周期素與癌基因和抑癌基因 〔Ｊ〕. 中華醫(yī)學(xué)遺傳學(xué)雜志， 1996； 13(6)∶361.
  8. 8. Surmacz E, Reiss K, Sell C, et al. CyclinD1 messenger RNA is inducible by plateletderived growth factor in culture fibroblasts 〔Ｊ〕. Cancer Res, 1992; 52(19)∶4522.
  9. 9. Furlanetto RW, Harwell SE, Firck KK. Insulinlike growth factor1 induces CyclinD1 expression in MG 63 human osteosarcoma cell in vitro 〔Ｊ〕. Mol Endocrinol, 1994; 8(2)∶510.
  10. 10. Surmacz E, Marina A, Mary K, et al. Type I insulinlike growth factor receptor function in breast cancer 〔Ｊ〕. Breast Cancer Research and Treatment, 1998; 47(1)∶255.